2011 Fiscal Year Final Research Report
Characteristics of Cell Damage and Death under High Temperature Condition and Its Phenomenological Mathematical Model
| Project/Area Number |
21360098
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Thermal engineering
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| Research Institution | Kyushu Institute of Technology |
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Principal Investigator |
ISHIGURO Hiroshi 九州工業大学, 大学院・生命体工学研究科, 教授 (30176177)
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| Project Period (FY) |
2009 – 2011
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| Keywords | 生体熱工学 / 高温ストレス / 温熱ストレス / 細胞損傷 / 死滅モード / 数学的モデル化 / モデル定数 / 温度依存性 |
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| Research Abstract |
Mathematical modeling to characterize high-temperature stress-induced cell damage and death was developed. For four kinds of models with reaction kinetic formulation, a parameter study of the rate constants of cell damage and death in the models was performed to analyze the model' s characteristics. An experiment was also performed to evaluate the time-series changes in the numbers of live and dead cells. The rate constants and the optimum reaction formula were determined by inverse problem analysis on the basis of experimental data of cell viability. Temperature dependency of rate constants was expressed by an Arrhenius' equation. The results show that the cell damage and death can be described well by the reaction kinetic model.
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