Molecular Dissection of the Ribosomal Protein Complex that Plays a as Drive Part of Translation apparatus

2011 Fiscal Year Final Research Report

• Project/Area Number: 21370078
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Molecular biology
• Research Institution: Niigata University
• Principal Investigator: UCHIUMI Toshio 新潟大学, 自然科学系, 教授 (50143764)
• Co-Investigator(Kenkyū-buntansha): ITO Kosuke 新潟大学, 自然科学系, 助教 (20502397) ; YAO Minn 北海道大学, 大学院・先端生命科学研究院, 准教授 (40311518)
• Project Period (FY): 2009 – 2011
• Keywords: リボソーム / リボソームタンパク質 / 翻訳 / タンパク質複合体 / 結晶構造

Research Abstract

Ribosomes in all organisms have a unique flexible structure composed of ribosomal protein complex, termed the stalk, which plays a crucial role in ribosome function. In this research subject, we have investigated the structure and function of the ribosomal stalk in a hyperthermophilic archaeon by biochemical and crystallographic analyses. The results showed that the archaeal stalk is composed of aP0 and aP1, which form the heptameric complex aP0(aP1)_2(aP1)_2(aP1)_2, and that the C-terminal sequence, which is shared by aP1 and aP0, directly binds to translation factors. We concluded that the 7 copies of C-terminal tails of aP1/aP0 in the complex participate in efficient recruitment of translation factors onto the ribosome.

