2011 Fiscal Year Final Research Report
Control of intranuclear disposition of DNA for gene therapy
| Project/Area Number |
21300174
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | Ehime University |
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Principal Investigator |
KAMIYA Hiroyuki 愛媛大学, 大学院・理工学研究科, 教授 (10204629)
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| Co-Investigator(Kenkyū-buntansha) |
AKITA Hidetaka 北海道大学, 大学院薬学研究院, 准教授 (80344472)
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| Project Period (FY) |
2009 – 2011
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| Keywords | 非ウィルスベクター / 外来DNA / 遺伝子治療 / 核内動態 |
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| Research Abstract |
To achieve efficient and durable transgene expression by controlled intranuclear disposition, we inserted DNA sequences that modulate binding to histones into plasmid DNAs, introduced histone-plasmid DNA complexes into cells, and developed a plasmid system that recruited transcription factors. We found that transgene expression was improved in efficiency and was maintained for a week. Moreover, we revealed that gene correction by tailed duplexes was a useful candidate in gene therapy.
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[Presentation] Gene correction in mouse liver2010
Author(s)
H. Kamiya, M. Uchiyama, J. Piao, Y. Nakatsu, T. Tsuzuki, H. Harashima
Organizer
13th Annual Meeting of the American Society of Gene & Cell Therapy
Place of Presentation
Washington D. C., USA
Year and Date
2010-05-20
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