Development of non-invasive procedures for the diagnosis of chronic hepatitis and non-alcoholic steatohepatitis(NASH) using ultrasound technologies

2011 Fiscal Year Final Research Report

• Project/Area Number: 21300194
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Medical systems
• Research Institution: Hyogo Medical University
• Principal Investigator: IIJIMA Hiroko 兵庫医科大学, 医学部, 教授 (80289066)
• Co-Investigator(Kenkyū-buntansha): NISHIGUCHI Shuhei 兵庫医科大学, 医学部, 教授 (10192246) ; ARII Shigeki 東京医科歯科大学, 医歯(薬)学総合研究科, 教授 (50151171) ; ICHINOSE Shizuko 東京医科歯科大学, 学内共同利用施設等, 助教 (60014156) ; TANAKA Hiroshi 東京医科歯科大学, 大学院・疾患生命科学研究部, 教授 (60155158) ; SATO Tetsuzi 鶴見大学, 歯学部, 教授 (10162447) ; NISHIGAMI Takashi 兵庫医科大学, 医学部, 教授 (70131589)
• Project Period (FY): 2009 – 2011
• Keywords: 超音波医学 / 検査診断システム / 肝臓病学 / 画像診断学

Research Abstract

The diagnostic performance of non-contrast enhanced ultrasound and contrast enhanced ultrasound(CEUS) for chronic hepatitis and non-alcoholic steatohepatitis(NASH) were evaluated. A new diagnostic method for chronic hepatitis was developed using Virtual Touch Tissue Quantification(VTTQ). The diagnosis of NASH by CEUS using Sonazoid was studied. Observations using intravital microscopy on a rat NASH model revealed that the uptake of Sonazoid by Kupffer cells(KCs) did not increase over time, and that was considered due to the decreased phagocytic ability of KCs. The diagnosis of NASH by VTTQ at early stages of the disease suggested some issues that still need to be explored.

Research Products

• [Journal Article] Cost-effectiveness analysis on the surveillance for hepatocellular carcinoma in liver cirrhosis patients using contrast-enhanced ultrasonography (2012)
  • Author(s): Tanaka H, Iijima H, Nouso K, Aoki N, Iwai T, Takashima T, Sakai Y, Aizawa N, Iwata K, Ikeda N, Iwata Y, Enomoto H, Saito M, Imanishi H, Nishiguchi S.
  • Journal Title: Hepatol Res Volume: 3
  • DOI: doi:.1111/j.1872-034X.2011.00936.x.
  • Peer Reviewed
• [Journal Article] 慢性肝疾患の画像診断の進歩-超音波検査は組織診断に代わりうるか- (2011)
  • Author(s): 飯島尋子
  • Journal Title: 日本消化器病学会誌 Volume: 108 Pages: 908-15
  • URL: http://search.jamas.or.jp/index.php
  • Peer Reviewed
• [Journal Article] Acoustic Radiation Force Impulseによる非侵襲的肝線維化診断法の有用性 (2010)
  • Author(s): 飯島尋子, 田中弘教, 吉田昌弘, 池田直人, 山本晃久, 岩田恵典, 榎本平之, 齋藤正紀, 今西宏安, 下村壯治, 中正恵二, 廣田誠一, 辻村亨, 西口修平
  • Journal Title: 肝臓 Volume: 51 Pages: 54-5
  • URL: http://search.jamas.or.jp/index.php
  • Peer Reviewed
• [Journal Article] Crucial role of impaired Kupffer cell phagocytosis on the decreased Sonazoid-enhanced echogenicity in a liver of a nonalchoholic steatohepatitis rat model (2010)
  • Author(s): Yoshikawa S, Iijima H, Saito M, Tanaka H, Imanishi H, Yoshimoto N, Yoshimoto T, Futatsugi-Yumikura S, Nakanishi K, Tsujimura T, Nishigami T, Kudo A, Arii S, Nishiguchi S.
