2011 Fiscal Year Final Research Report
Interactome analysis of protein kinases
| Project/Area Number |
21310129
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied genomics
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| Research Institution | Kyoto University (2010-2011)
Keio University (2009) |
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Principal Investigator |
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| Research Collaborator |
SUGIYAMA Naoyuki 慶應義塾大学, 政策メディア研究科, 特任講師 (50545704)
YOSHIHARA Hiroki 慶應義塾大学, 政策メディア研究科, 助教 (90348706)
MASUDA Takeshi 慶應義塾大学, 政策メディア研究科, 研究員 (70383940)
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| Project Period (FY) |
2009 – 2011
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| Keywords | 翻訳後修飾 / リン酸化プロテオーム |
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| Research Abstract |
We profiled recombinant human kinases by using in vitro kinase assay combined with quantitative phosphoproteomics approaches. As a result, 175, 574 kinase-substrate relationships were obtained from the in vitro assay using HeLa cell lysate as the substrate source. Based on the kinase-substrate combination data, we successfully extracted a total of 1, 525 phosphorylation motifs targeted by 303 kinases and classified the kinases to generate a novel phylogenic' kinome' tree based on in vitro substrates instead of the traditional tree based on the sequence homology of kinase domain. Finally, two approaches based on in vitro substrates and phosphorylation motifs were developed to predict kinases which are activated or inactivated by particular stimuli.
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[Journal Article] Detection of Rap1A as a yessotoxin binding protein from blood cell membranes2010
Author(s)
S. Ujihara, T. Oishi, R. Mouri, R. Tamate, K. Konoki, N. Matsumori, M. Murata, Y. Oshima, N. Sugiyama, M. Tomita, and Y. Ishihama
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Journal Title
Bioorg Med Chem Lett
Volume: 20
Pages: 6443-6
DOI
Peer Reviewed
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