世界におけるエキノコックス、テニア条虫の種分化、分子共進化研究

2009 Fiscal Year Annual Research Report

• Project/Area Number: 21256003
• Research Institution: Asahikawa Medical College
• Principal Investigator: 伊藤 亮 Asahikawa Medical College, 医学部, 教授 (70054020)
• Co-Investigator(Kenkyū-buntansha): 中尾 稔 旭川医科大学, 医学部, 准教授 (70155670) ; 中谷 和宏 旭川医科大学, 医学部, 准教授 (70109388) ; 迫 康仁 旭川医科大学, 医学部, 助教 (40312459) ; 柳田 哲矢 旭川医科大学, 医学部, 助教 (40431837) ; 岡本 宗裕 鳥取大学, 農学部, 准教授 (70177096)
• Keywords: エキノコックス条虫 / テニア条虫 / 遺伝子分類 / ミトコンドリア遺伝子 / 核遺伝子 / 地理拡散

Research Abstract

中国(青海省)、モンゴル(ウランバートル)、インドネシア(バリ)、ブラジル(サンパウロ)を訪問し、エキノコックス属、テニア属条虫を入手し、さらにマダガスカルからも有鉤条虫サンプル、ヨーロッパ各国、オーストラリアの共同研究者からも、貴重なサンプルを郵送してもらった。これらのサンプルを用い、複数個のミトコンドリア遺伝子、核遺伝子をマーカーとして用い、分子分類学的再検討を実施した。これまでに判明してきている成績は(1)テニア属条虫も大きく2群に分かれること、(2)人体寄生テニア属条虫3種類のうち、無鉤条虫とアジア条虫の交雑個体がタイ、中国から少なからず発見されたこと、(3)これまでEchinococcus granulosus sensu lato(単包条虫)1種類とみなされていたものは5種類(Echinococcus granulosus sensu strict, E.equinus, E.ortoleppi, E.canadensis, E.felidis)に再分類されること、(4)エキノコックス属条虫はE.multilocularis, E.vogeli, E.oligarthrus, E.shiquicusを合わせ、計10種類に分類できること、(5)北半球に分布しているE.multilocularis(多包条虫)は4群に分かれること、(6)ヒトでの単包虫症をひき起こすE.granulosus s.s.について南米ペルーの個体群と中国新疆ウイグル自治区ならびに青海・四川省に分布している個体群についてhaplotype解析を実施した結果、中国国内、ペルーいずれの地域でも基本タイプは全く同じであること、すなわち、非常に小さな母集団が世界に拡散したことが強く示唆される結果であったこと、(7)中国の青海・四川省に分布している多包条虫のhaplotypeも同様に非常に小さな母集団から派生したと推定される結果が得られたこと、それに引き換え(8)青海・四川省に分布している新種、E.shiquicusのhaplotypeにはbottle neck効果が全く認められず、もともとこのチベット高原に分布していた固有種であること、(9)上記(5)でこれまで3群(北アメリカ、アジア、ヨーロッパ)ならびに、中国の内モンゴルの孤立個体とされていたE.multilocularisがモンゴルからの多包虫症患者サンプル追加により、内モンゴルのhaplotype個体が2例追加され、内モンゴル個体を内モンゴル群として記載した。

Research Products

• [Journal Article] Immunoglobulin G subclass responses to recombinant Em18 in the follow-up of patients with alveolar echinococcosis in different clinical stages (2010)
  • Author(s): Tappe D, Sako Y, Itoh S, Frosch M, Gruner B, Kern P, Ito A
  • Journal Title: Clinical and Vaccine Immunology 17(in press)
  • Peer Reviewed
• [Journal Article] State-of-the-Art : Echinococcus and Taenia : phylogenetic taxonomy and its application to molecular diagnosis (2010)
  • Author(s): Nakao M, Yanagida T, Okamoto M, Knapp J, Nkouawa A, Sako Y, Ito A
  • Journal Title: Infection, Genetics and Evolution 10(in press)
  • Peer Reviewed
• [Journal Article] Expert concensus for the diagnosis and treatment of cystic and alveolar echinococcosis in humans (2010)
  • Author(s): Brunetti E, Kern P, Vuitton DA, Writing Panel for the WHO-IWGE
  • Journal Title: Acta Tropica 114 Pages: 1-16
  • Peer Reviewed
• [Journal Article] Specific IgG responses to recombinant antigen B and Em18 in cystic and alveolar echinococcosis in China (2010)
  • Author(s): Li TY, Ito A, Chen XW, Sako Y, Qiu J, Xiao N, Qiu DC, Nakao M, Craig PS
  • Journal Title: Clinical and Vaccine Immunology 17 Pages: 470-475
  • Peer Reviewed
• [Journal Article] Histopathological, serological and molecular confirmation of alveolar echinococcosis cases in Mongolia (2010)
