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2011 Fiscal Year Final Research Report

Application of a novel gene-disruption technique based on the suppression of NMD for the T-lymphocyte research

Research Project

  • PDF
Project/Area Number 21310128
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied genomics
Research InstitutionNara Institute of Science and Technology

Principal Investigator

YASUMASA Ishida  奈良先端科学技術大学院大学, バイオサイエンス研究科, 准教授 (10221756)

Project Period (FY) 2009 – 2011
Keywords遺伝子トラップ / マウス / ES細胞 / nonsense-mediated mRNA decay / リンパ球
Research Abstract

In order to improve the efficiency of random insertional mutagenesis, the backbone of an NMD-suppressing poly(A)-trap technique "UPATrap" has been changed from a standard retrovirus to a DNA transposon Tol2. This modification increased the disruption rate of transcriptionally silent genes in mouse embryonic stem(ES) cells and permitted conditional inactivation of trapped genes. In addition, we solved a long-standing problem associated with the transposon vector system by incorporating a mixture of 15 differentially tagged Tol2 transposons. These developments will certainly accelerate the analyses on the nature of T lymphocytes.

  • Research Products

    (6 results)

All 2012 2011 Other

All Journal Article (4 results) (of which Peer Reviewed: 4 results) Presentation (2 results)

  • [Journal Article] CtBP-interacting BTB zinc finger protein(CIBZ) promotes proliferation and G1/S transition in embryonic stem cells via Nanog.2012

    • Author(s)
      Nishii, T., Oikawa, Y., Ishida, Y., Kawaichi, M., and Matsuda, E.
    • Journal Title

      J. Biol. Chem

      Volume: Vol.287 Pages: 12417-12424

    • Peer Reviewed
  • [Journal Article] The methyl-CpG-binding protein CIBZ suppresses myogenic differentiation by directly inhibiting myogenin expression2011

    • Author(s)
      Oikawa, Y., Omori, R., Nishii, T., Ishida, Y., Kawaichi, M., and Matsuda, E.
    • Journal Title

      Cell Res

      Volume: Vol.21 Pages: 1578-1590

    • Peer Reviewed
  • [Journal Article] Evidence that the Upf1-related molecular motor scans the 3'-UTR to ensure mRNA integrity.

    • Author(s)
      Shigeoka, T., Kato, S., Kawaichi, M., and Ishida, Y.
    • Journal Title

      Nucleic Acids Res

      Volume: (in press)

    • Peer Reviewed
  • [Journal Article] Mixture of differentially tagged Tol2 transposons accelerates conditional disruption of a broad spectrum of genes in mouse embryonic stem cells.

    • Author(s)
      Mayasari, N. I., Mukougawa, K., Shigeoka, T., Kawakami, K., Kawaichi, M, and Ishida, Y.
    • Journal Title

      Nucleic Acids Res

      Volume: (in press)

    • Peer Reviewed
  • [Presentation] Mixture of differentially tagged Tol2 transposons accelerates conditional disruption of a broad spectrum of genes in mouse embryonic stem cells.2012

    • Author(s)
      Yasumasa Ishida(石田靖雅)
    • Organizer
      The Company of Biologists Workshop "New Technologies and Applications for Genome Engineering"
    • Place of Presentation
      Steiyning, UK(英国)
    • Year and Date
      2012-03-25
  • [Presentation] 条件的遺伝子改変ES細胞株の量産とデータベース化2011

    • Author(s)
      N. I. Mayasari、田畑海渡、石田靖雅
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      20111213-16

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Published: 2013-07-31  

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