Comprehensive analysis of receptor-like kinase involved in MAMP recognition in Arabidopsis thaliana

2011 Fiscal Year Final Research Report

• Project/Area Number: 21380030
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Plant pathology
• Research Institution: Okayama University
• Principal Investigator: ICHINOSE Yuki 岡山大学, 大学院・自然科学研究科, 教授 (50213004)
• Co-Investigator(Kenkyū-buntansha): INAGAKI Yoshishige 岡山大学, 大学院・自然科学研究科, 准教授 (50280764)
• Project Period (FY): 2009 – 2011
• Keywords: MAMP / PAMP / エリシター / PRR / シロイヌナズナ / 受容体

Research Abstract

Bacterial non-methylated DNA was identified as one of microbe/pathogen-associated molecular patterns(MAMP/PAMP). We investigated harpin recognition system in tobacco plant as one of molecular patterns and found that an ancestor of Pseudomonas syringae pv. tabaci might have disrupted harpin gene, hrpZ by an internal deletion to evade tobacco defenses and confer the ability to cause disease in tobacco plants. Although we screened the receptor genes for bacterial DNA and/or harpin protein in Arabidopsis thaliana, the corresponding mutant lines were not identified.

Research Products

• [Journal Article] Degeneration of hrpZ gene in Pseudomonas syringae pv. tabaci to evade tobacco defence : an arms race between tobacco and its bacterial pathogen. (2011)
  • Author(s): Tsunemi, K., Taguchi, F., Marutani, M., Watanabe-Sugimoto, M., Inagaki, Y., Toyoda, T., Shiraishi, T. and Ichinose, Y.
  • Journal Title: Mol. Plant Pathol. Volume: 12(7) Pages: 709-714
  • Peer Reviewed
• [Journal Article] Bacterial DNA activates immunity in Arabidopsis thaliana (2009)
  • Author(s): Yakushiji, S., Ishiga, Y., Inagaki, Y., Toyoda, K., Shiraishi, T. and Ichinose, Y.
  • Journal Title: J. Gen. Plant Pathol. Volume: 75(3) Pages: 227-234
  • Peer Reviewed
• [Presentation] Ralstonia solanacearum RS1000株のエフェクターRip36によるナス台木植物トルバムビガーに対する過敏感反応誘導機構 (2012)
  • Author(s): 松本伊世・田口富美子・向原隆文・一瀬勇規
  • Organizer: 平成24年度日本植物病理学会大会
  • Place of Presentation: 福岡
  • Year and Date: 20120328-30
• [Presentation] 植物病原細菌Pseudomonas syringaeのべん毛・タイプIV線毛と多剤耐性 (2012)
  • Author(s): 一瀬勇規・田口富美子
  • Organizer: 第46回緑膿菌感染症研究会
  • Place of Presentation: 東京
  • Year and Date: 20120217-18
• [Presentation] Mechanism of hypersensitive reaction in Solanum torvum Sw. cv. Torubamubiga by the inoculation with Ralstonia solanacearum 2nd RS1000 (2012)
  • Author(s): Iyo Matsumoto, Fumiko Taguchi, Yoshishige Inagaki, Takafumi Mukaihara and Yuki Ichinos
  • Organizer: The 2^<nd> Korea-Japan Joint Symposium in Plant Pathology
  • Place of Presentation: 福岡
  • Year and Date: 2012-03-27
• [Presentation] Role of flagellum and pilus in motility and virulence in Pseudomonas syringae pv. Tabaci (2011)
  • Author(s): Yuki Ichinose, Chi Linh Nguyen, Fumiko Taguchi
  • Organizer: IUMS 2011(International Union of Microbiological Societies)
  • Place of Presentation: 札幌
  • Year and Date: 20110906-10
• [Presentation] 岡山大学におけるharpin, flagellin, pilin研究とこれから (2011)
  • Author(s): 一瀬勇規・田口富美子
  • Organizer: 高知大学研究拠点プロジェクト「植物健康基礎医学」
  • Place of Presentation: 高知
  • Year and Date: 20110807-08
• [Presentation] Flagellum-mediated motility and pilus-mediated sessility are important for virulence in Pseudomonas syringae pv. tabaci 6605 (2011)
  • Author(s): Ichinose Y., Asai S., Inagaki Y., Toyoda K., Shiraishi T. and Taguchi F.
  • Organizer: Asian Association of Societies for Plant Pathology(AASPP) and the Australasian Plant Pathology Society(APPS)
  • Place of Presentation: Darwin, Australia
  • Year and Date: 20110426-29
• [Presentation] ダイズ斑点病原細菌由来リポ多糖のO-抗原糖鎖の構造解析 (2011)
  • Author(s): 知久和寛, 山本雅信, 亀山眞由美, 小野裕嗣, 吉田充, 田口冨美子, 一瀬勇規, 石井忠
  • Organizer: 日本農芸化学会2011年度大会
  • Place of Presentation: 京都
  • Year and Date: 20110325-28
