Study of chlorophyll biosynthesis

2011 Fiscal Year Final Research Report

• Project/Area Number: 21370014
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Hokkaido University
• Principal Investigator: TANAKA Ayumi 北海道大学, 低温科学研究所, 教授 (10197402)
• Co-Investigator(Kenkyū-buntansha): TANAKA Ryouichi 北海道大学, 低温科学研究所, 准教授 (20311516)
• Project Period (FY): 2009 – 2011
• Keywords: クロロフィル / 代謝 / 酵素 / 常緑 / 細胞死

Research Abstract

We identified 7-hydroxymethyl chlorophyll a reductase (HCAR). Recombinant HCAR converted 7hydroxymethyl chlorophyll a to chlorophyll a by using ferredoxin and contains FAD and an iron-sulfur center. A phylogenetic analysis elucidated the evolution of 7-hydroxymethyl chlorophyll a reductase from divinyl chlorophyllide vinyl reductase. We examined the substrate specificity of HCAR and determined the chlorophyll b degradation pathway in which chlorophyll b is converted to chlorophyll a via 7-hydroxymethyl chlorophyll a.

Research Products

• [Journal Article] Luminescence of singlet oxygen in photosystem II complexes isolated from cyanobacterium Synechocystis sp. PCC6803 containing monovinyl or divinyl chlorophyll a. (2012)
  • Author(s): Tomo, T., Kusakabe, H., Nagao, R., Ito, H., Tanaka, A., Akimoto, S., Mimuro, M. and Okazaki, S
  • Journal Title: Biochim Biophys Acta.
  • DOI: DOI:10.1016/j.bbabio.2012.02.018
• [Journal Article] Overproduction of chlorophyll b retards senescence through transcriptional re-programming in Arabidopsis (2012)
  • Author(s): Sakuraba Y, Balazadeh S, Tanaka R, Mueller-Roeber B and Tanaka A.
  • Journal Title: Plant Cell Physiol.
  • DOI: DOI:10.1093/pcp/pcs006
• [Journal Article] Alterations in photosynthetic pigments andamino acid composition of D1 protein change energy distribution in photosystem II (2012)
  • Author(s): Yokono, M., Tomo, T., Nagao, R., Ito, H., Tanaka, A. and Akimoto, S
  • Journal Title: Biochim Biophys Acta
  • DOI: DOI:10.1016/j.bbabio.2012.02.009
• [Journal Article] Evolution of a divinyl chlorophyll-based photosystem in Prochlorococcus. (2011)
  • Author(s): Ito, H. and Tanaka, A.
  • Journal Title: Proc Natl Acad Sci U S A Volume: 108(44) Pages: 18014-18019
  • DOI: DOI:10.1073/pnas.1107590108
• [Journal Article] The Oligomeric States of Photosystems and Light-harvesting Complexes in the Chlorophyll b-less Mutant. (2011)
  • Author(s): Takabayashi, A., Kurihara, K., Kuwano, M., Kasahara, Y., Tanaka, R. and Tanaka, A.
  • Journal Title: Plant Cell Physiol Volume: 52(12) Pages: 2103-2114
  • DOI: DOI:10.1093/pcp/pcr138
• [Journal Article] AMonogalactosyldiacylglycerol Synthase Found in the Green Sulfur Bacterium Chlorobaculum tepidum Reveals Important Roles for Galactolipids in Photosynthesis. (2011)
  • Author(s): Masuda, S., Harada, J., Yokono, M., Yuzawa, Y., Shimojima, M., Murofushi, K., Tanaka, H., Masuda, H., Murakawa, M., Haraguchi, T., Kondo, M., Nishimura, M., Yuasa, H., Noguchi, M., Oh-oka, H., Tanaka, A., Tamiaki, H. and Ohta, H.
  • Journal Title: Plant Cell Volume: 23(7) Pages: 2644-2658
  • DOI: DOI:10.1105/tpc.111.085357
• [Journal Article] Identification of the 7-Hydroxymethyl Chlorophyll a Reductase of the Chlorophyll Cycle in Arabidopsis. (2011)
  • Author(s): Meguro, M., Ito, H., Takabayashi, A., Tanaka, R. and Tanaka, A.
  • Journal Title: Plant Cell Volume: 23(9) Pages: 3442-3453
  • DOI: DOI:10.1105/tpc.111.089714
• [Journal Article] Chlorophyll cycle regulates the construction and destruction of the light-harvesting complexes. (2011)
  • Author(s): Tanaka, R. and Tanaka, A.
