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2011 Fiscal Year Final Research Report

Glutamate receptor dynamism in the brain functions and diseases

Research Project

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Project/Area Number 21300118
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Neuroscience in general
Research InstitutionNiigata University

Principal Investigator

SAKIMURA Kenji  新潟大学, 脳研究所, 教授 (40162325)

Co-Investigator(Kenkyū-buntansha) NATSUME Rie  新潟大学, 脳研究所, 技術職員 (60467082)
Co-Investigator(Renkei-kenkyūsha) ABE Manabu  新潟大学, 脳研究所, 准教授 (10334674)
YAMAZAKI Maya  新潟大学, 脳研究所, 助教 (70401768)
WATANABE Masahiko  北海道大学, 大学院・医学研究科, 教授 (70210945)
Research Collaborator KANO Masanobu  東京大学, 大学院・医学系研究科, 教授 (40185963)
Project Period (FY) 2009 – 2011
Keywords分子 / 細胞神経科学
Research Abstract

The objective of this study is to verify a working hypothesis that the expression and stability of glutamate receptors, including(further) transition to synapses and removal, are responsible for(are involved in) different modes of neuronal regulation depending on each kind of brain cells and regions, which transforms simple inputs into various types of outputs, thus underlying complex neuronal expressions. For this purpose, we established floxed mice of 4 AMPA-type-and 4 NMDA-type-glutamate receptors, as well as several kinds of synaptic functional molecules floxed mice. We also generated Cre-driver mice such as GAD67-Cre mouse. By inter-crossing these mice, we have clarified that a GluN2B subunit is crucial for synaptic functional expression of NMDA-type receptors in a CA3 area of hippocampus, and that TARPγ-2 andγ-7 are essential for the expression of AMPA type receptors in cerebellum. We have also shown that at developing synapses GluN2A and GluN2B suppress AMPA receptors in their own mode, by using a single-cell NMDA receptor subunit deleted mouse.

  • Research Products

    (29 results)

All 2012 2011 2010 2009 Other

All Journal Article (16 results) (of which Peer Reviewed: 16 results) Presentation (11 results) Remarks (1 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Ablation of NG2 Proteoglycan Leads to Deficits in Brown Fat Function and to Adult Onset Obesity2012

    • Author(s)
      Chang Y, She ZG, Sakimura K, Stallcup WB.
    • Journal Title

      PLoS One

      Volume: 7(1) Pages: e30637

    • Peer Reviewed
  • [Journal Article] Cav2. 1 in Cerebellar Purkinje Cells Regulates Competitive Excitatory Synaptic Wiring2012

    • Author(s)
      Miyazaki T, (他3名) Yamazaki M, Abe M, Kano M, Sakimura K, Watanabe M.
    • Journal Title

      J Neurosci

      Volume: 32(4) Pages: 1311-1328

    • Peer Reviewed
  • [Journal Article] Distinct Modes of AMPA Receptor Suppression at Developing Synapses by GluN2A and GluN2B : Single-Cell NMDA Receptor Subunit Deletion In Vivo2011

    • Author(s)
      Gray JA, (他3名) Sakimura K, Nicoll RA.
    • Journal Title

      Neuron

      Volume: 71(6) Pages: 1085-101

    • Peer Reviewed
  • [Journal Article] Postsynaptic P/Q-type Ca2+channel in Purkinje cell mediates synaptic competition and elimination in developing cerebellum2011

    • Author(s)
      Hashimoto K, (他3名) Yamazaki M, Shin Hashimoto K, (他3名) Yamazaki M, Shin HS, Watanabe M, Sakimura K, Kano M.
    • Journal Title

      Proc Natl Acad Sci USA

      Volume: 108(24) Pages: 9987-92

    • Peer Reviewed
  • [Journal Article] Molecular and morphological configuration for 2-arachidonoylglycerol-mediatedretrograde signaling at mossy cell-granule cell synapses in the dentate gyrus.2011

    • Author(s)
      Uchigashima M, Yamazaki M, (他2名) Sakimura K, Kano M, Watanabe M.
    • Journal Title

      J Neurosci

      Volume: 31(21) Pages: 7700-14

    • Peer Reviewed
  • [Journal Article] Tangential migration and proliferation of intermediate progenitors of GABAergic neurons in the mouse telencephalon2011

