Elucidation of microbial cyanotoxin degradation mechanism and use the knowledge for aquatic environment treatment application

2011 Fiscal Year Final Research Report

• Project/Area Number: 21310049
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Environmental technology/Environmental materials
• Research Institution: University of Tsukuba
• Principal Investigator: SUGIURA Norio 筑波大学, 生命環境系, 教授 (10302374)
• Co-Investigator(Kenkyū-buntansha): UTSUMI Motoo 筑波大学, 生命環境系, 准教授 (60323250) ; MASEDA Hideaki 徳島大学, 大学院・ソシオテクノサイエンス研究部, 准教授 (10372343) ; OKANO Kunihiro 秋田県立大学, 生物資源学部, 助教 (30455927) ; SHIMIZU Kazuya 東洋大学, 生命科学部, 助教 (10581613)
• Project Period (FY): 2009 – 2011
• Keywords: 環境修復技術 / 生物膜法

Research Abstract

In the present study, we revealed enzymatic pathway of microbial microcystin degradation in microcystin-degrading bacterium. The abundance of mlrA gene in aquatic environment strongly related to microcystin concentration, thus microcystin-degrading bacteria play a key role in microcystin degradation. Moreover, we confirmed that Adda induce expression of mlrA and mlrB, and cyclic structure of microcystin relate to expression of mlrC. Finally, we have constructed new water treatment apparatus using electrochemical degradation without electrolyte. The apparatus inhibit toxic cyanobacteria growth, but do not inhibit other organism, such as animal plankton and fish.

