2012 Fiscal Year Final Research Report
Elucidation of the molecular mechanism of photosynthetic water oxidation by means of infrared spectroscopy
| Project/Area Number |
21370063
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biophysics
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| Research Institution | Nagoya University (2010-2012)
University of Tsukuba (2009) |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KATO Chihiro 神奈川県産業技術センター, 化学技術部, 専門研究員 (50233804)
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| Project Period (FY) |
2009 – 2012
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| Keywords | 光合成 / 水分解 / 酸素発生 / 振動分光学 / 赤外分光 |
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| Research Abstract |
We have investigated the molecular mechanism of photosynthetic water oxidation using light-induced Fourier transform infrared difference spectroscopy and revealed the number of protons released upon individual intermediate transitions, the efficiencies of transitions, the structures and the roles of amino-acids coupled to the Mn cluster, the effect of an inhibitor on water oxidation reactions, and the regulation mechanism by extrinsic proteins. We also studied proton-coupled electron transfer in the water oxidation reaction using time-resolved infrared spectroscopy and obtained experimental evidence that proton is released before the electron transfer in the S3→S0transition, in which molecular oxygen is evolved.
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Research Products
(65 results)
