Molecular mechanism of the multi-transportmachinery

2012 Fiscal Year Final Research Report

• Project/Area Number: 21370057
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Functional biochemistry
• Research Institution: Okayama University
• Principal Investigator: OMOTE Hiroshi 岡山大学, 大学院・医歯薬学総合研究科, 准教授 (10273707)
• Project Period (FY): 2009 – 2012
• Keywords: 生体膜 / トランスポーター / タンパク質 / 生化学 / 神経 / 輸送

Research Abstract

The vesicular glutamate transporter (VGLUT) is a member of the SLC17 family and transports glutamate into synaptic vesicles using a membrane potential as a driving force. VGLUT plays essential roles in glutamatergic signal transmission. The SLC17 family members are unique transporter which two distinct transport activity ,membrane potential driven anion transport and Na^+ gradient driven inorganic phosphate transport. In this study, we investigated functional mechanism and roles of SLC17 transporters.

Research Products

• [Journal Article] Vesicular Neurotransmitter Transporters: An approach for studying transporters with purified proteins (2012)
  • Author(s): Omote, H., Moriyama, Y
  • Journal Title: Physiology Volume: 28 Pages: 39-50
  • DOI: doi:10.1152/physiol.00033.2012.
• [Journal Article] A Na^+ -phosphate cotransporter homologue (SLC17A4 protein) is an intestinal organic anion exporter. (2012)
  • Author(s): Togawa N, Miyaji T, Izawa S, Omote, H.Moriyama Y
  • Journal Title: Am J Physiol Cell Physiol Volume: 302 Pages: C1652-C1660
  • DOI: doi:10.1152/ajpcell.00015.2012.
• [Journal Article] Divalent cation transport by vesicular nucleotide transporter (2011)
  • Author(s): Miyaji, T., Sawada, K., Omote, H.Moriyama Y
  • Journal Title: J. Biol. Chem Volume: 286 Pages: 42881-42887
  • DOI: doi:10.1074/jbc.M111.277269.
• [Journal Article] Functional characterization of vesicular excitatory amino acid transport by human sialin. (2011)
  • Author(s): Miyaji, T., Omote, H.Moriyama Y
  • Journal Title: J.Neurochem Volume: 119 Pages: 1-5
  • DOI: doi:10.1111/j.1471-4159 11.07388.x.
• [Journal Article] Vesicular neurotransmitter transporter: bioenergetics and regulation of glutamate transport (2011)
  • Author(s): Omote, H.Miyaji, T., Juge, N. and Moriyama, Y
  • Journal Title: Biochemistry Volume: 50 Pages: 5558-5565
  • DOI: doi:10.1021/bi200567k.
• [Journal Article] Characterization of he human MATE2 proton-coupled polyspecific organic cation exporter (2011)
  • Author(s): Komatsu, T., Hiasa, M., Miyaji, T.,Kanamoto, T., Matsumoto, T., Otsuka, M., Moriyama, Y. and Omote, H
  • Journal Title: In.t J. Biochtem. Cell Biol. Volume: 43 Pages: 913-918
  • DOI: doi:10.1016/j.biocel.2011.03.005.
• [Journal Article] Metabolic control of vesicular glutamate transport and release (2010)
  • Author(s): Juge, N., Gray, J.A.,Omote, H.Miyaji, N doi: ne Iharada, M., Miyaji, T., Fujimoto, T., Hiasa, T., Inoue, T., Hara, C., Uneyama, H., Edwards, R.H., Nicoll, R.A. and Moriyama, Y.
  • Journal Title: Neuron Volume: 68 Pages: 99-112
  • DOI: doi:10.1016/j. uron.2010.09.002.
• [Journal Article] Type 1 sodium dependent phosphate transporter (SLC17A1 protein) is a Cl^- -dependent urate exporter (2010)
  • Author(s): Iharada, M., Miyaji, T., Fujimoto, T., Hiasa,M., Anzai, N., Omote, H., and Moriyama, Y
  • Journal Title: J. Biol. Chem Volume: 285 Pages: 26107-26113