Research Products

• [Journal Article] Rapid and high efficiency transformation of Sphingomonas and Sphingopyxis by electroporation using frozen cell suspensions (2012)
  • Author(s): Maseda H., Doi Y., Okano K., Sugiura N., Kobayashi M.
  • Journal Title: Journal of Bioindustrial Science Volume: 1 Pages: 1-4
  • Peer Reviewed
• [Journal Article] Investigations into the biodegradation of microcystin-LR mediated by the biofilm in wintertime from a biological treatment facility in a drinking-water treatment plant (2012)
  • Author(s): Li J., Shimizu K., Maseda H., Lu Z., Utsumi M., Zhang Z., and Sugiura N
  • Journal Title: Bioresource Technology Volume: 106 Pages: 27-35
  • Peer Reviewed
• [Journal Article] How microcystin-degrading bacteria express microcystin degradation activity, Lake & Reservoirs (2011)
  • Author(s): Shimizu K., Maseda H., Okano K., Itayama T., Kawauchi Y., Chen R., Utsumi M., Zhang Z., and Sugiura N.
  • Journal Title: Research and Management Volume: 16 Pages: 169-178
  • Peer Reviewed
• [Journal Article] Biodegradation of microcystin by bacterial communities co-existent with flagellate Monas guttula and concurrent succession of the community structures (2011)
  • Author(s): Li J., Shimizu K., Zhou Y., Utsumi M., Sakharkar K. M., Zhang Z., Sun H., and Sugiura N.
  • Journal Title: Journal of Water Supply Volume: 60 Pages: 352-363
  • Peer Reviewed
• [Journal Article] Comparative study for the effects of variable nutrient conditions on the biodegradation of microcystin-LR and concurrent dynamics in microcystin-degrading gene abundance (2011)
  • Author(s): Li J., Shimizu K., Sakharkar K. M., Utsumi M., Zhang Z., and Sugiura N.
  • Journal Title: Bioresource Technology Volume: 102 Pages: 9509-9517
  • Peer Reviewed
• [Journal Article] Dynamics of the functional gene copy number and overall bacterial community during microcystin-LR degradation by a biological treatment facility in a drinking water treatment plant (2011)
  • Author(s): Li J., Shimizu K., Utsumi M., Nakamoto T., Sakharkar M.K., Zhang Z., and Sugiura N.
  • Journal Title: Journal of Bioscience and Bioengineering Volume: 111 Pages: 695-701
  • Peer Reviewed
• [Journal Article] Quantification of microcystin-degrading Bacteria in a Biofilm from a Practical Biological Treatment Facility by Real-time PCR (2010)
  • Author(s): Jimbo Y., Okano K., Shimizu K., Maseda H., Fujimoto N., Utsumi M., and Sugiura N.
  • Journal Title: Journal of Water and Environment Technology Volume: 8 Pages: 193-201
  • Peer Reviewed
• [Journal Article] Characteristics of a microcystin-degrading bacterium under alkaline environmental conditions (2009)
  • Author(s): Okano K., Shimizu K., Kawauchi Y., Maseda H., Utsumi M., Zhang Z, Neilan B., and Sugiura N.
  • Journal Title: Journal of Toxicology
  • DOI: DOI:10.1155/2009/954291
  • Peer Reviewed
• [Presentation] Inhibition on Microcystis aeruginosa growth and microcystins production in oxygen production system using a new electrolysis cell (2012)
  • Author(s): Gao Y., Shimizu K., Utsumi M., Sugiura N
  • Organizer: 第46回日本水環境学会年会
  • Place of Presentation: 東洋大学白山第二キャンパス
  • Year and Date: 2012-03-16
• [Presentation] 生物膜法におけるmicrocystin分解および分解酵素遺伝子の動態解析 (2012)
  • Author(s): 赤迫春菜, 清水和哉, 李潔明, 内海真生, 杉浦則夫
  • Organizer: 第46回日本水環境学会年会
  • Place of Presentation: 東洋大学白山第二キャンパス
  • Year and Date: 2012-03-16
• [Presentation] 電解酸素曝気が藻類の増殖に及ぼす影響評価 (2012)
  • Author(s): 崔海龍, 高宇, 清水和哉, 内海真生, 杉浦則夫
  • Organizer: 第46回日本水環境学会年会
  • Place of Presentation: 東洋大学白山第二キャンパス
  • Year and Date: 2012-03-16
• [Presentation] 日本とタイ水域のおけるmicrocystinの合成および分解に関与する遺伝子(mcy, mlr)の比較解析 (2012)
  • Author(s): 古澤文章, 板山朋聡, 内海真生, 杉浦則夫
  • Organizer: 第46回日本水環境学会年会
  • Place of Presentation: 東洋大学白山第二キャンパス
  • Year and Date: 2012-03-14
• [Presentation] Problems of water supply relevant to Global Warming (2011)
  • Author(s): Sugiura N., Itayama T., Iwami N., Maseda H., and Shimizu K.
  • Organizer: The Fourth International Fisheries Conference
  • Place of Presentation: Maejo University in Thailand
  • Year and Date: 2011-12-01
• [Presentation] Determination of the microcystin-LR degrading genes, mlrAs on biofilm in water purification plant (2011)
  • Author(s): Shimizu K., Maseda H., Okano K., Hiratsuka T., Jimbo Y., Xue Q., Itayama T., Utsumi M., Zhang Z., and Sugiura N.
  • Organizer: The Fourth International Fisheries Conference 2011
  • Place of Presentation: Maejo University in Thailand
  • Year and Date: 2011-12-01
• [Presentation] Dynamics of microcystin production mcy gene and microcystin degradation mlr gene (2011)
  • Author(s): Furusawa F., Shimizu K., Whangchai N., Itayama T., Utsumi M. and Sugiura N.
  • Organizer: The Fourth International Fisheries Conference 2011
