2011 Fiscal Year Final Research Report
Role of kinetochore stretching in controlling the spindle-assembly checkpoint
| Project/Area Number |
21370082
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Molecular biology
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| Research Institution | 公益財団法人がん研究会 (2011)
Japanese Foundation For Cancer Research (2009-2010) |
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Principal Investigator |
HIROTA Toru 公益財団法人がん研究会, がん研究所実験病理部, 部長 (50421368)
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| Co-Investigator(Renkei-kenkyūsha) |
高垣 謙太郎 公益財団法人がん研究会, がん研究所・実験病理部, 博士研究員 (70419951)
内田 和彦 公益財団法人がん研究会, がん研究所・実験病理部, 博士研究員 (40380555)
進藤 軌久 公益財団法人がん研究会, がん研究所・実験病理部, 博士研究員 (00512253)
熊田 和貴 公益財団法人がん研究会, がん研究所・実験病理部, 博士研究員 (10370149)
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| Project Period (FY) |
2009 – 2011
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| Keywords | 細胞分裂 / 染色体 / 動原体 / 微小管 / M期チェックポイント / 中期後期移行 |
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| Research Abstract |
Faithful segregation of chromosomes relies on the spindle-assembly checkpoint(SAC), which delays anaphase onset until all kinetochores are attached to microtubules(MT). The SAC monitors MT attachment at kinetochores, but whether it also senses the presence of 'tension' has long been a matter of debate. We found that 'tension' applied across sister kinetochores, which stretches centromeres, is dispensable, but stretching of kinetochores is required for silencing the SAC(Uchida et al., 2009). It is therefore crucial to investigate if 'tension' underlies kinetochore stretching. We addressed this question experimentally by engineering the conditions that microtubules are attached to kinetochors but free from the pulling force. Under these conditions, we found that kinetochores underwent stretching upon MT attachment. These results suggest that intact kinetochore-pulling force is not required for kinetochore stretching, thus challenging the notion that SAC directly monitors the presence of tension.
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[Journal Article] The initial phase of chromosome condensation requires Cdk1-mediated phosphorylation of the CAP-D3 subunit of condensin II2011
Author(s)
Abe, S., Nagasaka, K., Hirayama, Y., Kozuka-Hata, H., Oyama, M., Aoyagi, Y., Obuse, C., and Hirota, T
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Journal Title
Genes Dev
Volume: 25
Pages: 863-874
Peer Reviewed
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[Journal Article] CAMP(C13orf8, ZNF828) is a novel regulator of kinetochore-microtubule attachment2011
Author(s)
Ito, G., Kanno, S., Uchida, K. S. K, Chiba, S., Sugino, S., Watanabe, K., Mizuno, K., Yasui, A., Hirota, T., and Tanaka, K
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Journal Title
EMBO J
Volume: 30
Pages: 130-134
Peer Reviewed
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[Journal Article] Differential control of Eg5 dependent centrosome separation by Plk1 and Cdk12011
Author(s)
Smith, E., Hegarat, N., Vesely, C., Roseboom, I., Streicher, H., Straatman, K., Flynn, H., Skehel, M., Hirota, T., Kuriyama, R., Hochegger, H
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Journal Title
EMBO J
Volume: 30
Pages: 2233-2245
Peer Reviewed
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[Journal Article] Human POGZ modulates HP1 dissociation from mitotic chromosome arms through Aurora B activation2010
Author(s)
Nozawa, R., Nagao, K., Masuda, H., Iwasaki, O., Hirota, T., Nozaki, N., Kimura, H., and Obuse, C.
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Journal Title
Nat. Cell Biol
Volume: 12
Pages: 719-727
Peer Reviewed
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[Journal Article] Requirement of ATM for Rapid p53 Phosphorylation at Ser46 without Ser/Thr-Gln Sequences2010
Author(s)
Kodama, M., Otsubo, C., Hirota, T., Yokota, J., Enari, M., Taya, Y
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Journal Title
Mol. Cell. Biol
Volume: 30
Pages: 1620-1633
Peer Reviewed
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