  • Journal Title: Hepatol Res Volume: 40 Pages: 823-31
  • URL: http://www.ncbi.nlm.nih.gov/pubmed/20557367
  • Peer Reviewed
• [Journal Article] 肝疾患の造影超音波診断-その変遷と新しい展開- (2009)
  • Author(s): 飯島尋子
  • Journal Title: 肝臓 Volume: 50 Pages: 105-21
  • URL: http://search.jamas.or.jp/index.php
  • Peer Reviewed
• [Journal Article] Definition of contrast enhancement phases of the liver using a perfluoro-based microbubble agent, perflubutane microbubbles (2009)
  • Author(s): Sasaki S, Iijima H, Moriyasu F, Waki H.
  • Journal Title: Ultrasound Med Biol Volume: 35 Pages: 1819-27
  • URL: http://www.ncbi.nlm.nih.gov/pubmed/19713032
  • Peer Reviewed
• [Presentation] Is ARFI elastography useful for fibrosis evaluation in patients with chronic HCV hepatitis in our clinical practice A multicenter international study (2011)
  • Author(s): Sporea I, Sirli R, Bota S, Tanaka H, Iijima H, Badea R. I, Lupsor M, Fierbinteanu C, Takahashi H, Piscaglia F, D Onofrio M, Popescu A, Danila M.
  • Organizer: The 62th Annual Meeting of the American Association for the Study of Liver Diseases(AASLD2011)
  • Place of Presentation: San Francisco
  • Year and Date: 20111104-09
• [Presentation] VTTQ(Virtual Touch Tissue Quantification) for evaluation of liver tumors (2011)
  • Author(s): Iijima H, Tanaka H, Yoshida M, Iwai T, Takashima T, Sakai Y, Aizawa N, Iwata K, Ikeda N, Iwata Y, Enomoto H, Saito M, Imanishi H, Iimuro Y, Fujimoto J, Nishiguchi S.
  • Organizer: The 62th Annual Meeting of the American Association for the Study of Liver Diseases(AASLD2011)
  • Place of Presentation: San Francisco
  • Year and Date: 20111104-09
• [Presentation] NBNC肝硬変の成因別特色と超音波剪断弾性波(VTTQ)による肝硬度の検討 (2011)
  • Author(s): 青木智子, 飯島尋子, 西口修平
  • Organizer: 第15回日本肝臓学会大会(JDDW2011)
  • Place of Presentation: 福岡
  • Year and Date: 20111020-23
• [Presentation] VTTQ(Virtual Touch Tissue Quantification) for Evaluation of Liver Tumors (2011)
  • Author(s): Iijima H.
  • Organizer: 13th World Congress of the World Federation for Ultrasound in Medicine and Biology(WFUMB2011)
  • Place of Presentation: Vienna
  • Year and Date: 20110826-0831
• [Presentation] Usefulness of virtual touch tissue quantification to diagnose the severity of fibrosis (2011)
  • Author(s): Tanaka H.
  • Organizer: 13th World Congress of the World Federation for Ultrasound in Medicine and Biology(WFUMB2011)
  • Place of Presentation: Vienna
  • Year and Date: 20110826-0831
• [Presentation] VTTQにおける肝硬度規定因子と肝機能の評価 (2011)
  • Author(s): 青木智子, 吉田昌弘, 田中弘教, 廣田誠一, 西上隆之, 岸浩一郎, 西口修平, 飯島尋子
  • Organizer: 日本超音波医学会第84回学術集会
  • Place of Presentation: 東京
  • Year and Date: 20110527-29
• [Presentation] VTTQ法による肝線維化診断における炎症の影響 (2011)
  • Author(s): 吉田昌弘, 田中弘教, 西村純子, 青木智子, 會澤信弘, 西上隆之, 廣田誠一, 藤本治朗, 西口修平, 飯島尋子
  • Organizer: 日本超音波医学会第84回学術集会
  • Place of Presentation: 東京
  • Year and Date: 20110527-29
• [Presentation] 肝表面凹凸のリアルタイム解析による肝線維化の定量化の検討 (2011)
  • Author(s): 神山直久, 飯島尋子, 吉田哲也
  • Organizer: 日本超音波医学会第84回学術集会
  • Place of Presentation: 東京
  • Year and Date: 20110527-29
• [Presentation] Usefulness of VTTQ(Virtual Touch Tissue Quantification) to diagnose fibrosis stage and liver tumors (2011)
  • Author(s): Tanaka H, Iijima H, Yoshida M, Takashima T, Sakai Y, Aizawa N, Ikeda N, Iwata Y, Enomoto H, Saito M, Shimomura S, Imanishi H, Nishigami T, Fujimoto J, Hirota S, Nishiguchi S.
