2011 Fiscal Year Final Research Report
Amyloid beta-42-mimotope displaying M13 bacteriophage for vaccine vehicle of Alzheimer's disease
| Project/Area Number |
21310144
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Living organism molecular science
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| Research Institution | Kagoshima University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
HASHIGUCHI Syuhei 鹿児島大学, 理工学研究科, 助教 (40295275)
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| Project Period (FY) |
2009 – 2011
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| Keywords | アルツハイマー認知症 / M13ファージ / ワクチン / 自然免疫 / ミミックペプチド / アミロイドベーター |
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| Research Abstract |
Bacteriophage evokes strong antibody production by means of innate immunity. Recently, we have established B6 mimotope peptide(B6-C15) which mimics the structure of amyloid・42(A・) fibril using human single-chain Fv(B6) specific to A・42 fibril. This mimotope peptide binds to A・42 oligomers and inhibits formation of A・42 fibril in vitro. We investigated whether M13 bacteriophage displaying B6-mimotope peptide(B6-C15-phage) could produce A・42 fibril specific antibody response in mice. When mice were immunized i. p. with B6-C15-phage in PBS solution, they produced anti-A・42 fibril specific IgG response in two weeks. This finding suggested a promising vaccination strategy for immunotherapy of Alzheimer's disease without the involvement of A・42-specific T cell immunity(BBRC 402 : 19-22, 2010, and J. Neuroimmunol. 236 : 27-38, 2011).
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[Journal Article] Direct evidence of generation and accumulation ofβ-sheet-rich prion protein in scrapie-infected neuroblastoma cells with a human IgG1 antibody specific for the b-form prion protein2012
Author(s)
Toshiya Kubota, Yuta Hamazoe, Shuhei Hashiguchi, Daisuke Ishibashi, Kazuyuki Akasaka, Noriyuki Nishida, Shigeru Katamine, Suehiro Sakaguchi, Ryota Kuroki, Toshihiro Nakashima, and Kazuhisa Sugimura
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Journal Title
J. Biol. Chem
Volume: 287
Pages: 14023-14039
URL
Peer Reviewed
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[Journal Article] A mimotope peptide of Aβ42 fibril-specific antibodies with Aβ42 fibrillation inhibitory activity induces anti-Aβ42 conformer antibody response by a displayed form on an M13 phage in mice2011
Author(s)
Koichi Tanaka, Masaaki Nishimura, Yuya Yamaguchi, Shuhei Hashiguchi, Sho Takiguchi, Makoto Yamaguchi, Haruna Tahara, Takuma Gotanda, Risa Abe, Yuji Ito, Kazuhisa Sugimura
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Journal Title
J. Neuroimmunology
Volume: 236
Pages: 27-38
URL
Peer Reviewed
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