  • Author(s): Ito A, Agvaandaram G, Myadagsuren N, Bat-Ochir OE, Chuluunbaatar B, Nakaya K, Yanagida T, Sako Y, Nakao M, Ishikawa Y, Davaajav A, Dulmaa N
  • Journal Title: American Journal of Tropical Medicine and Hygiene 82 Pages: 266-269
  • Peer Reviewed
• [Journal Article] Mongolian and Japanese joint conference on "Echinococcosis : diagnosis, treatment and prevention, in Mongolia" June 4, 2009 (2010)
  • Author(s): Gurbadam A, Nyamkhuu D, Nyamkhuu G, Tsendjav A, Sergelen O, Narantuya B, Batsukh Z, Battsetseg G, Oyun-Erdene B, Uranchimeg B, Otgonbaatar D, Temuulen D, Bayarmaa E, Abmed D, Tsogtsaikhan S, Usukhbayar A, Smirmaul K, Gereltuya J, Ito A
  • Journal Title: Parasites and Vectors 3 Pages: 8
  • Peer Reviewed
• [Journal Article] Widespread co-endemicity of human cystic and alveolar echinococcosis on the eastern Tibetan plateau, northwest Sichuan/southeast Qinghai, China (2010)
  • Author(s): Li T, Chen X, Zhen R, Qiu J, Qiu D, Xiao N, Ito A, Wang H, Giraudoux P, Sako Y, Nakao M, Craig PS
  • Journal Title: Acta Tropics 113 Pages: 248-256
  • Peer Reviewed
• [Journal Article] Anomalous proglottids of the fish tapeworm Diphyllobothrium nihonkaiense with abnormal segmentation (2010)
  • Author(s): Yanagida T, Matsuoka H, Nakao M, Ito A.
  • Journal Title: Parasitology International 59(in press)
  • Peer Reviewed
• [Journal Article] Neurocysticercosis : assessing where the infection was acquired from (2010)
  • Author(s): Yanagida T, Yuzawa I, Joshi DD, Sako Y, Nakao M, Nakaya K, Kawano N, Oka H, Fujii K, Ito A
  • Journal Title: Journal of Travel Medicine 17(in press)
  • Peer Reviewed
• [Journal Article] Genetic polymorphisms of Echinococcus tapeworms in China as determined by mitochondrial and nuclear DNA sequences (2010)
  • Author(s): Nakao M, Li T, Han X, Ma X, Xiao N, Qiu J, Wang H, Yanagida T, Mamuti W, Wen H, Moro PL, Giraudoux P, Craig PS, Ito A
  • Journal Title: International Journal for Parasitology 40 Pages: 379-385
  • Peer Reviewed
• [Journal Article] Evidence of hybridization between Taenia saginata and Taenia asiatica (2010)
  • Author(s): Okamoto M, Nakao M, Blair D, Anantaphruti MT, Waikagul J, Ito A
  • Journal Title: Parasitology International 59 Pages: 70-74
  • Peer Reviewed
• [Journal Article] The wandering lesion : cerebral sparganosis (2010)
  • Author(s): Shirakawa K, Yamasaki H, Ito A, Miyajima H
  • Journal Title: Neurology 74 Pages: 180
  • Peer Reviewed
• [Journal Article] Inter- and intra-species characterization of tapeworms of the genus Diphyllobothrium (Cestoda : Diphyllobothriidea) from Switzerland, using nuclear and mitochondrial DNA targets (2010)
  • Author(s): Wicht B, Ruggeri-Bernardi N, Yanagida T, Nakao M, Peduzzi R, Ito A
  • Journal Title: Parasitology International 59 Pages: 35-39
  • Peer Reviewed
• [Journal Article] Echinococcus vogeli infection in a hunter, French Guyana (2009)
  • Author(s): Knapp J, Chirica M, Simonnet C, Grenouillet F, Bart JM, Sako Y, Itoh S, Nakao M, Ito A, Millon L