• [Presentation] Type IV pilin is glycosylated in Pseudomonas syringae pv. tabaci 6605 and required for surface motility and virulence (2010)
  • Author(s): Nguyen L. C., Taguchi, F., Ohnishi-Kameyama, M., Yamamoto, M., Yoshida, M., Ishii, T., Ichinose, Y.
  • Organizer: 平成22年度日本植物病理学会関西部会
  • Place of Presentation: 福井
  • Year and Date: 20100930-1001
• [Presentation] Role of flagella-mediated motility and pili-mediated sessility in the virulence of Pseudomonas syringae pv. tabaci 6605 (2010)
  • Author(s): Ichinose Y., Suzuki T., Taguchi F.
  • Organizer: 1st Asian Conference on Plant-Microbe Symbiosis and Nitrogen Fixation
  • Place of Presentation: Miyazaki
  • Year and Date: 20100920-24
• [Presentation] Role of flagellin in motility, quorum sensing and tolerance to multidrugs in Pseudomonas syringae pv. tabaci (2010)
  • Author(s): Taguchi, F., Ichinose, Y
  • Organizer: 8^<th> Pseudomonas syringae pathovars and related pathogens
  • Place of Presentation: Oxford, UK
  • Year and Date: 20100831-0903
• [Presentation] Structure and function of flagellin glycan in Pseudomonas syringae (2010)
  • Author(s): Taguchi F., Nguyen L. C., Iwaki M., Suzuki T., Yamamoto M., Ohnishi-Kameyama M., Yoshida M., Konishi T., Ishii T., Ichinose Y.
  • Organizer: The 12th International Conference on Plant Pathogenic Bacteria
  • Place of Presentation: Reunion, France
  • Year and Date: 20100607-11
• [Presentation] Pseudomonas syringae pv. tabaciにおける線毛欠損変異株の諸性質の解析 (2010)
  • Author(s): 田口富美子・鈴木智子・稲垣善茂・豊田和弘・白石友紀・一瀬勇規
  • Organizer: 平成22年度日本植物病理学会大会
  • Place of Presentation: 京都
  • Year and Date: 20100418-20
• [Presentation] 植物細菌の病原性因子とMAMP/PAMP (2010)
  • Author(s): 一瀬勇規, 田口富美子
  • Organizer: 第83回日本細菌学会総会
  • Place of Presentation: 横浜
  • Year and Date: 20100327-29
• [Presentation] Glycosylation of Bacterial Flagellins and Its Role in Motility and Virulence (2010)
  • Author(s): Ichinose Y., Taguchi F., Nguyen L. C., Naito K., Suzuki T., Inagaki Y., Toyoda K. and Shiraishi T
  • Organizer: 第10回日米科学セミナー
  • Place of Presentation: Corvallis, OR, USA
  • Year and Date: 20100124-28
• [Presentation] Amino acid sequence of bacterial Microbe-Associated Molecular Pattern flg22 is required for vilulence (2009)
  • Author(s): Kana Naito, Fumiko Taguchi, Tomoko Suzuki, Yoshishige Inagaki, Kazuhiro Toyoda, Tomonori Shiraishi and Yuki Ichinose
  • Organizer: The 2009 KSPP Fall Meeting and the 1^<st> Japan-Korea Joint Symposium
  • Place of Presentation: JeJu Korea
  • Year and Date: 20091028-31
• [Presentation] Role of Siderophore pyoverdine in the virulence of Pseudomonas syringae pv. tabaci 6605 (2009)
  • Author(s): Yuki Ichinose, Tomoko Suzuki, Yoshishige Inagaki, Kazuhiro Toyoda, Tomonori Shiraishi and Fumiko Taguchi
  • Organizer: The 2009 KSPP Fall Meeting and the 1^<st> Japan-Korea Joint Symposium
  • Place of Presentation: JeJu Korea
  • Year and Date: 20091028-31
• [Presentation] Pseudomonas syringae pv. tabaciの病原性におけるシデロフォアの役割 (2009)
  • Author(s): 田口富美子・鈴木智子・稲垣善茂・豊田和弘・白石友紀・一瀬勇規
  • Organizer: 平成21年度日本植物病理学会関西部会
  • Place of Presentation: 神戸
  • Year and Date: 20091017-18
• [Book] 第4章細菌病とファイトプラズマ病、新編植物病理学概論 (2012)
  • Author(s): 白石友紀, 秋光和也, 一瀬勇規、寺岡徹、吉川信幸
  • Total Pages: 62-85
  • Publisher: 養賢堂
• [Book] 第12章植物病理学におけるバイオサイエンス、新編植物病理学概論 (2012)
  • Author(s): 一瀬勇規, 秋光和也, 吉川信幸
  • Total Pages: 266-280
  • Publisher: 養賢堂
• [Book] Glycosylation of bacterial flagellins and its role in motility and virulence. In Genome-Enabled Analysis of Plant-Pathogen Interactions.(Wolpert, T., Shiraishi, T., Collmer A., Glazebrook, J. and Akimitsu, K. eds.) (2011)
  • Author(s): Ichinose, Y., Taguchi, F., Nguyen, L. C., Naito, K., Suzuki, T., Inagaki, Y., Toyoda, K. and Shiraishi, T
  • Total Pages: 215-224
  • Publisher: APS Press