Creation and Structure-Function Control of Photo-Reactive Proteins and Peptides

2012 Fiscal Year Final Research Report

• Project/Area Number: 21350095
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Chemistry related to living body
• Research Institution: Nara Institute of Science and Technology
• Principal Investigator: HIROTA Shun 奈良先端科学技術大学院大学, 物質創成科学研究科, 教授 (90283457)
• Co-Investigator(Kenkyū-buntansha): NAGAO Satoshi 奈良先端科学技術大学院大学, 物質創成科学研究科, 助教 (30452535)
• Project Period (FY): 2009 – 2012
• Keywords: 光応答性 / タンパク質 / ペプチド / 構造機能相関 / 機能制御

Research Abstract

Control of the structure and function of proteins and peptides with high space and time resolution will initiate new technologies, which include living body measurements and medical treatment. In this research, photo-reactive proteins and peptides were constructed, and photo-control of the structure and function of these biologically -related molecules was performed. The structure-function analysis of the protein with the use of its photoactivity , fixation of a photoactive ligand on a gold surface and regulation of its metal ion binding, and control of DNA cleavage with a photoactive ligand were performed.

Research Products

• [Journal Article] Photosensitivity of the Ni-A State of [NiFe] Hydrogenase from Desulfovibrio vulgaris Miyazaki F with Visible Light (2013)
  • Author(s): H. Osuka, Y. Shomura, H. Komori, N.Shibata, S. Nagao, Y. Higuchi, S. Hirota
  • Journal Title: Biochem.Biophys.Res.Commun Volume: 430 Pages: 284-288
  • Peer Reviewed
• [Journal Article] DNA Cleavage by the Photocontrolled Cooperation of ZnII Centers in an Azobenzene-Linked Dizinc Complex (2011)
  • Author(s): A. Panja, T. Matsuo, S. Nagao, S. Hirota
  • Journal Title: Inorg. Chem Volume: 50 Pages: 11437-11445
  • Peer Reviewed
• [Journal Article] Efficient Reduction of Cys110 Thiyl Radical by Glutathione in Human Myoglobin (2011)
  • Author(s): S. Nagao, O. Asami, H. Yasui, S. Hirota
  • Journal Title: Biochim. Biophys. Acta Proteins and Proteomics Volume: 1814 Pages: 480-486
• [Journal Article] Cytochrome cPolymerization by Successive Domain Swapping at the C-Terminal Helix (2010)
  • Author(s): S. Hirota, Y. Hattori, S. Nagao, M. Taketa, H. Komori, H. Kamikubo, Z. Wang, I. Takahashi, S. Negi, Y. Sugiura, M. Kataoka, Y. Higuchi
  • Journal Title: Proc. Natl. Acad. Sci. USA Volume: 107 Pages: 12854-12859
  • Peer Reviewed
• [Journal Article] Structural Basis of the Lactate-Dependent Allosteric Regulation of Oxygen Binding in Arthropod Hemocyanin (2010)
  • Author(s): S. Hirota, N.Tanaka, I. Micetic, P. Di Muro,S. Nagao, H. Kitagishi, K. Kano, R. S. Magliozzo, J. Peisach, M. Beltramini, L. Bubacco
  • Journal Title: J. Biol. Chem Volume: 285 Pages: 19338-19345
  • Peer Reviewed
• [Journal Article] Regulating Copper-Binding Affinity with Photoisomerizable Azobenzene Ligand by Construction of a Self-Assembled Monolayer (2009)
  • Author(s): I. Takahashi, Y. Honda, S. Hirota
  • Journal Title: Angew. Chem. Int. Ed Volume: 48 Pages: 6065-6068
  • Peer Reviewed
• [Presentation] Structural Changes of Metalloproteinsand Metal-Peptide Complexes (2012)
  • Author(s): Shun Hirota
  • Organizer: Third Dalton Transactions International Symposium: Bioinorganic Chemistry
  • Place of Presentation: Suita
  • Year and Date: 2012-11-14
• [Presentation] Investigation and Regulation of Protein and Peptide Structural Changes (2011)
  • Author(s): Shun Hirota
  • Organizer: Japanese-German Scientific Cooperation in the 21st Century: Biocatalysts for Future Feedstock Utilization
  • Place of Presentation: Nara
  • Year and Date: 2011-04-26
• [Presentation] 光異性化可能なアゾベンゼン配位子の表面固定による銅イオンの結合制御 (2010)
  • Author(s): 高橋勇雄、本田裕一郎、廣田俊、長谷川美貴
  • Organizer: 第22回配位化合物の光化学討論会
  • Place of Presentation: 富山市
  • Year and Date: 20100803-05
• [Presentation] Protein and Peptide Structural Changes: Protein Aggregation and PhotoactiveBiomaterials (2010)
  • Author(s): Shun Hirota
  • Organizer: Nanotechnology and Medical Sciences(ICNMS-2010)
  • Place of Presentation: Kolhapur, India
  • Year and Date: 2010-10-22
• [Presentation] Photoregulation of Copper-Binding Affinity to the Azobenzene Ligand on Au Surface (2010)
  • Author(s): Isao Takahashi, Yuichiro Honda, Shun Hirota, Miki Hasegawa
  • Organizer: International Conference on Nanoscopic Colloid and Surface Science
  • Place of Presentation: Chiba
