Analysis on the recognition mechanisms of molecular pattern of plant pathogenic fungi and signaling network

2011 Fiscal Year Final Research Report

• Project/Area Number: 21380031
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Plant pathology
• Research Institution: Kyoto Prefectural University
• Principal Investigator: KUBO Yasuyuki 京都府立大学, 大学院・生命環境科学研究科, 教授 (80183797)
• Co-Investigator(Kenkyū-buntansha): MORI Masashi 石川県立大学, 生物資源工学研究所, 准教授 (00320911) ; NISHIUCHI Takumi 金沢大学, 学際科学実験センター, 准教授 (20334790) ; KOGA Hironori 石川県立大学, 生物資源環境学部, 教授 (60290079)
• Co-Investigator(Renkei-kenkyūsha): YOSHIOKA Hirofumi 名古屋大学, 生命農学研究科, 准教授 (30240245)
• Project Period (FY): 2009 – 2011
• Keywords: 植物 / 微生物 / PAMPs / バイオテクノロジ- / 炭疽病菌 / いもち病菌 / マイクロアレイ / 植物免疫植物

Research Abstract

In this research project, to elucidate plant basal resistance mechanisms against plant pathogenic fungi, modification of cell surface structure of pathogen and its involvement of recognition and signaling network leading to plant defense response was analyzed. Especially, using rice-rice blast fungus and Arabidopsis thaliana. anthracnose fungus system, signaling network downstream of pathogen recognition was extensively analyzed based on genome based approach. Manipulation of pivotal transcription factor involving disease resistance enabled us to generate disease resistant transgenic rice plants.