    • Author(s)
      Wu S, (他9名) Sakimura K, Kaneko T, Tamamaki N.
    • Journal Title

      Development

      Volume: 138(12) Pages: 2499-509

    • Peer Reviewed
  • [Journal Article] Glutamate receptorδ2 is essential for input pathway-dependent regulation of synaptic AMPAR contents in cerebellar purkinje cells2011

    • Author(s)
      Yamasaki M, , Abe M, Natsume R, (他4名) Mishina M, Sakimura K, Watanabe M.
    • Journal Title

      J Neurosci

      Volume: 31(9) Pages: 3362-3374

    • Peer Reviewed
  • [Journal Article] NG2 cells are uniformly distributed and NG2 is not required for barrel formation in the somatosensory cortex2011

    • Author(s)
      Hill RA, Natsume R, Sakimura K, Nishiyama A.
    • Journal Title

      Mol Cell Neurosci

      Volume: 46(4) Pages: 689-98

    • Peer Reviewed
  • [Journal Article] Unique inhibitory synapse with particularly rich endocannabinoid signaling machinery on pyramidal neurons in basal amygdaloid nucleus.2011

    • Author(s)
      Yoshida T, (他3名) Yamazaki M, Sakimura K, Kano M, Yoshioka M, Watanabe M.
    • Journal Title

      Proc Natl Acad Sci USA

      Volume: 108(7) Pages: 3059-64

    • Peer Reviewed
  • [Journal Article] Ablation of Glutamate Receptor GluR{ delta} 2 in Adult Purkinje Cells Causes Multiple Innervation of Climbing Fibers by Inducing Aberrant Invasion to Parallel Fiber Innervation Territory2010

    • Author(s)
      Miyazaki T, (他2名) Sakimura K, Mishina M, Watanabe M.
    • Journal Title

      J Neurosci

      Volume: 30(45) Pages: 15196-15209

    • Peer Reviewed
  • [Journal Article] Trans-Synaptic Interaction of GluRdelta2 and Neurexin through Cbln1 Mediates Synapse Formation in the Cerebellum2010

    • Author(s)
      Uemura T, (他6名) Sakimura K, Mishina M.
    • Journal Title

      Cell

      Volume: 141(6) Pages: 1068-79

    • Peer Reviewed
  • [Journal Article] TARPsγ-2 and-γ7 are essential for AMPA receptor expression in the cerebellum2010

    • Author(s)
      Yamazaki M, (他5名) Abe M, Natsume R, Takahashi M, Kano M, Sakimura K, Watanabe M.
    • Journal Title

      Eur J Neurosci

      Volume: 31(12) Pages: 2204-20

    • Peer Reviewed
  • [Journal Article] The endocannabinoid 2-arachidonoylglycerol produced by diacylglycerol lipase alpha mediates retrograde suppression of synaptic transmission2010

    • Author(s)
      Tanimura A, Yamazaki M, (他3名) Abe M, Kita Y, Hashimoto K, Shimizu T, Watanabe M, Sakimura K, Kano M.
    • Journal Title

      Neuron

      Volume: 65(3) Pages: 320-327

    • Peer Reviewed
  • [Journal Article] Reduced immobilizing properties of isoflurane and nitrous oxide in mutant mice lacking theN-methyl-D-aspartate receptor GluRepsilon1 subunit are caused by the secondary effects of gene knockout2009

    • Author(s)
      Petrenko AB, (他2名) Sakimura K, Baba H.
    • Journal Title

      Anesth Analg

      Volume: 110(2) Pages: 461-5

    • Peer Reviewed
  • [Journal Article] Subtypes of GABAergic neurons project axons in th neocortex2009

    • Author(s)
      Higo S, Akashi K, Sakimura K, and Tamamaki N
    • Journal Title

      Frontiers in Neuroanatomy

      Volume: 3(25)

    • Peer Reviewed
  • [Journal Article] NMDA receptor GluN2B(GluR epsilon 2/NR2B) subunit is crucial for channel function, postsynaptic macromolecular organization, and actin cytoskeleton at hippocampal CA3 synapses2009

    • Author(s)
      Akashi K, (他4名) Abe M, Natsume R, Watanabe M, Sakimura K.
    • Journal Title

