2011 Fiscal Year Final Research Report
Structural Chemistry on Information Transduction through Allosteric Effects in Heme Proteins
| Project/Area Number |
21350098
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Chemistry related to living body
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| Research Institution | University of Hyogo |
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Principal Investigator |
KITAGAWA Teizo 兵庫県立大学, 大学院・生命理学研究科, 教授 (40029955)
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| Co-Investigator(Kenkyū-buntansha) |
OGURA Takashi 兵庫県立大学, 大学院・生命理学研究科, 教授 (70183770)
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| Project Period (FY) |
2009 – 2011
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| Keywords | 生物無機化学 / 振動分光学 / アロステリック効果 / ガスセンサー蛋白 / ヘモグロビン / 情報伝達 |
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| Research Abstract |
Structural chemistry involved in allosteric effects of heme proteins was investigated with visible and ultraviolet resonance Raman spectroscopy. About hemoglobin as a fundamental molecule, a quaternary structure of half-ligated state was quantitatively determined on the basis of inter-subunit interactions. As applications, information transduction pathway and its dynamics were elucidated with two oxygen sensor proteins, DOS and HemAT, and structural effects of effector binding was revelaed for an NO sensor protein, sGC. For respiration enzyme, proton pumping mechanism of a mammalian cytochrome c oxidase was examined.
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