Assembly mechanisms of the centriole precursor

2011 Fiscal Year Final Research Report

• Project/Area Number: 21370088
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Cell biology
• Research Institution: The University of Tokyo
• Principal Investigator: HIRONO Masafumi 東京大学, 大学院・理学系研究科, 准教授 (10212177)
• Project Period (FY): 2009 – 2011
• Keywords: 細胞骨格・運動 / 微小管

Research Abstract

The centriole's nine-fold symmetry is largely determined by its two precursor structures, the amorphous ring and cartwheel. However, assembly mechanisms of these structures are totally unknown. In this study, we identified a Chlamydomonas protein CRC70 as a strong candidate for an amorphous ring component, and showed that this protein induces accumulation of centriolar component when overexpressed in Chlamydomonas and mouse cells. Furthermore, by functional analysis of SAS-6 based on its X-ray crystal structure, we showed that the central part of the cartwheel is composed of radially arranged nine SAS-6 dimers.

Research Products

• [Journal Article] Scaffolding function of theChlamydomonas procentriole protein CRC70, a member of the conserved Cep70 family (2011)
  • Author(s): Shiratsuchi,G.,Kamiya,R.,and Hirono,M.
  • Journal Title: J. Cell Sci Volume: 124 Pages: 2964-2975
  • DOI: doi:10.1242/jcs.084715
  • Peer Reviewed
• [Journal Article] Structures of SAS-6 suggest its organization in centrioles (2011)
  • Author(s): van Breugel,M.,Hirono,M.,Andreeva,A.,Yanagisawa,H. A.,Yamaguchi,S.,Nakazawa,Y.,Morgner,N.,Petrovich,M.,Ebong,I.O.,Robinson,C.V.,Johnson,C.M.,Veprintsev,D.,Zuber,B.
  • Journal Title: Science Volume: 331 Pages: 1196-1199
  • DOI: doi:10.1126/science.1199325
  • Peer Reviewed
• [Journal Article] Discrete PIH proteins function in the cytoplasmic preassembly of differentsubsets of axonemal dyneins. (2010)
  • Author(s): Yamamoto,R.,Hirono,M.,and Kamiya,R.
  • Journal Title: J. Cell Biol Volume: 190 Pages: 65-71
  • DOI: doi:10.1083/jcb.201002081
  • Peer Reviewed
• [Presentation] Two novel coiled-coil axonemal proteins, Mia1 and Mia2, are required for phototaxis and normal flagellar motility in Chlamydomonas reinhardtii (2010)
  • Author(s): Yamamoto,R.,Yanagaisawa,H.,Yagi,T.,Hirono,M.,Kamiya,R.
  • Organizer: ASCB Annual Meeting
  • Place of Presentation: Philadelphia, Pennsylvania, USA
  • Year and Date: 20101211-15
• [Presentation] The Chlamydomonas flagellar I1 dynein assembles a 20S complex in the wild-type and ida3 cytoplasm, but the I1 complex fails to enter the flagellum in the ida3 mutant (2010)
  • Author(s): Viswanadha,R.,Wirschell,M.,Fox,L.,Kramer,T.,Kamiya,R.,Hirono,M.,Sale,W.S.
  • Organizer: ASCB Annual Meeting
  • Place of Presentation: Philadelphia, Pennsylvania, USA
  • Year and Date: 20101211-15
• [Presentation] A temperature- sensitive mutant in retrograde intraflagellar transport (IFT) reveals that retrograde IFT carries a distinct set of cargo proteins and is required for the maintenance of flagellar function but not flagellar length. (2010)
  • Author(s): Engel,B.D.,Wakabayashi, K.,Engel,K.B.,Geimer,S.,Hirono,M.,Kamiya,R.,and Marshall,W.F.
  • Organizer: ASCB Annual Meeting
  • Place of Presentation: Philadelphia, Pennsylvania, USA
  • Year and Date: 20101211-15
• [Presentation] TubulinPolyglutamylation Regulates FlagellarMotility by Affecting Functions ofInner-Arm Dyneins (2010)
  • Author(s): Kubo,T.,Yanagisawa,H.,Yagi,T.,Hirono,M.,Kamiya, R.
  • Organizer: 日本生物物理学会第48回年会
  • Place of Presentation: 仙台
  • Year and Date: 20100920-22
• [Presentation] A novel Chlamydomonasinner-arm-dynein-deficient mutant, ida10, harbors a mutation in a conserved protein with a PIH domain (2010)
  • Author(s): Yamamoto,R.,Hirono,M.,Kamiya,R.
  • Organizer: 14th International Conference on the Cell and Molecular Biology of Chlamydomonas
  • Place of Presentation: Wheaton College, Norton, MA, USA
