2011 Fiscal Year Final Research Report
Analysis of the novel family of G protein-coupled receptor
| Project/Area Number |
21370056
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Functional biochemistry
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| Research Institution | Nara Institute of Science and Technology |
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Principal Investigator |
ITOH Hiroshi 奈良先端科学技術大学院大学, バイオサイエンス研究科, 教授 (10183005)
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| Co-Investigator(Kenkyū-buntansha) |
MIZINO Norikazu 奈良先端科学技術大学院大学, バイオサイエンス研究科, 助教 (90212232)
TAGO Kenji 奈良先端科学技術大学院大学, バイオサイエンス研究科, 助教 (20306111)
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| Project Period (FY) |
2009 – 2011
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| Keywords | 細胞情報伝達機構 / 受容体 / Gタンパク質 / 細胞遊走制御 / モノクローナル抗体 |
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| Research Abstract |
Adhesion G protein-coupled receptors(GPCRs) have a long N-terminal extracellular domain and a seven-transmembrane domain. Most of adhesion GPCRs are orphan receptors, and the activation and signal transduction mechanisms remain to be clarified. GPR56 and Latrophilin1 belong to adhesion GPCRs. We succeed in preparing the monoclonal antibody against human GPR56, which inhibits the glioma cell migration, and have constructed several mutants. Analysis using mutants showed that an extracellular domain of Latrophilin1 has an ability to inhibit the activation of Latrophilin1 transmembarane domain as well as the GPR56 transmembrane activation.
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