X線マイクロビームを用いた神経細胞回路作成技術の開発

2011 Fiscal Year Annual Research Report

• Project/Area Number: 21360041
• Research Institution: Osaka University
• Principal Investigator: 飯田 敏行 大阪大学, 大学院・工学研究科, 教授 (60115988)
• Co-Investigator(Kenkyū-buntansha): 加藤 祐史 大阪大学, 大学院・工学研究科, 准教授 (40224547) ; 佐藤 文信 大阪大学, 大学院・工学研究科, 助教 (40332746)
• Keywords: 培養細胞パターニング / X線マイクロビーム / 放射線照射効果 / 神経細胞培養 / 分化誘導

Research Abstract

本研究課題"X線マイクロビームを用いた神経細胞回路作成技術の開発"では、X線マイクロビーム照射技術と半導体プロセス技術を細胞工学に取り入れて、神経培養細胞が任意のパターンに人工配列される神経回路(神経細胞チップ)の作成技術を開発した。
半導体プロセス技術によって培養基板上に細胞外マトリックスをパターン加工し、細胞は培養中において、パターン状にしたがって接着する。神経細胞チップ上に配置される培養細胞には、PC12細胞を用いた。通常、PC12細胞は、NGF成長因子の投与によって神経突起が成長する。また、強いX線や電磁波によっても、NGF成長因子の投与なしで神経突起を誘発することが報告されている。そこで、X線照射誘導によるPC12細胞の分化について実験を行った。PC12細胞のX線照射には、ビーム径10μmのX線マイクロビーム照射装置を用いて、細胞チップ上のPC12細胞を最大吸収線量20Gyまで照射し、インキュベータで10日間培養し、1日ごとに細胞の形態を観察した。その結果、X線によって分化誘導されるPC12細胞の効率は、吸収線量の増加とともに高くなり、15Gy付近で最大となって、その分化率は約14%であった。ただし、X線照射によって分化した細胞の神経突起は、NGFの投与にくらべて、小さいことが特徴である。
製作された細胞チップ上の神経細胞は、カルシウムイオン測定用の蛍光プローブ(Fura 2、Fluo 3)と刺激用微小電極を用いてその応答特性を調べた。通常培養条件のPC12細胞と比較して、大きな違いが見られないため、細胞チップ上のPC12細胞の培養に成功していることが確かめられた。