  • DOI: doi:10.1074/jbc.M110.122721
• [Journal Article] Tricks of the trade used to accelerate high-resolution structure determination of membrane proteins (2010)
  • Author(s): Sonoda, Y., Cameron, A., Newstead,S., Omote, H., Moriyama, Y., Kasahara, M., Iwata, S., Drew, D.
  • Journal Title: FEBS Volume: 584 Pages: 2539-2547
  • DOI: doi:10.101 slet.2010.04.015
• [Journal Article] Vesicular inhibitory amino acid transporter is a Cl^-/γ-aminobutyrate co-transporter. (2009)
  • Author(s): Juge, N.,Muroyama, A., Hiasa, M., Omote, H.and Moriyama, Y.
  • Journal Title: J. Biol. Chem Volume: 284 Pages: 35073-35078
  • DOI: doi:10.1074 .M109.062414.
• [Presentation] NPTホモログは新規尿酸排出トランスポーターである (2012)
  • Author(s): 表 弘志
  • Organizer: 日本生体エネルギー研究会 第38回討論会
  • Place of Presentation: 岡山
  • Year and Date: 20121222-24
• [Presentation] NPTホモログは新規尿酸排出トランスポーターである (2012)
  • Author(s): 表 弘志
  • Organizer: 生体膜と薬物の相互作用シンポジウム
  • Place of Presentation: 京都
  • Year and Date: 20121115-16
• [Presentation] 小胞型グルタミン酸トランスポーターの活性制御 (2011)
  • Author(s): 表 弘志
  • Organizer: 日本生体エネルギー研究会 第36回討論会
  • Place of Presentation: 京都
  • Year and Date: 20111220-22
• [Presentation] 小胞型神経伝達物質トランスポーターの機能制御 (2011)
  • Author(s): 表 弘志
  • Organizer: 生体膜と薬物の相互作用シンポジウム
  • Place of Presentation: 岡山
  • Year and Date: 20111124-25
• [Presentation] 小胞型ヌクレオチドトランスポーターによる二価カチオン輸送 (2010)
  • Author(s): 表 弘志
  • Organizer: 日本生体エネルギー研究会 第36回討論会
  • Place of Presentation: 大阪
  • Year and Date: 20101119-20
• [Presentation] 小胞型抑制性アミノ酸トランスポーター(VIAAT)による (2009)
  • Author(s): 表 弘志
  • Organizer: Cl-輸送日本生体エネルギー研究会 第35回討論会
  • Place of Presentation: 旭川医科大学
  • Year and Date: 20091218-20
• [Presentation] 小胞型神経伝達物質トランスポーターとシアル酸輸送 (2009)
  • Author(s): 表 弘志
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸
  • Year and Date: 20091021-24
• [Remarks]
  • URL: http://www.pharm.okayama-u.ac.jp/lab/seika/home/index.html
• [Patent(Industrial Property Rights)] 哺乳動物における新規SLC17型トランスポータータンパク質およびその利用 (2013)
  • Inventor(s): 森山 芳則、表 弘志、澤田 啓介
  • Industrial Property Rights Holder: 岡山大学
  • Industrial Property Number: 特許第5219146号
  • Acquisition Date: 2013-03-15
• [Patent(Industrial Property Rights)] Novel SLC-17-type transporter protein in mammal and use thereof (2012)
  • Inventor(s): Moriyama, Y., Omote, H., Sawada, K
  • Industrial Property Rights Holder: 岡山大学
  • Industrial Property Number: 特許 (EU)、EP 2159281B1
  • Acquisition Date: 2012-09-26
  • Overseas
• [Patent(Industrial Property Rights)] 哺乳動物における新規トランスポータータンパク質およびその利用 (2011)
  • Inventor(s): 森山 芳則、表 弘志、大塚 正人、松本 拓也、日浅 未来
  • Industrial Property Rights Holder: 岡山大学
  • Industrial Property Number: 特許第4752068号
  • Acquisition Date: 2011-06-03
• [Patent(Industrial Property Rights)] Methods for screening modulators of SLC17-type anion transport activity (2011)
  • Inventor(s): Moriyama, Y., Omote, H., Sawada, K
  • Industrial Property Rights Holder: 岡山大学
  • Industrial Property Number: 特許 (米国)、US7,951,595B2
  • Acquisition Date: 2011-05-31
  • Overseas
• [Patent(Industrial Property Rights)] Novel Transporter protein in mammal and utilization of the same (2010)
  • Inventor(s): Moriyama, Y., Omot ., Otsuka, M.,Matsumoto, T., Hiasa, M
  • Industrial Property Rights Holder: 岡山大学
  • Industrial Property Number: 特許 (米国)、US7,892,728B2
  • Acquisition Date: 2010-11-04
  • Overseas