  • Organizer: 46th Annual Meeting of the European Association for the Study of the Liver(EASL2011)
  • Place of Presentation: Berlin
  • Year and Date: 20110330-0403
• [Presentation] Prediction of portal venous invasion by malignant grading system for Hepatocellular carcinoma using Sonazoid contrast enhanced ultrasonography (2010)
  • Author(s): Tanaka H, Iijima H, Higashiura A, Aizawa N, Ikeda N, Enomoto H, Iwata Y, Saito M, Imanishi H, Shimomura S, Hirota S, Nishigami T, Tujimura T, Fujimoto J, Nishiguchi S.
  • Organizer: 12th International symposium on ultrasound contrast imaging
  • Place of Presentation: Tokyo
  • Year and Date: 20101211-12
• [Presentation] Assessment of liver function for patients with chronic HCV infection using the LS ratio by Sonazoid contrast enhanced ultrasonography (2010)
  • Author(s): Yamahira M, Tanaka H, Matsunaga M, Okudaira K, Yoshida M, Higashiura A, Shibata Y, Hashimoto M, Nishiguchi S, Iijima H.
  • Organizer: 12th International symposium on ultrasound contrast imaging
  • Place of Presentation: Tokyo
  • Year and Date: 20101211-12
• [Presentation] Cost-effectiveness analysis on the surveillance for hepatocellular carcinoma in liver cirrhosis patients using contrast-enhanced ultrasonography (2010)
  • Author(s): Tanaka H, Iijima H, Nouso K, Takashima T, Sakai Y, Aizawa N, Ikeda N, Iwata Y, Enomoto H, Saito M, Imanishi H, Shimomura S, Nishiguchi S.
  • Organizer: The 61th Annual Meeting of the American Association for the Study of Liver Diseases(AASLD2010)
  • Place of Presentation: Boston
  • Year and Date: 20101031-1102
• [Presentation] Usefulness of VTTQ(Virtual Touch Tissue Quantification) to diagnose fibrosis and inflammation in chronic hepatitis (2010)
  • Author(s): Iijima H, Tanaka H, Ikeda N, Enomoto H, Iwata Y, Imanishi H, Shimomura S, Fujimoto J, Hirota S, Tsujimura T, Mogushi K, Tanaka H, Nishiguchi S.
  • Organizer: The 61th Annual Meeting of the American Association for the Study of Liver Diseases(AASLD2010)
  • Place of Presentation: Boston
  • Year and Date: 20101031-1102
• [Presentation] Malignant grading system using the contrast enhanced agent"Sonazoid"could predict portal venous invasion by Hepatocellular carcinoma? (2010)
  • Author(s): Iijima H, Tanaka H, Aizawa N, Ikeda N, Enomoto H, Saito M, Imanishi H, Shimomura S, Iimuro Y, Fujimoto J, Nishiguchi S.
  • Organizer: The 61th Annual Meeting of the American Association for the Study of Liver Diseases(AASLD2010)
  • Place of Presentation: Boston
  • Year and Date: 20101031-1102
• [Presentation] What is the reason of the decrease in signal intensity level in Kupffer image of contrast enhanced ultrasonography in non-alcoholic steatohepatitis? (2010)
  • Author(s): Tanaka H, Iijima H, Kudo A, Ichinose S, Yoshimoto S, Yoshikawa S, Nishigami T, Nishiguchi S, Arii S, Wake K.