  • Journal Title: Emerging Infectious Diseases 15 Pages: 2029-2031
  • Peer Reviewed
• [Journal Article] Geographic pattern of genetic variation in the fox tapeworm Echinococcus multilocularis (2009)
  • Author(s): Nakao M, Xiao N, Okamoto M, Yanagida T, Sako Y, Ito A
  • Journal Title: Parasitology International 58 Pages: 384-389
  • Peer Reviewed
• [Journal Article] Serological monitoring of progression of alveolar echinococcosis with multi-organ involvement using recombinant Em18 (2009)
  • Author(s): Ishikawa Y, Sako Y, Itoh S, Ohtake T, Kohgo Y, Matsuno T, Ohsaki Y, Miyokawa N, Nakao M, Nakaya K, Ito A
  • Journal Title: Journal of Clinical Microbiology 47 Pages: 3191-3196
  • Peer Reviewed
• [Journal Article] Molecular Genotyping Reveals Natural Infection of the Ground Squirrel (Spermopilus spp) with Echinococcus granulosus in China (2009)
  • Author(s): Yang YR, Liu R, Bai X, Boufana B, Craig PS, Nakao M, Ito A, Zhang JZ, Giraudoux P, McManus DP
  • Journal Title: PLoS Neglected Tropical Diseases 3 Pages: e518
  • Peer Reviewed
• [Journal Article] Cystic echinococcosis in Turkey : genetic variability and first record of the pig strain (G7) in the country (2009)
  • Author(s): Snabel V, Altintas N, D'Amelio S, Nakao M, Romig T, Yolasigmaz A, Gunes K, Turk M, Busi M, Huttner D, Sevcova D, Ito A, Altintas N, Dubinsky P
  • Journal Title: Parasitology Research 105 Pages: 145-154
  • Peer Reviewed
• [Journal Article] Molecular identification of Echinococcus isolates from Peru (2009)
  • Author(s): Moro PL, Nakao M, Ito A, Schantz PM, Cavero C, Cabrera L
  • Journal Title: Parasitology International 58 Pages: 184-186
  • Peer Reviewed
• [Journal Article] Close correlation of clinical regression and specific serology in the follow-up of patients with alveolar echinococcosis in different clinical stages (2009)
  • Author(s): Tappe D, Sako Y, Itoh S, Frosch M, Gruner B, Reuter S, Nakao M, Ito A, Kern P
  • Journal Title: American Journal of Tropical Medicine and Hygiene 80 Pages: 792-797
  • Peer Reviewed
• [Journal Article] Paragonimiasis in Cameroon : molecular identification, serodiagnosis and clinical manifestations (2009)
  • Author(s): Nkouawa A, Okamoto M, Mabou AK, Edinga E, Yamasaki H, Sako Y, Nakao M, Nakaya K, Blair D, Agatsuma T, Enyong P, Shibahara T, Moyou-Somo R, Ito A
  • Journal Title: Transactions of the Royal Society of Tropical Medicine and Hygiene 103 Pages: 255-261
  • Peer Reviewed
• [Journal Article] Loop-mediated isothermal amplification method for differentiation and rapid detection of Taenia species (2009)
  • Author(s): Nkouawa A, Sako Y, Nakao M, Nakaya K, Ito A
  • Journal Title: Journal of Clinical Microbiology 47 Pages: 168-174
  • Peer Reviewed
• [Journal Article] Development of a rapid immunochromatographic test for the diagnosis of alveolar echinococcosis (2009)
  • Author(s): Sako Y, Fukuda K, Kobayashi Y, Ito A
  • Journal Title: Journal of Clinical Microbiology 47 Pages: 252-254
