Elucidation of mechanism for conformational change and protein folding of the group 2 chaperonin

2011 Fiscal Year Final Research Report

• Project/Area Number: 21370067
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biophysics
• Research Institution: Tokyo University of Agriculture and Technology
• Principal Investigator: YOHDA Masafumi 東京農工大学, 大学院・工学研究院, 教授 (50250105)
• Project Period (FY): 2009 – 2011
• Keywords: シャペロン / シャペロニン / フォールディング / 構造変化 / 古細菌 / ストップトフロー

Research Abstract

To elucidate detailed conformational change and protein folding mechanism of the group 2 chaperonin(CPN), we have performed kinetic studies of conformational change by stopped-flow fluorometry, stopped-flow small angle X-ray scattering and Diffracted X-ray Tracking. The results have shown that the conformational change is biphasic and the latter includes the rotational motion of the ring. In addition, we constructed and characterize asymmetric ring complex that consists of a wild-type ring and a mutant ring by using circular permutated connected mutants. The results gave an insight on the inter ring communication of CPN.

Research Products

• [Journal Article] Dimer structure and conformational variability in the N-terminal region of an archaeal small heat shock protein (2011)
  • Author(s): Takeda, K., Hayashi, T., Abe, T., Hirano, Y., Hanazono, Y., Yohda, M., Miki, K.
  • Journal Title: J. Struct. Biol Volume: 174 Pages: 92-99
  • Peer Reviewed
• [Journal Article] StHsp14. 0, a small heat shock protein of Sulfolobus tokodaii strain 7, protects denatured proteins from aggregation in the partially dissociated conformation (2011)
  • Author(s): Abe, T., Oka, T., Nakagome, A., Tsukada, Y., Yasunaga, T., Yohda, M.
  • Journal Title: J. Biochem Volume: 150 Pages: 403-409
  • Peer Reviewed
• [Journal Article] Thermodynamic Characterization of the Interaction between Prefoldin and Group II Chaperonin (2010)
  • Author(s): Sahlan, M., Zako, T., Tai, P. T., Ohtaki, A., Noguchi, K., Maeda, M., Miyatake, H., Dohmae, N., Yohda, M.
  • Journal Title: J. Mol. Biol Volume: 399 Pages: 628-636
  • Peer Reviewed
• [Journal Article] Analysis of the interaction mode between hyperthermophilic archaeal group II chaperonin and prefoldin using a platform of chaperonin oligomers of various subunit arrangements (2010)
  • Author(s): Sahlan, M., Kanzaki, T., Zako, T., Maeda, M., Yohda, M.
  • Journal Title: Biochim. Biophys. Acta Volume: 1804 Pages: 1810-1816
  • Peer Reviewed
• [Journal Article] Adaptation of a hyperthermophilic group II chaperonin to relatively moderate temperatures (2010)
  • Author(s): Kanzaki, T., Ushioku, S., Nakagawa, A., Oka, T., Takahashi, K., Nakamura, T., Kuwajima, K., Yamagishi, A., Yohda, M.
  • Journal Title: Protein Eng. Des. Se Volume: 23 Pages: 393-402
  • Peer Reviewed
• [Journal Article] Characterization of a sHsp of Schizosaccharomyces pombe, SpHsp 15. 8, and the implication of its functional mechanism by comparison with another sHsp (2009)
  • Author(s): Sugino, C., Hirose, M., Tohda, H., Yoshinari, Y., Abe, T., Giga-Hama, Y., Iizuka, R., Shimizu, M., Kidokoro, S., Ishii, N., Yohda, M.
  • Journal Title: Proteins Volume: 74 Pages: 6-17
  • Peer Reviewed
• [Journal Article] Structural studies on the oligomeric transition of a small heat shock protein
  • Author(s): Hanazono, Y., Takeda, K., Yohda, M., Miki, K.
  • Journal Title: Mol. Biol. in press Volume: 14
• [Presentation] ATP dependent conformational change and protein folding mechanism of group II chaperonin (2011)
  • Author(s): M. Yohda, A. Nakagawa, K. Moriya, T. Oka, H. Sekiguchi, Y. C. Sasaki, K. Makabe, K.
  • Organizer: The 11th KIAS Conference on Protein Structure and Function, KIAS(KOREA INSTITUTE FOR ADVANCED STUDY) Seoul
  • Place of Presentation: South Korea
  • Year and Date: 2011-10-08
• [Presentation] Conformational change and protein folding mechanism of group II chaperonin proposed from stopped-flow experiments and diffracted X-ray tracking (2011)
  • Author(s): M. Yohda, A. Nakagawa, H. Sekiguchi, K. Moriya, T. Oka, K. Makabe, K. Kuwajima,. Y. C.
  • Organizer: The 3rd Pacific Protein Association Conference in conjunction with the 3rd Symposium of the Chinese Protein Society
  • Place of Presentation: Shanghai University
  • Year and Date: 2011-05-06
• [Presentation] Structure, function and application of hyperthirmophilic molecular chaperonins (2010)
  • Author(s): M. Yohda, A. Nakagawa, T. Kanzaki, H. Sekiguchi, Y. C.
  • Organizer: 2010 International Chemical Congress of Pacific Basin Societies(Pacifichem 2010), Hawaii Convention Center
  • Place of Presentation: Honolulu, USA
  • Year and Date: 2010-12-19
• [Presentation] Mechanism of protein folding by prefoldin and group II chaperonin system (2010)
  • Author(s): M. Yohda
  • Organizer: 11th China-Japan-Korea Joint Symposium on Enzyme Engineering
  • Place of Presentation: Chengdu, China
  • Year and Date: 2010-11-05
• [Presentation] Conformational change mechanism of group II chaperonin (2009)
  • Author(s): M. Yohda, T. Kanzaki, R. Masuda, A. Namagawa, K.
  • Organizer: 21th IUBMB & 12th FAOBMB International Congress of Biochemistry and Molecular Biology
  • Place of Presentation: Shanghai International Conventional Center
  • Year and Date: 2009-08-04
• [Presentation] Mechanism in Conformational Change of Group II Chaperonin EXPERIMENTAL BIOLOGY 2009(ASBMB) (2009)
  • Author(s): M. Yohda、T. Kanzaki、R. Masuda、S. Muhamad、T.
  • Organizer: Ernest N. Morial Convention Center
  • Place of Presentation: New Orleans, USA
  • Year and Date: 2009-04-21
• [Remarks]
  • URL: http://www.tuat.ac.jp/~yohda/index.html