  • Organizer: 15th International Symposium on Cells of Hepatic Sinusoid Inaugural Meeting for the International Society for Hepatic Sinusoidal Research(ISHSR)
  • Place of Presentation: California
  • Year and Date: 20100829-0901
• [Presentation] びまん性肝疾患のための肝表面凹凸のリアルタイム解析(ワークショップ) (2010)
  • Author(s): 飯島尋子, 神山直久, 吉田哲也
  • Organizer: 日本超音波医学会第83回学術集会
  • Place of Presentation: 京都
  • Year and Date: 20100529-31
• [Presentation] Kupffer細胞の形態からみたNASH発症の要因 (2010)
  • Author(s): 田中弘教, 飯島尋子, 西口修平, 和氣健二郎, 市野瀬志津子, 工藤篤, 石井昭生, 高嶋智之, 坂井良行, 會澤信弘, 山本晃久, 池田直人, 岩田恵典, 榎本平之, 齋藤正紀, 今西宏安, 下村壯治, 有井滋樹
  • Organizer: 第96回日本消化器病学会総会
  • Place of Presentation: 新潟
  • Year and Date: 20100422-24
• [Presentation] Sonazoid造影超音波のKupffer相もおける高音圧画像評価の有用性 (2010)
  • Author(s): 田中弘教, 飯島尋子, 東浦晶子, 楊和典, 岩井孝史, 石井昭生, 高嶋智之, 坂井良行, 會澤信弘, 山本晃久, 池田直人, 岩田恵典, 榎本平之, 齋藤正紀, 今西宏安, 下村壯治, 西口修平
  • Organizer: 第96回日本消化器病学会総会
  • Place of Presentation: 新潟
  • Year and Date: 20100422-24
• [Presentation] 造影超音波のKupffer Phaseからみた非アルコール性脂肪性肝炎の診断と病態 (2009)
  • Author(s): 飯島尋子, 田中弘教, 西口修平
  • Organizer: 第38回日本肝臓学会西部会
  • Place of Presentation: 米子
  • Year and Date: 20091204-05
• [Presentation] Virtual Touch Tissue Quantification法を用いた肝線維化の非襲的評価法 (2009)
  • Author(s): 田中弘教, 飯島尋子, 西口修平
  • Organizer: 第38回日本肝臓学会西部会
  • Place of Presentation: 米子
  • Year and Date: 20091204-05
• [Presentation] Non-invasive diagnosis of liver fibrosis using virtual touch tissue quantification (2009)
  • Author(s): Iijima H, Tanaka H, Saito M, Aizawa N, Yamamoto T, Enomoto H, Iwata Y, Imanishi H, Shimomura S, Yamanaka J, Fujimoto J, Hirota S, Tsujimura T, Nishiguchi S.
  • Organizer: The 60^<th> Annual Meeting of the American Association for the Study of Liver Diseases(AASLD2009)
  • Place of Presentation: Boston
  • Year and Date: 20091031-1103
• [Presentation] 造影超音波検査を用いた輝度解析による慢性肝炎進展度の推定の試み (2009)
  • Author(s): 田中弘教, 飯島尋子, 齋藤正紀, 下村壯治, 今西宏安, 康典利, 榎本平之, 岩田恵典, 山本晃久, 會澤信弘, 坂井良行, 山田大輔, 中村秀次, 廣田誠一, 辻村亨, 西口修平
  • Organizer: 第45回日本肝臓学会総会
  • Place of Presentation: 神戸
  • Year and Date: 20090604-07
• [Presentation] 造影超音波によるピタバスタチンのNASH改善効果に関する検討 (2009)
  • Author(s): 中野宏朗, 飯島尋子, 山田大輔, 坂井良行, 會澤信弘, 山本晃久, 岩田恵典, 田中弘教, 榎本平之, 康典利, 齋藤正紀, 今西宏安, 下村壯治, 中村秀次, 西口修平
  • Organizer: 第45回日本肝臓学会総会
  • Place of Presentation: 神戸
  • Year and Date: 20090604-07
• [Presentation] NASHラットモデル(MCDDラット)におけるクッパー細胞の貪食能の検討 (2009)