Research Products

• [Journal Article] Dependence of ion beam current on position of mobile plate tuner in multi-frequencies microwaves electron cyclotron resonance ion source (2012)
  • Author(s): Kurisu, Y., Kiriyama, R., Takenaka, T., Nozaki, D., Sato, F., Kato, Y., Iida, T.
  • Journal Title: Review of Scientific Instruments Volume: 83 Pages: 02A310
  • DOI: 10.1063/1.3662016
  • Peer Reviewed
• [Journal Article] Profiles of ion beams and plasma parameters on a multi frequencies microwaves large bore electron cyclotron resonance ion source with permanent magnets (2012)
  • Author(s): Kato, Y., Sakamoto, N., Kiriyama, R., Takenaka, T., Kurisu, Y., Nozaki, D., Sato, F., Iida, T.
  • Journal Title: Review of Scientific Instruments Volume: 83 Pages: 02A317
  • DOI: 10.1063/1.3666184
  • Peer Reviewed
• [Journal Article] Improvement of efficiency and temperature control of induction heating vapor source on electron cyclotron resonance ion source (2012)
  • Author(s): Takenaka, T., Kiriyama, R., Muramatsu, M., Kitagawa, A., Uchida, T., Kurisu, Y., Nozaki, D., Yano, K., Yoshida, Y., Sato, F., Kato, Y., Iida, T.
  • Journal Title: Review of Scientific Instruments Volume: 83 Pages: 02A327
  • DOI: 10.1063/1.3669798
  • Peer Reviewed
• [Journal Article] Electron cyclotron resonance plasma production by using pulse mode microwaves and dependences of ion beam current and plasma parameters on the pulse condition (2012)
  • Author(s): Kiriyama, R., Takenaka, T., Kurisu, Y., Nozaki, D., Sato, F., Kato, Y., Iida, T.
  • Journal Title: Review of Scientific Instruments Volume: 83 Pages: 02A324
  • DOI: 10.1063/1.3669792
  • Peer Reviewed
• [Journal Article] Microscopic dose measurement with thin radiophotoluminescence glass plate (2011)
  • Author(s): Maki, D., Nagai, T., Sato, F., Kato, Y., Yamamoto, T., Iida, T.
  • Journal Title: Radiation Measurements Volume: 46 Pages: 1543-1546
  • DOI: 10.1016/j.radmeas.2011.05.051
  • Peer Reviewed
• [Journal Article] Development of neutron-sensitive glass dosimeter containing isotopically enriched boron (2011)
  • Author(s): Maki, D., Sato, F., Murata, I., Kato, Y., Tanimura, Y., Yamamoto, T., Iida, T.
  • Journal Title: Radiation Measurements Volume: 46 Pages: 1484-1487
  • DOI: 10.1016/j.radmeas.2011.06.068
  • Peer Reviewed
• [Journal Article] Development of photon counting system with FPGA for precise measurement of radiophotoluminescence (2011)
  • Author(s): Ihara, Y., Ishii, T., Nagai, T., Sato, F., Kato, Y., Yamamoto, T., Iida, T.
  • Journal Title: Radiation Measurements Volume: 46 Pages: 1574-1577
  • DOI: 10.1016/j.radmeas.2011.05.056
  • Peer Reviewed
• [Journal Article] The recent investigations of radiophotoluminescence and its application (2011)
  • Author(s): Yamamoto, T., Maki, D., Sato, F., Miyamoto, Y., Nanto, H., Iida, T.
  • Journal Title: Radiation Measurements Volume: 46 Pages: 1554-1559
  • DOI: 10.1016/j.radmeas.2011.04.038
  • Peer Reviewed
• [Journal Article] Performance analysis of fusion nuclear-data benchmark experiments for light to heavy materials in MeV energy region with a neutron spectrum shifter (2011)
  • Author(s): Murata, I., Ohta, M., Miyamaru, H., Kondo, K., Yoshida, S., Iida, T., Ochiai, K., Konno, C.
  • Journal Title: Journal of Nuclear Materials Volume: 417 Pages: 1127-1130
  • DOI: 10.1016/j.jnucmat.2010.12.299
  • Peer Reviewed
• [Journal Article] Characterization of Er_2O_3 ceramic coatings by luminescence measurements (2011)
  • Author(s): Tanaka, T., Yoshino, M., Hishinuma, Y., Zhang, D., Kada, W., Sato, F., Iida, T., Nagasaki, T., Muroga, T.
  • Journal Title: Journal of Nuclear Materials Volume: 417 Pages: 794-797
  • DOI: 10.1016/j.jnucmat.2010.12.162
  • Peer Reviewed
• [Journal Article] Development of thermal neutron-sensitive glass dosemeter containing lithium (2011)
  • Author(s): Maki, D., Kobayashi, H., Sato, F., Murata, I., Kato, Y., Tanaka, T., Yamamoto, T., Iida, T.
  • Journal Title: Radiation Protection Dosimetry Volume: 144 Pages: 226-230
  • DOI: 10.1093/rpd/ncq552
  • Peer Reviewed
• [Journal Article] Development of confocal laser microscope system for examination of microscopic characteristics of radiophotoluminescence glass dosemeters (2011)
  • Author(s): Maki, D., Ishii, T., Sato, F., Kato, Y., Yamamoto, T., Iida, T.
  • Journal Title: Radiation Protection Dosimetry Volume: 144 Pages: 222-225
  • DOI: 10.1093/rpd/ncq563
  • Peer Reviewed
• [Presentation] Measurement of radioactive cesium distribution in soil by use of radiophotoluminescence glass dosimeter covered with thin polyethylene film (2011)
  • Author(s): N.ZUSHI, Y.IKEDA, F.SATO, Y.KATO, K.SHIMIZU, N.SATO, T.YAMAMOTO, T.IIDA
  • Organizer: The 7th International Workshop on Individual Monitoring of Ionizing radiation
  • Place of Presentation: Oarai, Ibaraki, Japan
  • Year and Date: 20111203-20111205
• [Presentation] Development of a bead-type radiophotoluminescence glass dosimeter applicable to various purposes (2011)
  • Author(s): Y.TOYOTA, F.SATO, I.MURATA, Y.KATO, T.IIDA
  • Organizer: The 7th International Workshop on Individual Monitoring of Ionizing radiation
  • Place of Presentation: Oarai, Ibaraki, Japan
  • Year and Date: 20111203-20111205
• [Presentation] Collaboration research on new glass dosimeters and their applications between Osaka University and Chiyoda Technol Oarai research center (2011)
  • Author(s): T.IIDA, F.SATO, T.YAMAMOTO
  • Organizer: The 7th International Workshop on Individual Monitoring of Ionizing radiation
  • Place of Presentation: Oarai, Ibaraki, Japan(招待講演)
  • Year and Date: 20111203-20111205
• [Presentation] Effect of air condition on alpha-radioactivity measurement by air flow pulse ionization chamber (2011)
  • Author(s): Naoki ZUSHI, Yohei IHARA, Keisuke KABASHIMA, Fuminobu SATO, Yushi KATO, Toshiyuki IIDA,
  • Organizer: Sixth International Symposium on Radiation Safety and Detection Technology
  • Place of Presentation: Langkawi, Malaysia
  • Year and Date: 20110712-20110714
• [Remarks]
  • URL: http://fusion.eie.eng.osaka-u.ac.jp