  • Peer Reviewed
• [Presentation] The Recent advances in serodiagnosis of echinococcosis (2009)
  • Author(s): Ito A
  • Organizer: 23rd International Congress of Hydatidology
  • Place of Presentation: Colonia, Uruguay
  • Year and Date: 2009-12-10
• [Presentation] Phylogeny and phylogeography of Echinococcus (2009)
  • Author(s): Ito A
  • Organizer: 23rd International Congress of Hydatidology
  • Place of Presentation: Colonia, Uruguay
  • Year and Date: 2009-12-10
• [Presentation] Phylogeny and phylogeography of human taeniid tapeworms (2009)
  • Author(s): Nakao M
  • Organizer: 6^<th> Seminar on Food- and Water-borne Parasitic Zoonoses
  • Place of Presentation: Bangkok, Thailand
  • Year and Date: 2009-12-04
• [Presentation] Cross-fertilization and hybridization in Taenia species (2009)
  • Author(s): Okamoto M
  • Organizer: 6^<th> Seminar on Food- and Water-borne Parasitic Zoonoses
  • Place of Presentation: Bangkok, Thailand
  • Year and Date: 2009-12-04
• [Presentation] Molecular identification and subtyping of food- and water-borne cestodes (2009)
  • Author(s): Yanagida T
  • Organizer: 6^<th> Seminar on Food- and Water-borne Parasitic Zoonoses
  • Place of Presentation: Bangkok, Thailand
  • Year and Date: 2009-12-04
• [Presentation] Evaluation of a loop-mediated isothermal amplification method using fecal specimens for differential detection of taeniid eggs (2009)
  • Author(s): Nkouawa A
  • Organizer: Joint International Tropical Medicine Meeting 2009
  • Place of Presentation: Bangkok, Thailand
  • Year and Date: 2009-12-04
• [Presentation] Advances in Diagnosis of Neurocysticercosis (2009)
  • Author(s): Ito A
  • Organizer: 1^<st> Indonesian Congress of Neurological Diseases
  • Place of Presentation: Denpasar, Indonesia
  • Year and Date: 2009-11-14
• [Presentation] Present situation of cysticercosis/neurocysticercosis in Indonesia (2009)
  • Author(s): Wandra T
  • Organizer: 1^<st> Indonesian Congress of Neurological Diseases
  • Place of Presentation: Denpasar, Indonesia
  • Year and Date: 2009-11-14
• [Presentation] Echinococcosis : detection of patients and identification of parasites (2009)
  • Author(s): Ito A
  • Organizer: The 2^<nd> Baltic Forum on Zoonoses
  • Place of Presentation: St Peterburg, Russia
  • Year and Date: 2009-10-02
• [Presentation] Immuno- and Molecular-diagnosis of Echinococcosis (2009)
  • Author(s): Ito A
  • Organizer: 10^<th> International Congress of the Asian Society of Clinical Pathology and Laboratory Medicine
  • Place of Presentation: Ulaanbaatar, Mongolia
  • Year and Date: 2009-09-10
• [Book] Handbook of Nucleic Acid Purification (ed. by Liu D) (2009)
  • Author(s): Okamoto M, Ito A
  • Total Pages: 584
  • Publisher: CRC Press, Taylor & Francis Group
• [Book] Foodborn Pathogens (ed. by Liu D) (2009)
  • Author(s): Ito A, Nakao M, Sako Y, Nakaya K, Okamoto M, Yanagida T
  • Total Pages: 879
  • Publisher: CRC Press, Taylor & Francis Group