  • Journal Title: Biophys Biochim Acta Volume: 1807 Pages: 968-976
  • DOI: DOI:10.1016/j.bbabio.2011.01.002
• [Journal Article] LIL3, alight-harvesting-like protein, plays an essential role in chlorophyll and tocopherol biosynthesis. (2010)
  • Author(s): Tanaka, R., Rothbart, M., Oka, S., Takabayashi, A., Takahashi, K., Shibata, M., Myouga, F., Motohashi, R., Shinozaki, K., Grimm, B. and Tanaka, A.
  • Journal Title: Proc Natl Acad Sci U S A Volume: 107(38) Pages: 16721-16725
  • DOI: DOI:10.1073/pnas.1004699107
• [Journal Article] Identification of a gene essential for protoporphyrinogen IX oxidase activity in the cyanobacterium Synechocystis sp. PCC6803. (2010)
  • Author(s): Kato, K., Tanaka, R., Sano, S., Tanaka, A.and Hosaka, H.
  • Journal Title: Proc Natl Acad Sci U S A Volume: 107(38) Pages: 16649-16654
  • DOI: DOI:10.1073/pnas.1000771107
• [Journal Article] The cell biology of tetrapyrroles : a life and death struggle (2010)
  • Author(s): Mochizuki, M., Tanaka, R., Grimm, B., Masuda, T., Moulin, M., Smith, AG., Tanaka, A. and Terry, MJ.
  • Journal Title: Trends Plant Sci Volume: 15(9) Pages: 488-498
  • DOI: DOI:10.1016/j.tplants.2010.05.012
• [Journal Article] Regulation and Functions of the Chlorophyll Cycle in The Chloroplast : Basics and Applications (2010)
  • Author(s): Tanaka, R., Ito, H. and Tanaka, A.
  • Journal Title: B. C. Springer Pages: 55-77
  • DOI: DOI:10.1007/978-90-481-8531-3_4
• [Journal Article] Involvement of AtNAP1 in the regulation of chlorophyll degradation in Arabidopsis thaliana (2010)
  • Author(s): Nagane, T., Tanaka, A. and Tanaka, R.
  • Journal Title: Planta Volume: 231(4) Pages: 939-949
  • DOI: DOI:10.1007/s00425-010-1099-8
• [Journal Article] Determination of a chloroplast degron in the regulatory domain of chlorophyllide a oxygenase. (2009)
  • Author(s): Sakuraba, Y., Tanaka, R., Yamasato, A. and Tanaka, A.
  • Journal Title: J Biol Chem Volume: 284(52) Pages: 6689-6699
  • DOI: DOI:10.1074/jbc.M109.008144
• [Journal Article] Participation of chlorophyll b reductase in the initial step of the degradation of light-harvesting chlorophyll a/b-protein complexes in Arabidopsis. (2009)
  • Author(s): Horie, Y., Ito, H., Kusaba, M., Tanaka, R. and Tanaka, A.
  • Journal Title: J Biol Chem. Volume: 284(26) Pages: 17449-17456
  • DOI: DOI:10.1074/jbc.M109.008912
• [Journal Article] Deregulated chlorophyll b synthesis reduces the energy transfer rate between photosynthetic pigments and induces photodamage in Arabidopsis thaliana.
  • Author(s): Sakuraba, Y., Yokono, M. Akimoto, S., Tanaka, R. and Tanaka, A.
  • Journal Title: Plant Cell Physiol. Volume: 51(6) Pages: 1055-1065
  • DOI: DOI:10.1093/pcp/pcq050
• [Presentation] Chlorophyllase1 is not involved in chlorophyll breakdown during methyl-jasmonate-induced senescence in Arabidopsis thaliana (2012)
  • Author(s): Xueyun Hu, Tohru Tsuchiya, Stefan Hoertensteiner, Ayumi Tanaka, Ryouichi Tanaka
  • Organizer: 第52回日本植物生理学会年会
  • Place of Presentation: 京都産業大学(京都市)
  • Year and Date: 2012-03-18
• [Presentation] BN-PAGEにより精製された光合成反応中心の時間分解蛍光スペクトルの測定 (2012)
  • Author(s): 横野牧生,高林厚史,栗原克宜,田中歩,秋本誠志
  • Organizer: 第52回日本植物生理学会年会
  • Place of Presentation: 京都産業大学(京都市)
  • Year and Date: 2012-03-18
• [Presentation] Divinyl Chlorophyllide Reductaseの性質 (2012)
  • Author(s): 伊藤寿、田中歩
  • Organizer: 第52回日本植物生理学会年会
  • Place of Presentation: 京都産業大学(京都市)
  • Year and Date: 2012-03-18
• [Presentation] 新規手法による葉緑体タンパク質複合体の網羅的検出 (2012)
  • Author(s): 高林厚史,栗原克宜,田中亮一,田中歩
  • Organizer: 第52回日本植物生理学会年会
  • Place of Presentation: 京都産業大学(京都市)
  • Year and Date: 2012-03-18
• [Presentation] シロイヌナズナにおける新規Light-harvesting-likeタンパク質の機能解析 (2012)
  • Author(s): 加藤由佳子、高林厚史、田中歩、田中亮一
  • Organizer: 第52回日本植物生理学会年会
  • Place of Presentation: 京都産業大学(京都市)
  • Year and Date: 2012-03-16
• [Presentation] Light-harvesting chlorophyll-binding proteinと共通するモチーフを持つタンパク質、LIL3の機能解析 (2012)
  • Author(s): 高橋香織、高林厚史、田中歩、田中亮一
  • Organizer: 第52回日本植物生理学会年会
  • Place of Presentation: 京都産業大学(京都市)
  • Year and Date: 2012-03-16
• [Presentation] シロイヌナズナのクロロフィルb分解における複数経路の検証 (2012)
  • Author(s): 下田洋輔、伊藤寿、田中歩
  • Organizer: 第52回日本植物生理学会年会
  • Place of Presentation: 京都産業大学(京都市)
  • Year and Date: 2012-03-16
• [Presentation] Evolution of chlorophyll metabolism (2011)
  • Author(s): Ayumi Tanaka
  • Organizer: The Evolution of Photosynthesis and Oxygenation of the Earth.
