2011 Fiscal Year Final Research Report
Elucidation of the transport mechanism of a secondary active transporter, Mhp1.
| Project/Area Number |
21370043
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Structural biochemistry
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
TAKUYA Kobayashi 京都大学, 医学研究科, 講師 (20311730)
SHIROISHI Mitsunori 九州大学, 薬学研究院, 助教 (00380527)
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| Project Period (FY) |
2009 – 2011
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| Keywords | X線結晶解析 / 輸送体 / ヒダントイン / トランスポーター / 交互アクセスモデル / 膜タンパク質 |
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| Research Abstract |
Transporters transport the substrates across the membrane by the alternating access mechanism in which the proteins have three functionally distinct conformational states(outward-facing, occluded, and inward-facing). Mhp1 is a secondary active transporter belonging to the LeuT superfamily. We succeeded to determine the Mhp1 structures in these three states of the alternating access mechanism. By the comparison of these structures, the conformational changes between these states are found to be achieved by the rigid body movement of the four helices relative to the rest of the protein. The molecular basis of the alternating access mechanism should be common among t he LeuT superfamily.
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[Journal Article] The Na+-hydantoin membrane transport protein2012
Author(s)
Jackson SM, Ivanova E, Simmons K, Patching SG, Weyand S, Shimamura T, Brueckner F, Iwata S, Sharples DJ, Baldwin SA, Sansom MPS, Beckstein O, Cameron AD, Henderson PJF
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Journal Title
Encyclopedia of Biophysics Springer
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[Journal Article] Cloning, expression and purification of the anion exchanger 1 homologue from the basidiomycete Phanerochaete chrysosporium2011
Author(s)
Tokuda N, Igarashi K, Shimamura T(co-1st author), Yurugi-Kobayashi T, Shiroishi M, Ito K, Sugawara T, Asada H, Murata T, Nomura N, Iwata S, Kobayashi T.
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Journal Title
Protein. Expr. Purif.
Volume: 79(1)
Pages: 81-7
DOI
Peer Reviewed
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[Journal Article] Evaluation of the Pichia pastoris expression system for the production of GPCRs for structural analysis2011
Author(s)
Asada H, Uemura T, Yurugi-Kobayashi T, Shiroishi M, Shimamura T, Tsujimoto H, Ito K, Sugawara T, Nakane T, Nomura N, Murata T, Haga T, Iwata S, Kobayashi T.
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Journal Title
Microb. Cell. Fact.
Volume: 10
Pages: 24
DOI
Peer Reviewed
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[Journal Article] The Nucleobase-Cation-Symport-1 family of Membrane Transport Proteins2011
Author(s)
Weyand S, Ma P, Beckstein O, Baldwin J, Jackson S, Suzuki S, Shimamura T, Sansom M, Iwata S, Cameron A, Baldwin S, Henderosn P.
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Journal Title
John Wiley and Sons, Ltd
Pages: 004-009
DOI
Peer Reviewed
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