2013 Fiscal Year Final Research Report
Change of oligosaccharides in cell adhesion, and its roles
| Project/Area Number |
21370059
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Functional biochemistry
|
|---|
| Research Institution | Tohoku Pharmaceutical University |
|---|
Principal Investigator |
GU Jianguo 東北薬科大学, 薬学部, 教授 (40260369)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
FUKUDA Tomohiko 東北薬科大学, 薬学部, 准教授 (40433510)
ISAJI Tomoya 東北薬科大学, 薬学部, 助教 (80433514)
|
|---|
| Project Period (FY) |
2009-04-01 – 2014-03-31
|
| Keywords | N-glycosylation / cell adhesion / integrin / E-cadherin |
|---|
| Research Abstract |
The functional units of cell adhesion are typically multiprotein complexes made up of three general classes of proteins; the adhesion receptors, the cell-extracellular matrix (ECM) proteins, and the cytoplasmic proteins. The cell adhesion receptors are usually transmembrane glycoproteins (for example E-cadherin and integrin) that mediate binding at the extracellular surface and determine the specificity of cell-cell and cell-ECM recognition. The most of these adhesive proteins are glycosylated. In the present study, we clearly demonstrated that N-glycosylation is important for integrin-mediated cell migration and intracellular signaling, a mutual regulation between the N-glycan expression and E-cadherin-mediated cell adhesion, and the underlying molecular mechanisms.
|
-
[Journal Article] An oncogenic protein Golgi phosphoprotein 3 up-regulates cell migration via sialylation2014
Author(s)
Isaji, T., Im, S., Gu, W., Wang, Y., Hang, H., Lu, J., Fukuda, T., Hashii, N., Takakura, D., Kawasaki, N., Miyoshi, H. and Gu, J.
-
Journal Title
J Biol. Chem
Volume: (in press)
-
[Journal Article] α1, 6-Fucosylation regulates neurite formation via the activin/phospho-Smad2 pathway in PC12 cells : the implicated dual effects of Fut8 for TGF-β/activin-mediated signaling2013
Author(s)
Gu, W., Fukuda, T., Isaji, T., Hashimoto, H., Wang., Y and Gu, J.
-
Journal Title
FASEB J
Volume: 27
Pages: 3947-3958
-
[Journal Article] Core fucosylation of mu heavy chains regulates the assembly of precursor B cell receptors and its intracellular signaling2012
Author(s)
Li, W., Liu, Q., Pang, Y., Jin, J., Wang, H., Cao, H., Li, Z., Wang, X., Ma, B., Chi, Y., Wang, R., Kondo, A., Gu, J. and Taniguchi, N.
-
Journal Title
J Biol Chem
Volume: 287
Pages: 2500-2508
-
[Journal Article] Roles of N-acetylglucosaminyltransferase III in epithelial-to-mesenchymal transition induced by TGF-β1 in epithelial cell lines2012
Author(s)
Xu, Q., Isaji, T., Lu, Y., Gu, W., Kondo, M., Fukuda, T., Du, Y. and Gu, J.
-
Journal Title
J Biol Chem
Volume: 287
Pages: 16563-74
-
[Journal Article] Wnt/β-catenin Signaling Down-regulates N-Acetylglucosaminyltransferase III Expression : The implication of two mutually exclusive pathways for regulation2011
Author(s)
Xu, Q., Akama, R., Isaji, T., Lu, Y., Hashimoto, H., Kariya, Y., Fukuda, T., Du, Y., and Gu, J.
-
Journal Title
J. Biol. Chem
Volume: 286
Pages: 4310-8
-
[Journal Article] α1, 6-Fucosyltransferase-deficient mice exhibit multiple behavioral abnormalities associated with a schizophrenia-like phenotype : importance of the balance between the dopamine and serotonin systems2011
Author(s)
Fukuda, T., Hashimoto, H., Okayasu, N., Kameyama, A., Onogi, H., Nakagawasai, O, Nakazawa, T., Kurosawa, T., Hao, Y., Isaji, T., Tadano, T., Narimatsu, H., Taniguchi, N. and Gu, J.
-
Journal Title
J. Biol. Chem
Volume: 286
Pages: 18434-43
-
-
-
-
[Journal Article] An N-glycosylation site on the beta-propeller domain of the integrin alpha5 subunit plays key roles in both its function and site-specific modification by beta1, 4-N-acetylglucosaminyltransferase III2009
Author(s)
Sato, Y., Isaji, T., Tajiri, M., Yoshida-Yamamoto, S., Yoshinaka, T., Somehara, T., Fukuda, T., Wada, Y. and Gu, J.
-
Journal Title
J. Biol. Chem
Volume: 284
Pages: 11873-81
-
-
-
-
-
-
-
-
-
-
