2011 Fiscal Year Final Research Report
Single-molecule analysis of functions of biomolecular machines that synthesize or assist folding of proteins.
| Project/Area Number |
21370065
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biophysics
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| Research Institution | The University of Tokyo |
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Principal Investigator |
FUNATSU Takashi 東京大学, 大学院・薬学系研究科, 教授 (00190124)
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| Project Period (FY) |
2009 – 2011
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| Keywords | 1分子計測 / ナノバイオ / 分子機械 |
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| Research Abstract |
Rapture force of single ribosome and mRNA was measured by optical tweezers. We found that IF2 helped to lock the 70S ribosome over the start codon during initiation, thus maintaining reading frame. Next, we studied chaperonin reaction cycle. It is widely believed that an asymmetric GroEL-GroES complex(termed a bullet-shaped complex) is the only form of the GroEL-GroES complex, and a symmetric GroEL-(GroES)_2 complex(termed a football-shaped complex) is not formed during the reaction cycle. However, we found that football-shaped complex was formed in the presence of sufficient amount of denatured proteins.
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