Liquid-phase detection of biological targets with magnetic markers

2011 Fiscal Year Final Research Report

• Project/Area Number: 21360199
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Measurement engineering
• Research Institution: Kyushu University
• Principal Investigator: ENPUKU Keiji 九州大学, 超伝導システム科学研究センター, 教授 (20150493)
• Co-Investigator(Kenkyū-buntansha): INOUE Masayoshi 九州大学, システム情報科学研究院, 准教授 (80346824) ; KUMA Hiroyuki 長崎国際大学, 薬学部, 講師 (40435136) ; YOSHISA Takashi 九州大学, システム情報科学研究院, 助教 (30380588) ; NOZAKI Yukio 九州大学, システム情報科学研究院, 助教 (30304760)
• Project Period (FY): 2009 – 2011
• Keywords: 免疫検査 / 磁気マーカー / SQUIDセンサ / 磁気ナノ粒子 / ブラウン運動 / バイオ検査機器

Research Abstract

We have developed a liquid-phase immunoassay technique using Brownian relaxation of magnetic markers. First, we quantitatively characterized the properties of the magnetic markers in order to find the markers suitable for the application. Next, we developed a detection method using large polymer beads to fix the biological targets, where bound and free markers were magnetically distinguished using the difference in their Brownian relaxation. Therefore, we can omit a time consuming washing process called bound/free separation. We also developed a detection system using high Tc SQUID sensor, and demonstrated the high sensitivity detection of biological targets called biotins.

Research Products

• [Journal Article] Characterization of magnetic markers for liquid-phase immunoassays using Brownian relaxation (2012)
  • Author(s): K. Enpuku, H. Watanabe, Y. Higuchi, T. Yoshida, H. Kuma, N. Hamasaki, M. Mitsunaga, H. Kanzaki, and A. Kandori.
  • Journal Title: Jpn. J. Appl. Phys Volume: 51 Pages: 023002
  • Peer Reviewed
• [Journal Article] Liquid-phase immunoassays using Brownian relaxation of magnetic markers (2011)
  • Author(s): A. K. Bhuiya, T. Mitake, M. Asai, T. Ito, S. Chosokabe, T. Yoshida, K. Enpuku and A. Kandori
  • Journal Title: IEEE Trans. Magn Volume: 47 Pages: 2867
  • Peer Reviewed
• [Journal Article] Liquid phase immunoassays utilizing magnetic markers and SQUID magnetometer (2010)
  • Author(s): H. Kuma, H. Oyamada, A. Tsukamoto, T. Mizoguchi, A. Kandori, Y. Sugiura, K. Yoshinaga, K. Enpuku and N. Hamasaki.
  • Journal Title: Clin Chem Lab Med Volume: 48 Pages: 1263
  • Peer Reviewed
• [Journal Article] ac susceptibility measurement of magnetic markers in suspension for liquid phase immunoassay (2010)
  • Author(s): K. Enpuku, Y. Tamai, T. Mitake, T. Yoshida and M. Matsuo.
  • Journal Title: J. Appl. Phys Volume: 108 Pages: 034701
  • Peer Reviewed
• [Journal Article] Fast detection of biological targets with magnetic marker andS QUID (2009)
  • Author(s): K. Enpuku, H. Tokumitsu, Y. Sugimoto, H. Kuma, N. Hamasaki, A. Tsukamoto, T. Mizoguchi, A. Kandori, K. Yoshinaga, H. Kanzaki and N. Usuki.
  • Journal Title: IEEE Trans. Appl. Supercond Volume: 19 Pages: 844
  • Peer Reviewed
• [Journal Article] Development of a SQUID system using field reversal for rapid detecting Bacteria (2009)
  • Author(s): A. Tsukamoto, T. Mizoguchi, A. Kandori, H. Kuma, N. Hamasaki, H. Kanzaki, N. Usuki, K. Yoshinaga and K. Enpuku.
  • Journal Title: IEEE Trans. Appl. Supercond Volume: 19 Pages: 853
  • Peer Reviewed
• [Presentation] Diagnostics and Immunoassays-Magnetic-marker Based SQUID Biosenso (2011)
  • Author(s): K. Enpuku.
  • Organizer: Superconductivity Centinual Conf
  • Place of Presentation: Haag(Netherlands)
  • Year and Date: 2011-09-20
• [Presentation] Liquid-phase immunoassays using Brownian relaxation of magnetic markers", Int. Magn (2011)
  • Author(s): AK. Bhuiya, T. Mitake, M. Asai, T. Ito, S. Chosakabe, T. Yoshida, and K. Enpuku.
  • Organizer: Conf(INTERMAG 2011)
  • Place of Presentation: Taipei(Taiwan)
  • Year and Date: 2011-04-26
• [Presentation] Liquid-phase detection of biological targets with magnetic marker and SQUID (2010)
  • Author(s): K. Enpuku, S. Chosokabe, H. Watanabe, M. Matsuo and T. Yoshida.
  • Organizer: Applied Superconductivity Conf.(ASC2010)
  • Place of Presentation: Washington DC(USA)
  • Year and Date: 2010-08-06
• [Presentation] AC susceptibility measurement of magnetic markers for liquid phase detection of biological targets (2010)
  • Author(s): K. Enpuku, Y. Tamai, T. Mitake, T. Yoshida and M. Matsuo.
  • Organizer: 8^<th> Int. Conf. Sci. Clin. Appl. of Magnetic Carrie
  • Place of Presentation: Rostock(Germany)
  • Year and Date: 2010-05-27
• [Presentation] Detection of biological targets using Brownian relaxation of magnetic markers (2009)
  • Author(s): Y. Tamai, T. Mitake, S. Chosokabe, M. Matsuo, K. Enpuku, H. Kuma, N. Hamasaki, A. Tsukamoto, T. Mizoguchi, A. Kandori, H. Kanzaki and N. Usuki.
  • Organizer: 12^<th> Int. Superconductive Electronics Conf
  • Place of Presentation: Fukuoka(JAPAN)
  • Year and Date: 2009-06-17
• [Remarks]
  • URL: http://www.sc.kyushu-u.ac.jp/～enlab/