  • Place of Presentation: TheUniversity of New South Wales, Sydney(Australia)
  • Year and Date: 20110628-29
• [Presentation] 強光適応時のシロイヌナズナにおけるクロロフィルb還元酵素の機能解析 (2011)
  • Author(s): 中島沙織,伊藤寿,高林厚史,田中亮一,田中歩
  • Organizer: 第52回日本植物生理学会
  • Place of Presentation: 東北大学(仙台市)
  • Year and Date: 20110320-0322
• [Presentation] シロイヌナズナにおける7-ヒドロキシメチルクロロフィルa還元酵素の同定 (2011)
  • Author(s): 目黒美生,伊藤寿,高林厚史,田中亮一,田中歩
  • Organizer: 第52回日本植物生理学会
  • Place of Presentation: 東北大学(仙台市)
  • Year and Date: 20110320-0322
• [Presentation] Enzymes and functions of the chlorophyll cycle.JAPANESE-FINNISH Seminar2011 (2011)
  • Author(s): Ayumi Tanaka
  • Organizer: Future prospects of photosynthetic organisms : from genomes to environment
  • Place of Presentation: 岡山大学(岡山市)
  • Year and Date: 20110301-5
• [Presentation] Enzymes and functions of the chlorophyll cycle. (2011)
  • Author(s): Ayumi Tanaka
  • Organizer: Swiss-Japan International science seminar : Adaptation of the plastids to various environmental conditions
  • Place of Presentation: Evolution, acclimation and regulation
  • Year and Date: 20110110-14
• [Presentation] 生体金属イオン:生命と分子をつなぐメッセンジャー (2011)
  • Author(s): 田中歩
  • Organizer: 第84回日本生化学会大会シンポジウム
  • Place of Presentation: 国立京都国際会館(京都府)
  • Year and Date: 2011-09-21
• [Presentation] Roles of chlorophyll metabolism in the formation and degradation of light-harvesting complexes (2010)
  • Author(s): Ayumi Tanaka
  • Organizer: 15th International Congress of Photosynthesis
  • Place of Presentation: Beijing(China)
  • Year and Date: 20100822-27
• [Presentation] 海洋性ラン藻の亜硝酸イオン輸送体の解析 (2010)
  • Author(s): 前田真一,村上明男,伊藤寿,田中歩,小俣達男
  • Organizer: 第51回日本植物生理学会年会
  • Place of Presentation: 熊本大学黒髪キャンパス(熊本市)
  • Year and Date: 2010-03-21
• [Presentation] 恒常的免疫応答変異体nsl2の老化過程に関わる機能解析 (2010)
  • Author(s): 高橋和馬,浅田裕,山本宏子,高林厚史,田中亮一,田中歩,山口淳二
  • Organizer: 第51回日本植物生理学会年会
  • Place of Presentation: 熊本大学黒髪キャンパス(熊本市)
  • Year and Date: 2010-03-21
• [Presentation] 緑色硫黄光合成細菌から新規に単離したMGDG合成酵素はシロイヌナズナMGDG合成酵素の変異を部分的に相補する (2010)
  • Author(s): 増田真二,原田二朗,横野牧生,下嶋美恵1,室伏和博,湯澤優一,村川雅人,近藤真紀,西村幹夫,大岡宏造,田中歩,民秋均,太田啓之
  • Organizer: 第51回日本植物生理学会年会
  • Place of Presentation: 熊本大学黒髪キャンパス(熊本市)
  • Year and Date: 2010-03-20
• [Presentation] クロロフィルb蓄積変異株におけるチラコイド膜タンパク質の網羅的解析 (2010)
  • Author(s): 高林厚史,栗原克宜,櫻庭康仁,桑野晶喜,笠原康裕,田中亮一,田中歩
  • Organizer: 第51回日本植物生理学会年会
  • Place of Presentation: 熊本大学黒髪キャンパス(熊本市)
  • Year and Date: 2010-03-20
• [Presentation] クロロフィル合成およびトコフェロール合成に関与する,LHCモチーフを持つタンパク質の同定 (2010)
