2012 Fiscal Year Final Research Report
ANALYSIS OF DYNAMIC BEHAVIOR OF RNA USING LIGHT-UP RNA MODULE AS A TAG
| Project/Area Number |
21350091
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Chemistry related to living body
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| Research Institution | Doshisha University |
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Principal Investigator |
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| Project Period (FY) |
2009 – 2012
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| Keywords | 転写 / RNA / 蛍光 / 蛍光モニタリング / BODIPY / アプタマー / トブラマイシン / 融合遺伝子 |
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| Research Abstract |
The fluorescence intensity of tobramycin-bearing phenyl-BODIPY as a probe increases upon interaction with tobramycin-aptamer, an RNA sequence that specifically binds to tobramycin. Transcription of a target gene (tRNA in this case) containing the aptamer sequence downstream thereof affords a fused (target-aptamer) RNA, the aptamer component of which can be detected by enhanced fluorescence, although no big, in the presence of the probe. In this way, the transcription of the target gene can be fluorescence monitored.
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Research Products
(15 results)
