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2011 Fiscal Year Final Research Report

p97ATPase se洋ediated membrane fusion in the ER and Golgi

Research Project

  • PDF
Project/Area Number 21370091
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Cell biology
Research InstitutionKyushu University

Principal Investigator

KONDO Hisao  九州大学, 医学研究院, 教授研 (20205561)

Co-Investigator(Kenkyū-buntansha) TOTSUKAWA Go  九州大学, 大学院・医学研究院, 助教 (90399684)
Project Period (FY) 2009 – 2011
Keywordsゴルジ体・小胞体 / 膜融合
Research Abstract

Two distinct p97 membrane fusion pathways are required for Golgi biogenesis : the p97/p47 and p97/p37 pathwVCIPlssIP135 is necessary for both pathways, while its deubiquitinating activity is required only for the p97/p47 pathway. We have now identified a nVCIPlssIP135傭inding protein, WAC. WAC localizes to the Golgi as well as the nucleus. In Golgi membranes, WAC is involved in a complex contaiVCIPlssIP135 and p97. WAC directly bindVCIPlssIP135 and increases its deubiquitinating activity. siRNA experiments revealed that WAC is required for Golgi biogenesis. In an in vitro Golgi reformation assay, WAC was necessary only for p97/p47洋ediated Gre assemblyembly, but not for p97/p37洋ediated reassembly.

  • Research Products

    (12 results)

All 2012 2011 2010 2009 Other

All Journal Article (3 results) Presentation (7 results) Book (1 results) Remarks (1 results)

  • [Journal Article] VCIP135 deubiquitinase and its binding protein, 6VAC, in p97ATPase洋ediated membrane fusion.2011

    • Author(s)
      Totsukawa G, Kaneko Y, Uchiyama K, Toh H, Tamura K, Kondo H.
    • Journal Title

      EMBO J.

      Volume: 30(17) Pages: 3581-93

  • [Journal Article] Phosphorylation of p37 is important for Golgi disassembly at mitosis2010

    • Author(s)
      Kaneko Y, Tamura K, Totsukawa G, Kondo H.
    • Journal Title

      Biochem Biophys Res Commun

      Volume: 402(1) Pages: 37-41

  • [Journal Article] Isolation of a point洋utated p47 lacking binding affinity to p97ATPase2010

    • Author(s)
      Kaneko Y, Tamura K, Totsukawa G, Kondo H.
    • Journal Title

      FEBS Lett

      Volume: 584(18) Pages: 3837-7

  • [Presentation] A novel essential factor, p55, in p97/p47洋ediated Golgi biogenesis2012

    • Author(s)
      田村かおり
    • Organizer
      The 8th Young Invesigators Forum" Cell Migration in Biology and Medicine
    • Place of Presentation
      福岡(九州大学)
    • Year and Date
      2012-01-21
  • [Presentation] VCIP135 deubiquitinase and its binding protein, WAC, in p97 ATPase洋ediated membrane fusion2011

    • Author(s)
      十津川剛
    • Organizer
      The 10th Global COE International symposium Biochemistry and Cell Biology
    • Place of Presentation
      CeLS Auditorium, National University of Singapore
    • Year and Date
      2011-12-22
  • [Presentation] p97 ATPase洋ediated membrane fusion2011

    • Author(s)
      近藤久雄
    • Organizer
      The 8th Global COE International Symposium Kyushu University・National University of Singapore Bilateral Workshop
    • Place of Presentation
      福岡(九州大学)
    • Year and Date
      2011-08-23
  • [Presentation] Identification of a novel factor in p97/p47洋ediated Golgi membrane fusion pathway2011

    • Author(s)
      田村かおり
    • Organizer
      The 6th Young Investigators Forum
    • Place of Presentation
      Angsana Resort & Spa Bintan Site A4 Lagoi
    • Year and Date
      2011-02-12
  • [Presentation] p97ATPase洋ediated ER biogenesis2010

    • Author(s)
      近藤久雄
    • Organizer
      he 3 rd International Symposium on Proten Community
    • Place of Presentation
      ホテル日航奈良
    • Year and Date
      2010-09-13
  • [Presentation] p97 ATPase洋ediated biogenesis of the ER and Golgi2010

    • Author(s)
      金子弥生
    • Organizer
      The 5th Global COE International Symposium Cell Cycle and Differentiation
    • Place of Presentation
      Temasek Life Sciences Laboratory
    • Year and Date
      2010-02-25
  • [Presentation] p97ATPaseによる小胞体・ゴルジ体の形成維持機構2009

    • Author(s)
      近藤久雄
    • Organizer
      第61回日本細胞生物学会大会
    • Place of Presentation
      愛知(名古屋国際会議場)
    • Year and Date
      2009-06-04
  • [Book] p97ATPaseによるゴルジ体・小胞体の形成維持(細胞工学)2011

    • Author(s)
      田村かおり、森沙也子、久保田章乃、近藤久雄
  • [Remarks]

    • URL

      http://web.mac.com/hk228_01/Site/Kondo-Lab.html

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Published: 2013-07-31  

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