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2011 Fiscal Year Final Research Report

Molecular dissection of prenyltransferase family involved in flavonoid functionalization and the enzyme engineering

Research Project

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Project/Area Number 21310141
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Living organism molecular science
Research InstitutionKyoto University

Principal Investigator

YAZAKI Kazufumi  京都大学, 生存圏研究所, 教授 (00191099)

Project Period (FY) 2009 – 2011
Keywordsフラボノイド / プレニル化 / 酵素工学 / 膜蛋白質 / プラスチド局在
Research Abstract

The first isoflavone-specific prenyltransferase SfG6DT and the first chalcone-specific one SfiLDT have been cloned from cultured cells of Sophora flavescens by utilizing flavone-specific prenyltransferase SfN8DT-1. Both have 9 transmembrane alpha-helices and plastid-targeting signal, and by phylogenetic analysis it was turned out that all those flavonoid prenyltransferase belong to prenyltransferase family for vitamin E biosynthesis. Moreover, a new enzyme GmG4DT involved in phytoalexin biosynthesis in soybean has been identified. Beyond Leguminosae, flavonioid prenyltransferases have been isolated in Macaranga tanarius(Euphorbiaceae), as well. By making serial chimeric enzyme between SfN8DT and SfG6DT, a determinant region for substrate recognition could be determined. Furthermore, metabolic engineering using those enzyme genes have been carried out in plant and bacterial hosts.

  • Research Products

    (19 results)

All 2012 2011 2010 2009 Other

All Journal Article (10 results) (of which Peer Reviewed: 10 results) Presentation (7 results) Remarks (1 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Characterization of HlPT-1, amembrane-bound prenyltransferaseresponsible for the biosynthesis of bitter acids in hops, Biochem2012

    • Author(s)
      Tsurumaru, Y., Yazaki, K.
    • Journal Title

      Biophys. Res. Comun.

      Volume: 417 Pages: 393-398

    • DOI

      10.1016/j.bbrc.2011.11.125

    • Peer Reviewed
  • [Journal Article] Metabolic engineering for the production ofprenylated polyphenols in transgenic legume plants using bacterial and plantprenyltransferases2011

    • Author(s)
      Sugiyama, A., (11人略), Yazaki, K.
    • Journal Title

      Metab. Eng.

      Volume: 13 Pages: 629-637

    • DOI

      DOI:10.1016/j.ymben.2011.07.003

    • Peer Reviewed
  • [Journal Article] Molecular characterization of amembrane-bound prenyltransferase specific for isoflavone from Sophora flavescens2011

    • Author(s)
      Sasaki, K., Tsurumaru, Y., Yamamoto, H., Yazaki, K.
    • Journal Title

      J. Biol. Chem.

      Volume: 286 Pages: 24125-24134

    • DOI

      DOI:10.1074/jbc.M111.244426

    • Peer Reviewed
  • [Journal Article] A Tolerance gene for prenylated flavonoidencodes 26S proteasome regulatory subunit in Sophora flavescens2011

    • Author(s)
      Shitan, N., Yazaki, K.
    • Journal Title

      Biosci. Biotech. Biochem.

      Volume: 75 Pages: 982-984

    • DOI

      10.1271/bbb.10066

    • Peer Reviewed
  • [Journal Article] Production of prenylated flavonoids in tomato fruits expressing a prenyltransferasegene from Streptomyces coelicolor A3(2)2011

    • Author(s)
      Koeduka, T., Yazaki, K.
    • Journal Title

      Plant Biol.

      Volume: 13 Pages: 411-145

    • DOI

      DOI:10.1111/j.1438-8677.2010.00409.x

    • Peer Reviewed
  • [Journal Article] Two solanesyl diphosphate synthases with different subcellular localizations and the irrespective physiological roles in Oryzasativa2010

    • Author(s)
      Ohara, K., Sasaki, K., Yazaki, K.
    • Journal Title

      J. Exp. Bot.

      Volume: 61 Pages: 2683-2692

    • DOI

      DOI:10.1093/jxb/erq103

    • Peer Reviewed
  • [Journal Article] Monoterpene engineering in a woody plant Eucalyptus camaldulensis using a limonenesynthase cDNA2010

    • Author(s)
      Ohara, K., Yazaki, K.
    • Journal Title

      Plant Biotech. J.