  • Author(s): 田中亮一,Maxi Rothbart,岡征子,柴田勝,明賀丈史,本橋令子,篠崎一雄,Bernhard Grimm,田中歩
  • Organizer: 第51回日本植物生理学会年会
  • Place of Presentation: 熊本大学黒髪キャンパス(熊本市)
  • Year and Date: 2010-03-18
• [Presentation] 植物免疫抑制因子NSL2の相互作用タンパク質の探索と解析 (2010)
  • Author(s): 浅田裕,高橋和馬,田中莉夏子,勝又邦明,山本宏子,上中弘典,長根智洋,田中亮一,田中歩,山口淳二
  • Organizer: 第51回日本植物生理学会年会
  • Place of Presentation: 熊本大学黒髪キャンパス(熊本市)
  • Year and Date: 2010-03-18
• [Presentation] シロイヌナズナの種子形成におけるクロロフィルb還元酵素の機能解析 (2010)
  • Author(s): 中島沙織,伊藤寿,田中亮一,田中歩
  • Organizer: 第51回日本植物生理学会年会
  • Place of Presentation: 熊本大学黒髪キャンパス(熊本市)
  • Year and Date: 2010-03-18
• [Presentation] クロロフィル分解に関与するフラビンタンパク質 (2010)
  • Author(s): 目黒美生,伊藤寿,高林厚史,田中亮一,田中歩
  • Organizer: 第51回日本植物生理学会年会
  • Place of Presentation: 熊本大学黒髪キャンパス(熊本市)
  • Year and Date: 2010-03-18
• [Presentation] D1タンパク質の改変によるジビニルクロロフィルへの適合性の獲得 (2010)
  • Author(s): 伊藤寿,田中亮一,田中歩
  • Organizer: 第51回日本植物生理学会年会
  • Place of Presentation: 熊本大学黒髪キャンパス(熊本市)
  • Year and Date: 2010-03-18
• [Presentation] ジビニルクロロフィルに置換したSynechocystis sp.PCC 6803 光化学系 IIの解析 (2010)
  • Author(s): 日下部勇人,岡崎茂俊,長尾遼,伊藤寿,田中歩,三室守,鞆達也
  • Organizer: 第51回日本植物生理学会年会
  • Place of Presentation: 熊本大学黒髪キャンパス(熊本市)
  • Year and Date: 2010-03-18
• [Presentation] Tetrapyrrole metabolism of photosynthetic organisms : Degrative pathways & their regulation. (2009)
  • Author(s): Ayumi Tanaka
  • Organizer: International Conference on Tetrapyrrole Photoreceptors in Photosynthetic Organisms (ICTPPO 2009)
  • Place of Presentation: Asilomar Conference Center, Pacific Grove, CA (USA)
  • Year and Date: 20090726-31
• [Presentation] Delayed leaf senescence by enhanced chlorophyll b biosynthesis. ILTS International Symposium (2009)
  • Author(s): Yasuhito Sakuraba, Ryouichi Tanaka, and Ayumi Tanaka
  • Organizer: Frontier of Low Temperature Science
  • Place of Presentation: Institute of Low Temperature Science, Hokkaido University (Sapporo)
  • Year and Date: 2009-09-09
• [Presentation] Photosystem development of marine cyanobacterium Prochlorococcus. ILTS International Symposium (2009)
  • Author(s): Hisashi Ito and Ayumi Tanaka
  • Organizer: Frontier of Low Temperature Science
  • Place of Presentation: Institute of Low Temperature Science, Hokkaido University (Sapporo)
  • Year and Date: 2009-09-09
• [Presentation] 地球環境と光合成の進化、北海道大学低温科学研究所研究集会 (2009)
  • Author(s): 田中歩
  • Organizer: 大気圏と生物圏の相互作用:大気化学を中心として
  • Place of Presentation: 北海道大学低温科学研究所(札幌市)
  • Year and Date: 2009-07-09
• [Remarks]
  • URL: http://www.lowtem.hokudai.ac.jp/plantadapt/