Mechanisms regulating developmental fate and sex differences of the germline in Drosophiala embryos

2011 Fiscal Year Final Research Report

• Project/Area Number: 21370027
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Morphology/Structure
• Research Institution: National Institutes of Natural Sciences Okazaki Research Facilities
• Principal Investigator: KOBAYASHI Satoru 大学共同利用機関法人自然科学研究機構(岡崎共通研究施設), 岡崎統合バイオサイエンスセンター, 教授 (90225508)
• Project Period (FY): 2009 – 2011
• Keywords: 生殖細胞 / 性決定 / 運命決定

Research Abstract

In this study, we have been trying to clarify the mechanisms regulating developmental fate and sex differences of the germ cells in Drosophila. What has remained a fascinating puzzle is how the germ cell decides its own sex. Here, we show that a single gene(Sxl) can initiate female sexual development in the primordial germ cells(PGCs). Our findings pave the way for future work that will help to establish the extent to which sex detemination mechanism is conserved in germ cell.

Research Products

• [Journal Article] Drosophila Sex lethal gene initiates female development in germline progenitors (2011)
  • Author(s): K. Hashiyama, Y. Hayashi and S. Kobayashi
  • Journal Title: Science Volume: 333 Pages: 885-888
  • Peer Reviewed
• [Journal Article] Innexin2 gap junctions in somatic support cells are required for cyst formation and for egg chamber formation in Drosophila (2011)
  • Author(s): M. Mukai, K. Kato, S. Hira, K. Nakamura, H. Kita and S. Kobayashi
  • Journal Title: Mech. Dev. Volume: 128 Pages: 510-523
  • Peer Reviewed
• [Journal Article] Notch and Egfr signaling act antagonistically to regulate germline stem cell niche formation in Drosophila male embryonic gonads (2010)
  • Author(s): Y. Kitadate and S. Kobayashi
  • Journal Title: Proc. Natl. Acad. Sci., USA Volume: 107 Pages: 14241-14246
  • Peer Reviewed
• [Journal Article] Small Peptides Switch the Transcriptional Activity of Shavenbaby During Drosophila Embryogenesis (2010)
  • Author(s): T. Kondo, S. Plaza, J. Zanet, E. Benrabah, P. Valenti, Y. Hashimoto, S. Kobayashi, F. Payre, Y. Kageyama
  • Journal Title: Science Volume: 39 Pages: 336-339
  • Peer Reviewed
• [Journal Article] Non-molting glossy/shroud encodes a short-chain dehydrogenase/reductase that functions in the "Black Box" of the ecdysteroid biosynthesis pathway (2010)
  • Author(s): R. Niwa, K. Ito, T. Namiki, Y. Shimada-Niwa, M. Kiuchi, S. Kawaoka, T. Kayukawa, Y. Banno, Y. Fujimoto, S. Shigenobu, S. Kobayashi, T. Shimada, S. Katsuma, and T. Shinoda
  • Journal Title: Development Volume: 137 Pages: 1991-1999
  • Peer Reviewed
• [Journal Article] Drosophila glypicans regulate the germline stem cell niche (2009)
  • Author(s): Y. Hayashi, S. Kobayashi and H. Nakato
  • Journal Title: J. Cell Biol Volume: 187 Pages: 473-480
  • Peer Reviewed
• [Journal Article] Expression of the apoptosis inducer gene head involution defective in primordial germ cells of the Drosophila embryo requires eiger, p53 and loki function (2009)
  • Author(s): T. Maezawa, K. Arita, S. Shigenobu and S. Kobayashi
  • Journal Title: Develop, Growth and Differ Volume: 51 Pages: 453-461
  • Peer Reviewed
• [Journal Article] Expression of genes involved in sumoylation in the Drosophila germline (2009)
  • Author(s): K. Hashiyama and S. Kobayashi
  • Journal Title: Gene Expression Patterns Volume: 9 Pages: 50-53
  • Peer Reviewed
• [Presentation] 「ショウジョウバエ胚における生殖細胞系列の性決定機構」シンポジウム「脊椎動物の性決定及び性分化機構ー生殖幹細胞及び生殖細胞の最先端研究」 (2011)
  • Author(s): 小林悟
  • Organizer: 第82回日本動物学会大会
  • Place of Presentation: 大雪クリスタルホール、北海道
  • Year and Date: 2011-09-23
• [Presentation] Notch and Egfr signaling act antagonistically to regulate germline stem cell niche formation in Drosophila embryos (2010)
  • Author(s): Y. Kitadate and S. Kobayashi
  • Organizer: 43rd Annual Meeting for the Japanese Society of Developmental Biology
  • Place of Presentation: 京都国際会議場京都府
  • Year and Date: 2010-06-21
• [Presentation] 「ショウジョウバエにおける配偶子幹細胞の形成を制御するメカニズム」「配偶子幹細胞制御に関する研究の新展開」シンポジウムオーガナイザー (2009)
  • Author(s): 小林悟
  • Organizer: 第80回日本動物学会大会
  • Place of Presentation: 静岡県コンベンションセンターグランシップ、静岡県
  • Year and Date: 2009-09-17
• [Book] ショウジョウバエの卵子幹細胞、「卵子学」 (2011)
  • Author(s): 浅岡美穂、小林悟
  • Total Pages: 13-24
  • Publisher: 京都大学学術出版会
• [Remarks] Hashiyama, K., Hayashi, Y. and Kobayashi, S.(2011) Drosophila Sex lethal gene initiates female development in germline progenitors. Science 333, 885-888.の発表論文に関して、以下のように新聞等で報道された。7.8(2011年月・日)朝日新聞37面、7.8中日新聞1面、7.8毎日新聞24面、7.8日本経済新聞34面、7.8日刊工業新聞21面、7.8日経産業新