      J Neurosci

      Volume: 29(35) Pages: 10869-82

    • Peer Reviewed
  • [Presentation] Systematic generation of gene manipulated mice using C57BL/6 ES cell RENKA2012

    • Author(s)
      Kenji Sakimura
    • Organizer
      第42回生理研国際シンポジウム
    • Place of Presentation
      愛知県岡崎市生理学研究所
    • Year and Date
      2012-03-06
  • [Presentation] カイニン酸受容体の定量的解析Quantitative analysis of kainate receptor subunits in the mouse brain2011

    • Author(s)
      渡辺和泉
    • Organizer
      第34回日本神経科学大会
    • Place of Presentation
      神奈川県横浜市
    • Year and Date
      2011-09-17
  • [Presentation] Quantitative analysis of Glutamate receptor subunits in the mouse brain. ISN(国際神経化学会)2011

    • Author(s)
      Sakimura K.
    • Organizer
      23rd. Biennial Meeting
    • Place of Presentation
      ギリシャ・アテネ
    • Year and Date
      2011-08-30
  • [Presentation] Transmembrane AMPA receptor regulatory protein g-8 is involved with the regulation of spontaneous activity and mental condition.2011

    • Author(s)
      Yamazaki M.
    • Organizer
      第8回IBRO
    • Place of Presentation
      イタリア・フィレンツェ
    • Year and Date
      2011-07-17
  • [Presentation] NMDA receptor GluN2B subunit is crucial for channel function, postsynaptic macromolecular organization, and regulation of actin cytoskeleton at hippocampal CA3 synapses.2010

    • Author(s)
      K. Akashi
    • Organizer
      NEUROSCIENCE2010
    • Place of Presentation
      アメリカ・サンディエゴ
    • Year and Date
      2010-11-16
  • [Presentation] Loss of cerebellar granule cell AMPA receptors co0n tributes to ataxia in stargazer mice.2010

    • Author(s)
      M. Yamazaki
    • Organizer
      NEUROSCIENCE2010
    • Place of Presentation
      アメリカ・サンディエゴ
    • Year and Date
      2010-11-14
  • [Presentation] TARPγ2、γ7は小脳AMPA型グルタミン酸受容体の機能的構成成分であるTARPsγ-2 andγ-7 are functional components of cerebellar AMPA receptor.2010

    • Author(s)
      山崎真弥
    • Organizer
      第33回日本神経科学大会第53回日本神経化学会大会合同大会NEURO2010
    • Place of Presentation
      兵庫県神戸市
    • Year and Date
      2010-09-03
  • [Presentation] Transmembrane AMPA receptor regulatory protein gamma-2 and gamma-7 are essential for AMPA receptor complex.2010

    • Author(s)
      Yamazaki M.
    • Organizer
      FENS Forum2010
    • Place of Presentation
      オランダ・アムステルダム
    • Year and Date
      2010-07-06
  • [Presentation] Analysis of the physiological function of cerebellar AMPA receptor subunits using Purkinje cell-specific gene targeting system.2009

    • Author(s)
      M. Abe
    • Organizer
      NEUROSCIENCE2009
    • Place of Presentation
      アメリカシカゴ
    • Year and Date
      2009-10-17
  • [Presentation] Composition of AMPA receptor subunits in the mouse brain ; Quantitative analysis of AMPA receptor subunits.2009

    • Author(s)
      H. Azechi
    • Organizer
      NEUROSCIENCE2009
    • Place of Presentation
      アメリカシカゴ
    • Year and Date
      2009-10-17
  • [Presentation] MADA receptor Glum2B subunit is crucial for channel function and postsynaptic molecular organization at Hippocampal Ca3 synapses.2009

    • Author(s)
      Sakimura, K
    • Organizer
      ISN(国際神経化学会) 22 rd. Biennial Meeting
    • Place of Presentation
      韓国プサン市
    • Year and Date
      2009-08-25
  • [Remarks]

    • URL

      http://www.bri.niigata-u.ac.jp/~cellular/

  • [Patent(Industrial Property Rights)] ES細胞における相同組換え効率を改善する方法2010

    • Inventor(s)
      崎村建司、阿部学、八矢幸大
    • Industrial Property Rights Holder
      新潟大学
    • Industrial Property Number
      国内特許、特願2010-228524
    • Filing Date
      2010-08-10

URL: 

Published: 2013-07-31  

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