2011 Fiscal Year Final Research Report
Search for new anti-mycobacterial substances using established assay system focusing on the environments of infected region, and analysis of target molecule for development of new drug targets
| Project/Area Number |
21310143
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Living organism molecular science
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| Research Institution | Osaka University |
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Principal Investigator |
ARAI Masayoshi 大阪大学, 大学院・薬学研究科, 准教授 (80311231)
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| Co-Investigator(Kenkyū-buntansha) |
KOBAYASHI Motomasa 大阪大学, 大学院・薬学研究科, 教授 (40116033)
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| Project Period (FY) |
2009 – 2011
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| Keywords | 感染症 / 結核 / 活性天然物 / ケミカルバイオロジー / 海綿 / 海洋微生物 |
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| Research Abstract |
To explore new medicinal leads for Tuberculosis, we succeeded in establishment of the screening systems focusing on the environments of Mycobacterium tuberculosis-infected region. Then, we searched the active substances from the extracts of marine organisms and the culture of marine microbe by established bioassay-guided separation. As a result, we isolated halicyclamins, trichoderins and nybomysin as anti-dormant mycobacterial substances. In addition, we succeeded in choosing the candidates of their target molecules by using genomic DNA library and/ or next-generation sequencer. On the other hand, desferrioxamine E was identified as an inhibitor of biofilm formation of Mycobacterium species. We also clarified the action-mechanism of desferrioxamine E as an inhibitor of biofilm formation.
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