2011 Fiscal Year Final Research Report
Regulation of genome integrity through a DNA replication coupled feedback control
| Project/Area Number |
21370081
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Molecular biology
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| Research Institution | University of Hyogo |
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Principal Investigator |
NISHITANI Hideo 兵庫県立大学, 大学院・生命理学研究科, 教授 (40253455)
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| Co-Investigator(Kenkyū-buntansha) |
SHIOMI Yasushi 兵庫県立大学, 大学院・生命理学研究科, 助教 (80380567)
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| Project Period (FY) |
2009 – 2011
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| Keywords | DNA / 複製 / 細胞周期 / タンパク質分解 |
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| Research Abstract |
To ensure that DNA replication is limited to only once during a cell cycle, initiator protein Cdt1 is degraded once DNA replication is initiated. We examined how PCNA dependent CRL4-Cdt2 operates for the degradation of Cdt1.We report that CRL4-Cdt2 recognizes specific residues located at the N-terminus of Cdt1 and that PCNA loaders are differentially required during S phase and repair. Cdt1 degradation is strictly regulated to maintain genome integrity.
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