      Volume: 8 Pages: 28-37

    • DOI

      DOI:10.1111/j.1467-7652.2009.00461.x

    • Peer Reviewed
  • [Journal Article] An aromatic prenyltransferase-like gene HlPT-1preferentially expressed in lupulin glands of hop2010

    • Author(s)
      Tsurumaru, Y., Yazaki, K.
    • Journal Title

      Plant Biotech.

      Volume: 27 Pages: 199-204

    • DOI

      DOI:10.5511/plantbiotechnology.27.199

    • Peer Reviewed
  • [Journal Article] Molecular cloning and characterization of a cDNA for pterocarpan4-dimethylallyltransferase catalyzing the keyprenylation step in the biosynthesis ofglyceollin, a soybean phytoalexin2009

    • Author(s)
      Akashi, T., Sasaki, K., Aoki, T., Ayabe, S., Yazaki, K.
    • Journal Title

      PlantPhysiol.

      Volume: 149 Pages: 683-693

    • DOI

      DOI:10.1104/pp.108.123679

    • Peer Reviewed
  • [Journal Article] Prenylation of aromatic compounds, a keydiversification of plant secondary metabolites2009

    • Author(s)
      Yazaki, K., Sasaki, K., Tsurumaru, Y.
    • Journal Title

      Phytochemistry

      Volume: 70 Pages: 1739-1745

    • DOI

      DOI:10.1016/j.phytochem.2009.08.023

    • Peer Reviewed
  • [Presentation] Flavonoid-specificprenyl transferases, a membrane-bound enzyme family responsible for polyphenol diversity2011

    • Author(s)
      Yazaki, K.
    • Organizer
      50th Phytochemical Society of Noth America
    • Place of Presentation
      KohalaCoast(Hawaii)
    • Year and Date
      2011-11-14
  • [Presentation] 植物の芳香族化合物の高機能化を司るプレニル化酵素2011

    • Author(s)
      矢崎一史
    • Organizer
      第84回日本生化学会大会
    • Place of Presentation
      京都市
    • Year and Date
      2011-09-23
  • [Presentation] Prenyltransferases for flavonoidsin plants and the utilization of the genes to engineer tomato plant2010

    • Author(s)
      Yazaki, K.
    • Organizer
      Crop Functional Genomics
    • Place of Presentation
      Jeju Island(Korea)
    • Year and Date
      20100414-16
  • [Presentation] 機能性低分子としてのプレニル化ポリフェノールと生合成工学2010

    • Author(s)
      矢崎一史
    • Organizer
      第62回日本生物工学会
    • Place of Presentation
      宮崎
    • Year and Date
      2010-10-28
  • [Presentation] 植物の芳香族基質プレニル基転移酵素と機能性植物低分子2010

    • Author(s)
      矢崎一史
    • Organizer
      2010年度日本農芸化学会大会
    • Place of Presentation
      東京
    • Year and Date
      2010-03-31
  • [Presentation] Prenylation of aromatic compounds, a key diversification of plant secondary metabolites2009

    • Author(s)
      Yazaki, K.
    • Organizer
      Colloquium of the Priority Programme. Evolution of Metabolic Diversity.(SPP1152)
    • Place of Presentation
      Freising, Germany
    • Year and Date
      20090403-05
  • [Presentation] 生合成と蓄積に関する植物細胞の役割分担2009

    • Author(s)
      矢崎一史
    • Organizer
      第46回植物化学シンポジウム
    • Place of Presentation
      神戸
    • Year and Date
      2009-12-17
  • [Remarks]

    • URL

      http://www.rish.kyoto-u.ac.jp/W/LPGE/

  • [Patent(Industrial Property Rights)] 新規のプレニル化酵素活性を有するタンパク質とそれをコードする遺伝子2009

    • Inventor(s)
      矢崎一史、梅基直行
    • Industrial Property Rights Holder
      キリンホールディングス株式会社
    • Industrial Property Number
      出願、特願2009-146754
    • Filing Date
      2009-06-19

URL: 

Published: 2013-07-31  

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