  • Place of Presentation: Maejo University in Thailand
  • Year and Date: 2011-12-01
• [Presentation] 生物学的処理法におけるmicrocystin分解活性および分解酵素遺伝子の挙動解析 (2011)
  • Author(s): 赤迫春菜, 清水和哉, 李潔明, 内海真生, 杉浦則夫
  • Organizer: 日本水処理生物学会第48回大会
  • Place of Presentation: 立命館大学びわこ・くさつキャンパス
  • Year and Date: 2011-11-17
• [Presentation] 国内水域とタイ北部水域のmicrocystin産生能の比較解析, 日本水処理生物学会 (2011)
  • Author(s): 古澤文章, 板山朋聡, 岩見徳雄, 清水和哉, Niwooti Whangchai, Chayarat Pleumsumran, Sirapran Fakrajang, Ruenkeaw Praphrute, Redel Gutierrez, Khomsan Ruangdet, Korntip Kannika, 内海真生, 杉浦則夫
  • Organizer: 第48回大会
  • Place of Presentation: 立命館大学びわこ・くさつキャンパス
  • Year and Date: 2011-11-17
• [Presentation] 電解酸素曝気環境下での藻類の増殖特性に関する影響評価 (2011)
  • Author(s): 崔海龍, 高宇, 清水和哉, 内海真生, 杉浦則夫
  • Organizer: 日本水処理生物学会第48回大会
  • Place of Presentation: 立命館大学びわこ・くさつキャンパス
  • Year and Date: 2011-11-17
• [Presentation] ミクロシスチン分解関連タンパク質MlrBの局在と機能解析 (2011)
  • Author(s): 小林宏聡, 清水和哉, 白井昭博, 大政健史, 杉浦則夫, 間世田英明
  • Organizer: 日本水処理生物学会第48回大会
  • Place of Presentation: 立命館大学びわこ・くさつキャンパス
  • Year and Date: 2011-11-17
• [Presentation] 有毒藍藻産生物質ミクロシスチン分解細菌の分解機構の解析 (2011)
  • Author(s): 清水和哉, 間世田英明, 岡野邦宏, 内海真生, 張振亜, 杉浦則夫
  • Organizer: 日本農芸化学会関東支部2011年度大会
  • Place of Presentation: 東洋大学板倉キャンパス
  • Year and Date: 2011-10-15
• [Presentation] Characterization of MlrB involved in degradation of cyanobacterial toxin microcystin LR (2011)
  • Author(s): Kobayashi H., Shimizu K., Zhang Z., Sugiura N., Omasa T., and Maseda H.
  • Organizer: Doctoral Forum of China and the 4th China. Japan Graduate Student Forum
  • Place of Presentation: Chinese geoscience University, Beijing in China
  • Year and Date: 2011-09-25
• [Presentation] Study of biologicalミクロシスチンdegradation at biological treatment facility in a water purification plant, Japan (2011)
  • Author(s): Akasako H., Shimizu K., Li J., Utsumi M., and Sugiura N.
  • Organizer: Doctoral Forum of China and the 4th China. Japan Graduate Student Forum
  • Place of Presentation: Chinese geoscience University, Beijing in China
  • Year and Date: 2011-09-25
• [Presentation] Expression of microcystin degradative activity in microcystin-degrading bacterium (2011)
  • Author(s): Shimizu K., Maseda H., Okano K., Itayama T., Utsumi M., Zhang Z., and Sugiura N.
  • Organizer: International Union of Microbiological Societies 2011 Congress
  • Place of Presentation: Sapporo Convention Center
  • Year and Date: 2011-09-09
• [Presentation] Application of Oxygen Productive Electrode (OPE) to the Toxic Cyanobacteria Inhibition in water treatment (2011)
  • Author(s): Gao Y., Xue Q., Omori K., Shimizu K., Utsumi M., Sugiura N.
  • Organizer: 日本水環境学会
  • Place of Presentation: 北海道大学
  • Year and Date: 2011-03-20
• [Presentation] 水環境生態系に及ぼす電解酸素の影響評価 (2011)
  • Author(s): 大森啓, 高宇, 崔海龍, 清水和哉, 内海真生, 杉浦則夫
  • Organizer: 日本水環境学会
  • Place of Presentation: 北海道大学
  • Year and Date: 2011-03-20
• [Presentation] 生物処理槽におけるミクロシスチン分解能および分解酵素遺伝子の挙動解析 (2011)
  • Author(s): 赤迫春菜, 清水和哉, 李潔明, 内海真生, 杉浦則夫
  • Organizer: 日本水環境学会
  • Place of Presentation: 北海道大学
  • Year and Date: 2011-03-20
• [Presentation] microcystin分解産物の特性解析 (2011)
  • Author(s): 清水和哉, 星麻里恵, 伊藤聡史, 間世田英明, 岡野邦宏, 陳栄志, 内海真生, 張振亜, 杉浦則夫
  • Organizer: 日本水環境学会
  • Place of Presentation: 北海道大学
  • Year and Date: 2011-03-20
• [Presentation] ミクロシスチン分解産物の特性解析 (2010)
  • Author(s): 清水和哉, 星麻里恵, 伊藤聡史, 間世田英明, 岡野邦宏, 陳栄志, 内海真生, 張振亜, 杉浦則夫
  • Organizer: 日本水処理生物学会
  • Place of Presentation: 筑波大学大学院
  • Year and Date: 2010-11-18
• [Presentation] 電解酸素発生装置を用いた純酸素曝気による水質改善手法の検討 (2010)
  • Author(s): 大森啓, 清水和哉, 高宇, 内海真生, 杉浦則夫
  • Organizer: 日本水処理生物学会
  • Place of Presentation: 筑波大学大学院
  • Year and Date: 2010-11-18
• [Presentation] Biodegradation of ミクロシスチンs by the bacterial communities symbiotic with microflagellate Monas guttula (2010)
  • Author(s): Li J., Shimizu K., Utsumi M., Sugiura N
  • Organizer: 日本水処理生物学会
  • Place of Presentation: 筑波大学大学院
  • Year and Date: 2010-11-18
• [Presentation] Application of Oxygen Productive Electrode (OPE) in water treatment (2010)
  • Author(s): Gao Y., Shimizu K., Omori K., Xue Q., Utsumi M., Sugiura N.
  • Organizer: 日本水処理生物学会
  • Place of Presentation: 筑波大学大学院
  • Year and Date: 2010-11-18
• [Presentation] 藍藻由来毒性物質microcystin分解酵素遺伝子の機能解析 (2009)
  • Author(s): 清水和哉, 間世田英明, 岡野邦宏, 内海真生, 杉浦則夫
  • Organizer: 日本水処理生物学会
  • Place of Presentation: 高知県高知市・高知市文化プラザかるぽーと
  • Year and Date: 2009-11-12
• [Presentation] Finding of microcystin-degrading Bacterium and Elucidation of Its Degradation Mechanism, 13th World Lake Conference (2009)
  • Author(s): Shimizu K., Okano K., Maseda H., Kawauchi Y., Utsumi M., Zhang Z. and Sugiura N.
  • Organizer: Key note presentation
  • Place of Presentation: 中華人民共和国, 武漢
  • Year and Date: 2009-11-03