  • Year and Date: 2010-09-20
• [Presentation] Peptides, and Inorganic Compounds Incorporated with Photo-Triggering and Photo-Regulating Properties (2010)
  • Author(s): Shun Hirota, Isao Takahashi, Halan Prakash, Satoshi Nagao, Proteins
  • Organizer: 5th International Symposium on Macrocyclic and Supramolecular Chemistry (ISMSC 2010)
  • Place of Presentation: Nara, Japan
  • Year and Date: 2010-06-09
• [Presentation] Photoregulated Metal Binding to the Azobenzene Ligand on Au Surface (2009)
  • Author(s): Isao Takahashi, Yuichiro Honda, Shun Hirota
  • Organizer: 14th International Conference on Biological Inorganic Chemistry (ICBIC14)
  • Place of Presentation: Nagoya
  • Year and Date: 20090725-30
• [Presentation] Bridging Ligand at the Active Site of Oxidized [NiFe] Hydrogenase (2009)
  • Author(s): Hisao Osuka, Shun Hirota, Shin-ichi Terawaki, Yasuhito Shomura, Hirofumi Komori, Naoki Shibata, Yoshiki Higuchi
  • Organizer: 14th International Conference on Biological Inorganic Chemistry (ICBIC14)
  • Place of Presentation: Nagoya
  • Year and Date: 20090725-30
• [Presentation] Effect of Cysteine Residue on Reaction of HumanMyoglobin with Hydrogen Peroxide (2009)
  • Author(s): Satoshi Nagao, Osamu Asami, Shun Hirota
  • Organizer: 14th International Conference on Biological Inorganic Chemistry (ICBIC14)
  • Place of Presentation: Nagoya
  • Year and Date: 20090725-30
• [Presentation] Structural Origin for the Bohr Effect in Arthropod Hemocyanin (2009)
  • Author(s): Shun Hirota, Takumi Kawahara, Mariano Beltramini, Paolo Di Muro, Richard S. Magliozzo, Jack Peisach, Linda S. Powers,Naoki Tanaka, Satoshi Nagao, Luigi Bubacco
  • Organizer: 14th International Conference on Biological Inorganic Chemistry (ICBIC14)
  • Place of Presentation: Nagoya
  • Year and Date: 20090725-30
• [Presentation] Investigation and Regulation of Proteinand Peptide Structural Changes (2009)
  • Author(s): Shun Hirota
  • Organizer: International Symposium on Protein Structures: Stability
  • Place of Presentation: Interaction and Dynamics, Nara
  • Year and Date: 2009-12-18
• [Presentation] Proteins, Peptides and Inorganic Compounds Incorporated with Photo-Triggering and Photo-Regulating Properties (2009)
  • Author(s): Shun Hirota
  • Organizer: The 9th GIST/NAIST JointSymposium on Advanced Materials
  • Place of Presentation: Gwangju, Korea
  • Year and Date: 2009-11-18
• [Presentation] Peptides and Inorganic Compounds Incorporated with Photo-Triggering and Photo-Regulating Properties (2009)
  • Author(s): Shun Hirota, Isao Takahashi, Halan Prakash, Yuichiro Honda, Satoshi Nagao, Proteins
  • Organizer: The 1st NCTU-NAIST Workshop on "Molecular/Nano Science" 2009
  • Place of Presentation: Hsinchu, Taiwan
  • Year and Date: 2009-11-13
• [Presentation] [NiFe] ヒドロゲナーゼのX線結晶構造解析と赤外分光測定(X-ray crystallographic and FT-IR studies on [NiFe] hydrogenase) (2009)
  • Author(s): Hisao Osuka, Shin-ichi Terawaki, Yasuhito Shomura, Hirofumi Komori, Naoki Shibata, Shun Hirota, Yoshiki Higuchi
  • Organizer: The 47th Annual Meeting of the Biophysical Society of Japan
  • Place of Presentation: Tokushima
  • Year and Date: 2009-10-30
• [Presentation] アゾベンゼン配位子の表面固定を利用した銅イオンの結合制御 (2009)
  • Author(s): 高橋勇雄、本田裕一朗、廣田俊
  • Organizer: 第59回錯体化学討論会
  • Place of Presentation: 長崎市
  • Year and Date: 2009-09-25
• [Presentation] 節足動物由来ヘモシアニンの酸素結合挙動に対するpHおよび乳酸の影響 (2009)
  • Author(s): 廣田俊、田中直輝、長尾聡、北岸宏亮、加納航冶、I. Micetic、M. Bertamini、L.Bubacco
  • Organizer: 第36回生体分子科学討論会
  • Place of Presentation: 札幌市
  • Year and Date: 2009-06-19
• [Book] 光で生体関連分子の構造を制御する、現代化学10月号 (2010)
  • Author(s): 廣田俊
  • Total Pages: 36-40
  • Publisher: 東京化学同人
• [Remarks]
  • URL: http://mswebs.naist.jp/LABs/hirota/index.html
• [Patent(Industrial Property Rights)] リンカーを有するアゾ化合物及び当該アゾ化合物を用いた金属イオン回収方法 (2013)
  • Inventor(s): 廣田俊、高橋勇雄、本田裕一朗
  • Industrial Property Rights Holder: 奈良先端科学技術大学院大学
  • Industrial Property Number: 特願5211362
  • Acquisition Date: 2013-03-08
• [Patent(Industrial Property Rights)] アゾペプチド複合体 (2013)
  • Inventor(s): 廣田俊、ハランプラカシュ
  • Industrial Property Rights Holder: 奈良先端科学技術大学院大学
  • Industrial Property Number: 特願5190594
  • Acquisition Date: 2013-02-08
• [Patent(Industrial Property Rights)] 光制御ペプチド及び光制御ペプチドを用いたペプチド-蛋白質複合体形成の制御方法 (2010)
  • Inventor(s): 廣田俊、濱崎勇二
  • Industrial Property Rights Holder: 奈良先端科学技術大学院大学
  • Industrial Property Number: 特願4645650
  • Acquisition Date: 2010-12-17