Study on utilization technology of oxygen-tolerant denitrifying microorganisms in sewage and wastewater treatment to prevent global warming

2012 Fiscal Year Final Research Report

• Project/Area Number: 21360258
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Civil and environmental engineering
• Research Institution: Tohoku Gakuin University
• Principal Investigator: ENDO Ginro 東北学院大学, 工学部, 教授 (80194033)
• Co-Investigator(Kenkyū-buntansha): NAKAMURA Kanji 東北学院大学, 工学部, 教授 (90382655) ; MIYAUCHI Keisuke 東北学院大学, 工学部, 教授 (20324014)
• Project Period (FY): 2009 – 2012
• Keywords: 地球温暖化ガス排出削減 / 硝化脱窒 / 温室効果ガス / 亜酸化窒素(N_2O) / 耐酸素性脱窒細菌 / 分子生物学的解析

Research Abstract

In this research project, we developed an effective method to monitor the behavior of nitrous oxide reductase gene (nosZ) in nitrification and denitrification processes by using quantitative PCR (realtime PCR) method. After the development, Pseudomonas stutzeri TR2 that was known as an oxygen tolerant bacterium was introduced into nitrification and denitrification systems, and its survival and denitrification activity were investigated. New denitrifying bacteria were isolated and selected under aerobic and nitrite-existing conditions from activated sludge obtained from a wastewater treatment plant. Among the 20 bacterial isolates, some of them were exhibited high denitrification capability with no emission of nitrous oxide under existing dissolved oxygen. These isolates also show high growth capability with nitrous oxide as a sole source of electron acceptor. Therefore, these isolates originated from activated sludge were considered to be applicable to the technology for reduction of nitrous oxide emission.

Research Products

• [Journal Article] Bioaugmentation of a wastewater bioreactor system with the nitrous oxide-reducing denitrifier Pseudomonas stutzeri strain TR2 (2013)
  • Author(s): Wakako Ikeda-Ohtsubo, Morio Miyahara,Sang-Wan Kim, Takeshi Yamada, Masaki Matsuoka, Akira Watanabe, Shinya Fushinobu, Takayoshi Wakagi, Hirofumi Shoun, Keisuke Miyauchi, Ginro Endo
  • Journal Title: Journal of Bioscience and Bioengineering Volume: Vol.115, No.1 Pages: 37-42
  • DOI: DOI:doi.org/10.1016/j.jbiosc.2012.08.015
  • Peer Reviewed
• [Journal Article] Community structure and population dynamics of ammonia oxidizers in composting processes of ammonia-rich livestock waste (2013)
  • Author(s): Takeshi Yamada, Shinya Araki, Wakako Ikeda-Ohtsubo, Keiko Okamura, Akira Hiraishi, Hideyo Ueda, Yasuichi Ueda, Keisuke Miyauchi, and Ginro Endo
  • Journal Title: Systematic and Applied Microbiology
  • URL: http://dx.doi.org/10.1016/j.syapm.2013.02.001
  • Peer Reviewed
• [Journal Article] Survival of the aerobic denitrifier Pseudomonas stutzeri strain TR2 during co-cultures with activated sludge under the denitrifying conditions (2012)
  • Author(s): Morio Miyahara, Sang-Wan Kim, Shengmin Zhou, Shinya Fushinobu, Takeshi Yamada, Wakako Ikeda-Ohtsubo, Akira Watanabe, Keisuke Miyauchi, Ginro Endo, Takayoshi Wakagi, and Hirofumi Shoun
  • Journal Title: Bioscience Biotechnology and Biochemistry Volume: Vol.76,No.3 Pages: 495-500
  • DOI: DOI:DOI:10.1271/bbb.110785
  • Peer Reviewed
• [Journal Article] コンポスト製造過程において発生する亜酸化窒素の削減に関与する脱窒細菌遺伝子の多様性と消長 (2011)
  • Author(s): 進藤絵里香、大坪和香子、上田裕一、上田英代、宮内啓介、遠藤銀朗
  • Journal Title: 土木学会論文集 G(環境) Volume: Vol.67, NO.7 Pages: 441-448
  • Peer Reviewed
• [Journal Article] Aerobic denitrification by Pseudomonas stutzeriTR2 has the potential to reduce nitrous oxide emission from wastewater treatment plants (2010)
  • Author(s): Morio Miyahara, Sang-Wan Kim, Shinya Fushinobu, Koki Takaki, TakeshiYamada, Akira Watanabe, Keisuke Miyauchi, Ginro Endo, Takayoshi Wakagi,and Hirofumi Shoun
  • Journal Title: Applied and Environmental Microbiology Volume: Vol.76, No.14 Pages: 4619-4625
  • DOI: DOI:10.1128/AEM.01983-09
  • Peer Reviewed
• [Presentation] 窒素化合物を高濃度に含む有機性廃水・廃棄物のバイオ処理プロセスにおける亜酸化窒素の発生メカニズムと発生抑制に関する研究 (2012)
  • Author(s): 遠藤銀朗
  • Organizer: 第20回北海道大学衛生工学シンポジウム
  • Place of Presentation: 北海道大学学術交流会館、北海道札幌市
  • Year and Date: 2012-12-04
• [Presentation] Pilot scale bioaugmentation for removing nitrous oxide (N2O) using denitrifying bacterium Pseudomonas stutzeri strain TR2; a case study, Environmental Microbiology and Biotechnology 2012 (2012)
  • Author(s): W. Ikeda-ohtsubo, M. Miyahara, K. Sang-Wan, A. Watanabe, S. Fushinobu, T. Wakagi, H. Shoun, K. Miyauchi, G. Endo
  • Organizer: Environmental Microbiology and Biotechnology 2012
  • Place of Presentation: School of Engineering, Alma Mater Studiorum, University of Bologna, Bologna
  • Year and Date: 2012-04-11
• [Book] IFO 微生物学概論 (2010)
  • Author(s): 遠藤銀朗、他
  • Total Pages: 15
  • Publisher: 培風館