Research Products

• [Journal Article] Archaealribosomal stalk protein interacts with translation factors in anucleotide-independent manner via itsconserved C terminus (2012)
  • Author(s): Nomura, N., Honda, T., Baba, K., Naganuma, T., Tanzawa, T., Arisaka, F., Noda, M., Uchiyama, S., Tanaka, I., Yao, M., and Uchiumi T.
  • Journal Title: Proc. Natl. Acad. Sci. Volume: 109 Pages: 3748-3753
  • Peer Reviewed
• [Journal Article] Analysis of Chimeric Ribosomal Stalk Complexes from Eukaryotic and Bacterial Sources : Structural Features Responsible for Specificity of Translation Factors (2012)
  • Author(s): Mochizuki, M., Kitamyo, M., Miyoshi, T., Ito, K., and Uchiumi T.
  • Journal Title: Genes to Cells Volume: 17 Pages: 273-284
  • Peer Reviewed
• [Journal Article] Structural basis for thebinding of IRES RNAs to the head ofthe ribosomal 40S subunit (2011)
  • Author(s): Muhs, M., Yamamoto, H., Ismer, J., Takaku, H., Nashimoto, M., Uchiumi, T., Nakashima, N., Mielke, T., Hildebrand, P. W., Nierhaus, K. H., andSpahn, C. M.
  • Journal Title: NucleicAcids Res Volume: 39 Pages: 5264-5275
  • Peer Reviewed
• [Journal Article] An omnipotent role ofarchaeal EF1αin translationalelongation and termination, and qualitycontrol of protein synthesis (2010)
  • Author(s): Saito, K., Kobayashi, K., Wada, M., Kikuno, I., Takusagawa, A, Mochizuki, M., Uchiumi, T., Ishitani, R., Nureki, O., and Ito, K.
  • Journal Title: Proc. Natl. Acad. Sci. Volume: 107 Pages: 19242-19247
  • Peer Reviewed
• [Journal Article] Structural basis fortranslation factor recruitment to theeukaryotic/archaeal ribosomes (2010)
  • Author(s): Naganuma, T., Nomura, N., Yao, M., Mochizuki, M., Uchiumi, T., and Tanaka, I.
  • Journal Title: J. Biol. Chem Volume: 285 Pages: 4747-4756
  • Peer Reviewed
• [Journal Article] Structural basis of novel interactions between the small-GTPase and GDI-like domains in prokaryotic Feo Biron transporter (2009)
  • Author(s): Hattori, M., Jin, Y., Nishimasu, H., Tanaka, Y., Mochizuki, M., Uchiumi, T., Ishitani, R., Ito, K., and Nureki, O.
  • Journal Title: Structure Volume: 17 Pages: 1345-1355
  • Peer Reviewed
• [Journal Article] Engineering and characterization of theribosomal L10/L12 stalk complex : astructural element responsible for highturnover of the EF-G-dependent GTPase (2009)
  • Author(s): Miyoshi, T., Nomura, T., and Uchiumi, T.
  • Journal Title: J. Biol Chem Volume: 284 Pages: 85-92
  • Peer Reviewed
• [Presentation] リボソームには複数の"腕"がある (2012)
  • Author(s): 内海利男、野村直子、本田貴嘉、馬場健太朗、長沼孝雄、田中勲、姚閔
  • Organizer: 第1回RIBOSOME MEETING
  • Place of Presentation: 広島大学生物生産学部<招待>
  • Year and Date: 2012-03-15
• [Presentation] Introduction of a Lepidoptera-specific sequence with in the GTPase-associated center of 28S rRNA into Escherichia coli 23S rRNA : Effects of the mutations (2011)
  • Author(s): Ryo Maruyama, Kazuyuki Yusa, MasahiroMochizuki, Kosuke Ito, Takaomi Nomura, Toshio Uchiumi
  • Organizer: The 16_<th> Annual Meeting of theRNA Society
  • Place of Presentation: Kyoto Japan
  • Year and Date: 20110614-18
• [Presentation] 動物リボソームstalkヘテロ二量体の分子集合モードの解析 (2011)
  • Author(s): 馬場健太朗、姚閔、田中勲、内海利男
  • Organizer: 第11回日本蛋白質科学会
  • Place of Presentation: ホテル阪急エキスポパーク・大阪
  • Year and Date: 20110607-09
• [Presentation] 真核型リボソームのストークアンカータンパク質P0を構成する3ドメインの機能解析 (2010)
  • Author(s): 望月正弘、北名真澄、伊東孝祐、内海利男
  • Organizer: 第33回日本分子生物学会・第83回日本生化学会合同大会
  • Place of Presentation: 神戸ポートアイランド・神戸
  • Year and Date: 20101207-10
• [Presentation] リボソームの翻訳因子作用中心に位置する動的タンパク質複合体 (2010)
  • Author(s): 内海利男
  • Organizer: 第10回日本蛋白質科学会(ノーベル賞特別シンポジウム)
  • Place of Presentation: 札幌コンベンションセンター・札幌<招待>
  • Year and Date: 2010-06-18
• [Presentation] The stalk protein complex at the ribosomal GTPase-associated center (2010)
  • Author(s): Uchiumi, T.
  • Organizer: Current Issues of Ribosomal Structur andFunction
  • Place of Presentation: Berlin Germany<招待>
  • Year and Date: 2010-04-09
• [Presentation] 真核生物・古細菌リボソームタンパク質P0に存在する特徴的なドメインの役割 (2009)
  • Author(s): 望月正弘、長沼孝雄、内海利男
  • Organizer: 第32回日本分子生物学会
  • Place of Presentation: パシフィコ横浜・横浜
  • Year and Date: 20091209-12
• [Presentation] リボソームGTPaseセンターを構成するストークと翻訳因子の相互作用解析 (2009)
  • Author(s): 野村直子、長沼孝雄、姚閔、内海利男、田中勲
  • Organizer: 第32回日本分子生物学会
  • Place of Presentation: パシフィコ横浜・横浜
  • Year and Date: 20091209-12
• [Presentation] リボソーム16S RNA・helix 18のG505-C507、G524-C526間塩基対合の生化学的検証:EF-G依存GTPase活性への関与 (2009)
  • Author(s): 渡辺裕樹、三好智博、内海利男
  • Organizer: 第11回日本RNA学会
  • Place of Presentation: 朱鷺メッセ・新潟
  • Year and Date: 20090727-29
• [Book] ハーパー・生化学 (2011)
  • Author(s): 飯塚哲太郎、伊藤健治、内海利男、大井浩明他、岡本研、小澤敬也、上代淑人他, 19名
  • Total Pages: 812
  • Publisher: 丸善(株)
• [Remarks] ・報道関連情報(1)新潟日報(朝刊) 2012年3月8日に掲載(2)新潟大学ホームページに掲載
  • URL: http://www.niigata-u.ac.jp/top/pickup/240229.html
• [Remarks] ホームページ情報(1)研究室
  • URL: http://www.sc.niigata-u.ac.jp/biologyindex/uchiumi-ito/index.html
• [Remarks] (2)所属学科(新潟大学理学部生物学科)
  • URL: http://www.sc.niigata-u.ac.jp/biologyindex/biologyindex.html
• [Patent(Industrial Property Rights)] 大腸菌を用いたタンパク質の合成方法 (2011)
  • Inventor(s): 内海利男、朝妻悟、水口伊玖磨、山本紘、三ッ井敏明
  • Industrial Property Rights Holder: 国立大学法人新潟大学
  • Industrial Property Number: 特許、4729710
  • Acquisition Date: 2011-04-28