  • Author(s): 吉川昌平, 飯島尋子, 石井昭生, 坂井良行, 會澤信弘, 山本晃久, 岩田恵典, 榎本平之, 田中弘教, 今西宏安, 下村壯治, 中村秀次, 西口修平, 工藤篤, 有井滋樹, 和氣健二郎
  • Organizer: 第45回日本肝臓学会総会
  • Place of Presentation: 神戸
  • Year and Date: 20090604-07
• [Presentation] 非アルコール性脂肪性肝炎の病態と造影超音波検査による臨床経過の評価について (2009)
  • Author(s): 齋藤正紀, 飯島尋子, 東浦晶子, 吉川昌平, 田中弘教, 工藤篤, 前山史朗, 有井滋樹, 西口修平
  • Organizer: 日本超音波医学会第82回学術集会
  • Place of Presentation: 東京
  • Year and Date: 20090522-24
• [Presentation] 肝線維化診断におけるARFIの有用性 (2009)
  • Author(s): 吉田昌弘, 田中弘教, 山平正浩, 吉本直喜, 東浦晶子, 柴田陽子, 橋本眞里子, 會澤信弘, 斎藤雅博, 平山秀男, 齋藤正紀, 辻村亨, 廣田誠一, 西口修平, 飯島尋子
  • Organizer: 日本超音波医学会第82回学術集会
  • Place of Presentation: 東京
  • Year and Date: 20090522-24
• [Presentation] ARFIによる肝疾患診断 (2009)
  • Author(s): 飯島尋子, 田中弘教, 西口修平
  • Organizer: (シンポジウム)日本超音波医学会第82回学術集会
  • Place of Presentation: 東京
  • Year and Date: 20090522-24
• [Presentation] びまん性肝疾患の超音波による評価造影超音波検査を用いた慢性肝疾患の肝実質と脾実質の輝度費(LS比)の検討 (2009)
  • Author(s): 山平正浩, 田中弘教, 吉田昌弘, 柴田陽子, 吉本直喜, 東浦晶子, 橋本眞里子, 赤尾憲二, 會澤信弘, 西口修平, 飯島尋子
  • Organizer: 日本超音波医学会第82回学術集会
  • Place of Presentation: 東京
  • Year and Date: 20090522-24
• [Presentation] レボビスト造影超音波検査からみたピタバスタチンのNASH改善効果に関する基礎的検討 (2009)
  • Author(s): 飯島尋子, 田中弘教, 吉川昌平, 齋藤正紀, 中村秀次, 下村壯治, 今西宏安, 康典利, 榎本平之, 岩田恵典, 山本晃久, 會澤信弘, 坂井良行, 山田大輔, 西口修平
  • Organizer: 第95回日本消化器病学会総会
  • Place of Presentation: 札幌
  • Year and Date: 20090507-09
• [Presentation] Sonazoid○Rを用いた慢性肝疾患の肝実質と脾実質の輝度比(LS比)の検討 (2009)
  • Author(s): 山平正浩, 田中弘教, 吉田昌弘, 東浦晶子, 柴田陽子, 橋本眞里子, 辻村亨, 廣田誠一, 西口修平, 飯島尋子
  • Organizer: 日本超音波医学会第36回関西地方会学術集会
  • Place of Presentation: 大阪
  • Year and Date: 2009-10-03
• [Presentation] Sonazoid Kupfferイメージを用いたlow MIとhigh MIによる肝細胞癌診断の試み (2009)
  • Author(s): 東浦晶子, 田中弘教, 山平正浩, 吉田昌弘, 柴田陽子, 橋本眞里子, 藤元治朗, 廣田誠一, 西口修平, 飯島尋子
  • Organizer: 日本超音波医学会第36回関西地方会学術集会
  • Place of Presentation: 大阪
  • Year and Date: 2009-10-03
• [Presentation] 非アルコール性脂肪肝炎(NASH)に対するピタバスチンの改善作用-ラットモデルにおける検討- (2009)
  • Author(s): 吉本直喜, 山平正浩, 吉田昌弘, 東浦晶子, 柴田陽子, 橋本眞里子, 西口修平, 赤尾憲二, 田中弘教, 飯島尋子
  • Organizer: 日本超音波医学会第36回関西地方会学術集会
  • Place of Presentation: 大阪
  • Year and Date: 2009-10-03
• [Presentation] 肝線維化診断におけるAcoustic Radiation Force Impulse(ARFI)の有用性 (2009)
  • Author(s): 吉田昌弘, 田中弘教, 山平正浩, 吉本直喜, 東浦晶子, 柴田陽子, 橋本眞里子, 會澤信弘, 斎藤雅博, 平山秀男, 齋藤正紀, 辻村亨, 廣田誠一, 西口修平, 飯島尋子
  • Organizer: 日本超音波医学会第36回関西地方会学術集会
  • Place of Presentation: 大阪
  • Year and Date: 2009-10-03