  • Year and Date: 20100706-10
• [Presentation] Possible involvement of dynein regulatory complex in the regulation of inner arm dynein function by tubulin polyglutamylation (2010)
  • Author(s): Kubo,T.,Yanagisawa,H.,Yagi,T.,Hirono,M.,Kamiya,R.
  • Organizer: FASEB Summer Research ConferencesBiology of Cilia and Flagella
  • Place of Presentation: Saxtons River, Vermont, USA
  • Year and Date: 20100705-10
• [Presentation] Biochemical characterization of the cartwheel using Chlamydomonascentrioles isolated by a new method (2010)
  • Author(s): Kosugi,W.,Kamiya,R.and Hirono M.
  • Organizer: 第62回日本細胞生物学会
  • Place of Presentation: 大阪
  • Year and Date: 20100519-21
• [Presentation] Ccp201,a Chlamydomonas protein related to a mammalian centrosomal protein Cep70, functions in an early phase of centriole assembly (2010)
  • Author(s): Shiratsuchi,G.,Kamiya,R.,Hirono,M.
  • Organizer: 第62回日本細胞生物学会
  • Place of Presentation: 大阪
  • Year and Date: 20100519-21
• [Presentation] Tubulin Polyglutamylation Regulates Flagellar Motility through Modulation of Axonemal Inner-Arm Dynein Activity (2010)
  • Author(s): Kubo,T.,Yanagisawa,H.,Yagi,T.,Hirono, M.,Kamiya,R.
  • Organizer: 第62回日本細胞生物学会
  • Place of Presentation: 大阪
  • Year and Date: 20100519-21
• [Presentation] 中心子複製の謎クラミト〓モナスか〓もたらしたフ〓レークスルー (2010)
  • Author(s): 廣野雅文
  • Organizer: 日本農芸化学会2010年度大会
  • Place of Presentation: 東京
  • Year and Date: 20100327-30
• [Presentation] Assembly mechanisms of the 9+2 structure of the flagellar axoneme (2009)
  • Author(s): 廣野雅文、中澤友紀、平木まどか、白土玄、神谷律
  • Organizer: 日本分子生物学会第32会年会
  • Place of Presentation: 横浜
  • Year and Date: 20091209-12
• [Presentation] Novel features in formation and function of the "9+2" axoneme revealed by a basal-body- deficient mutant with variable numbers of triplet microtubules (2009)
  • Author(s): Nakazawa,Y.,Hiraki,M.,Kamiya,R.,and Hirono,M.
  • Organizer: American Society for Cell Biology 49th Annual Meeting
  • Place of Presentation: San Diego, U.S.A
  • Year and Date: 20091205-09
• [Presentation] Importance of tubulin polyglutamylation for axonemal dynein function as revealed by a novel Chlamydomonas mutant (2009)
  • Author(s): Kubo,T.,Yanagisawa,H.,Yagi,T.,Hirono, M.and Kamiya,R.
  • Organizer: International Workshop Dynein
  • Place of Presentation: Kobe, Japan
  • Year and Date: 20091101-05
• [Presentation] Regulation of axonemal dyneins by tubulin polyglutamylation (2009)
  • Author(s): Kubo,T.,Yanagisawa,H.,Yagi,T.,Hirono, M.and Kamiya,R.
  • Organizer: International Workshop Dynein 2009
  • Place of Presentation: Kobe, Japan
  • Year and Date: 20091101-05
• [Presentation] A novel Chlamydomonasinner-arm-dynein-deficient mutant, ida10, harbors a mutation in a conserved protein with a PIH domain (2009)
  • Author(s): Yamamoto,R.,Hirono,M.and KamiyaR.
  • Organizer: International Workshop Dynein 2009
  • Place of Presentation: Kobe, Japan
  • Year and Date: 20091101-05
• [Presentation] 中心子構築の足場として機能する新規クラミト〓モナス蛋白質Ccp201 (2009)
  • Author(s): 白土玄、神谷律、廣野雅文
  • Organizer: 日本動物学会第80回大会
  • Place of Presentation: 静岡
  • Year and Date: 20090917-19
• [Presentation] Novel features in formation and function of the "9+2" axoneme revealed by a Chlamydomonas mutant that produces basal bodies with variable numbers of triplet microtubules (2009)
  • Author(s): Yuki Nakazawa, Madoka Hiraki, Ritsu Kamiya, and Masafumi Hirono
  • Organizer: 第61回日本細胞生物学会大会
  • Place of Presentation: 名古屋
  • Year and Date: 20090602-04
• [Presentation] Importance of tubulin polyglutamylation for cilia/flagella motility as revealed by a novel Chlamydomonas mutant (2009)
  • Author(s): Tomohiro Kubo, Haru-aki Yanagisawa, Toshiki Yagi, Masafumi Hirono, and Ritsu Kamiya
  • Organizer: 第6回日本細胞生物学会大会
  • Place of Presentation: 名古屋
  • Year and Date: 20090600
• [Remarks]
  • URL: http://www.biol.s.u-tokyo.ac.jp/users/seiri/chlamy/lab.html