2011 Fiscal Year Final Research Report
Development of wavelength-dependent fluorolabel transfer method to functional domain of target protein
| Project/Area Number |
21310138
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Living organism molecular science
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| Research Institution | University of Toyama |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
HATANAKA Yasumaru 富山大学, 大学院・医学薬学研究部(薬学), 教授 (30111181)
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| Project Period (FY) |
2009 – 2011
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| Keywords | 光クロスリンク / ジアジリン / 蛍光ラベル / 光シス.トランス異性化 / 生体分子相互作用解析 |
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| Research Abstract |
Although fluorescence-based visualization has been primarily applied to investigate the behavior of target protein in a cell, there are few methods for fluorescent labeling at the interacting site. Here, a simple and unique construction of a fluorophore at the biding domain of target protein has been achieved by sequential photoreactions using a non-fluorescent ligand molecule. The labeled protein can be used for the fluorescence-based substrate binding analysis
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