Research Products

• [Journal Article] HvCEBiP, a gene homologous to rice chitin receptor CEBiP, contributes to basal resistance of barley to Magnaporthe oryzae (2010)
  • Author(s): Tanaka, S., Ichikawa, A., Yamada, K., Tsuji, G., Nishiuchi, T., Mori, M., Koga, H., Nishizawa, Y., O' Connell, R., and Kubo, Y.
  • Journal Title: BMC Plant Biology Volume: 10 Pages: 288
  • Peer Reviewed
• [Journal Article] A yeast STE11 homologue CoMEKK1 is essential for pathogenesis-related morphogenesis in Colletotrichum orbiculare (2010)
  • Author(s): Sakaguchi, A., Tsuji, G., and Kubo, Y.
  • Journal Title: Molecular Plant-Microbe Interactions Volume: 23 Pages: 1563-1572
  • Peer Reviewed
• [Journal Article] Peroxisome biogenesis factor PEX13 is required for appressorium-mediated plant infection by the anthracnose fungus, Colletotrichum orbiculare (2010)
  • Author(s): Fujihara, N., Sakaguchi, A., Tanaka, S., Fujii, S., Tsuji, G., Shiraishi, T., O' Connell, R., and Kubo, Y.
  • Journal Title: Molecular Plant-Microbe Interactions Volume: 23 Pages: 436-445
  • Peer Reviewed
• [Journal Article] A Kelch repeat protein Cokel1p associates with microtubules and is involved in appressorium development in Colletotrichum orbiculare (2010)
  • Author(s): Sakaguchi, A., Miyaji, T., Tsuji, G., and Kubo, Y.
  • Journal Title: Molecular Plant-Microbe Interactions Volume: 23 Pages: 103-11
  • Peer Reviewed
• [Journal Article] Development of an efficient gene targeting system in Colletotrichum higginsianum using a non-homologous end-joining mutant and Agrobacterium tumefaciens-mediated gene transfer (2010)
  • Author(s): Ushimaru, T., Terada, H., Tsuboi, K., Kogou, Y., Sakaguchi, A., Tsuji G., and Kubo, Y.
  • Journal Title: Molecular Genetics and Genomics Volume: 284 Pages: 357-371
  • Peer Reviewed
• [Journal Article] Pathogenesis and plant basal resistance in Colletotrichum orbiculare and Magnaporthe oryzae infection. In Genome-Enabled Integration of Research in Plant Pathogen Systems (2010)
  • Author(s): Kubo, Y., and Tanaka, S, Wolpert, T., Shiraishi, T., Allen, C., Glazebrook, J. and Akimitsu, K.
  • Journal Title: APS Press Pages: 101-110
• [Journal Article] Colletotrichum : species, ecology and interactions (2010)
  • Author(s): Damm, U., Baroncelli, R, . Cai, L., Kubo, Y., O' Connell, R., Weir, B., Yoshino K., and Cannon P.
  • Journal Title: IMA Fungus Volume: 1 Pages: 161-165
  • Peer Reviewed
• [Journal Article] The Colletotrichum orbiculare ssd1 mutant enhances Nicotiana benthamiana basal resistance by activating a mitogen-activated protein kinase pathway (2009)
  • Author(s): Tanaka, S., Ishihama, N., Yoshioka, H., Huser, A., O' Connell, R., Tsuji, G., Tsuge, S., and Kubo, Y.
  • Journal Title: Plant Cell Volume: 21 Pages: 2517-2526
  • Peer Reviewed
• [Journal Article] RRS1 and RPS4 provide a dual resistance-gene system against fungal and bacterial pathogens (2009)
  • Author(s): Narusaka, M., Shirasu, K., Noutoshi, Y., Kubo, Y., Shiraishi, T., Iwabuchi M., and Narusaka, Y.
  • Journal Title: Plant Journal Volume: 59 Pages: 672-683
  • Peer Reviewed
• [Journal Article] A dual resistance gene system prevents infection by three distinct pathogens (2009)
  • Author(s): Narusaka, M., Kubo, Y., Shiraishi, T., Iwabuchi, M., and Narusaka, Y.
  • Journal Title: Signaling & Behavior Volume: 4 Pages: 954-955
  • Peer Reviewed
• [Presentation] Identification and characterization of a novel pathogenicity-associated gene CoPRF1 in the anthracnose fungus Colletotrichum orbiculare (2012)
  • Author(s): Kaoru Tanaka and Yasuyuki Kubo
  • Organizer: 2nd Korea-Japan Joint Symposium
  • Place of Presentation: Fukuoka, Japan
  • Year and Date: 20120000
• [Presentation] Characterization of CoIRA1 of Colletotrichum orbiculare, an anthracnose disease fungus of cucumber, required for infection-related morphogenesis and pathogenicity (2012)
  • Author(s): Ken Harata and Yasuyuki Kubo
  • Organizer: 2nd Korea-Japan Joint Symposium
  • Place of Presentation: Fukuoka, Japan
  • Year and Date: 20120000
• [Presentation] Colletotrichum orbiculare CoBUB2, the homolog of Saccharomyces cerevisiae BUB2, is involved in morphogenesis and pathogenesis (2012)
  • Author(s): Fumi Fukada, Ayumu Sakaguchi, and Yasuyuki Kubo
  • Organizer: 2nd Korea-Japan Joint Symposium
  • Place of Presentation: Fukuoka, Japan
  • Year and Date: 20120000
• [Presentation] Functional analysis of a novel pathogenicity-associated gene CoPRF1 in the anthracnose fungus Colletotrichum orbiculare (2011)
  • Author(s): Kaoru Tanaka and Yasuyuki Kubo
  • Organizer: 11th European conference on Fungal Generics
  • Place of Presentation: Marburg, Germany
  • Year and Date: 20110000
• [Presentation] Colletotrichum orbiculare Fam1p, a pex22 like peroxin unique in filamentous ascomycete, is required for appressorium mediated infection and associates with Woronin body (2011)
  • Author(s): Ayumu Sakaguchi, Naoki Fujihara1, Gento Tsuji, Ulla Neumann, Richard O' Connell and Yasuyuki Kubo
  • Organizer: Asian Mycological Congress
  • Place of Presentation: Seoul, Korea
  • Year and Date: 20110000
• [Presentation] Appressorium function in Colletotrichum orbiculare and prospect for genome based analysis (2011)
  • Author(s): Yasuyuki Kubo
  • Organizer: 14th Fungal Genetics and Biology Conference of the Microbiological Society of Korea
  • Place of Presentation: Chungnam, Korea
  • Year and Date: 20110000
• [Presentation] Fam1p, a peroxin unique to filamentous ascomycete fungi, is required for pathogenesis and is transferred to Woronin bodies in Colletotrichum orbiculare (2010)
  • Author(s): Ayumu Sakaguchi, Naoki Fujihara, Gento Tsuji, Ulla Neumann, Richard O' Connell and Yasuyuki Kubo
  • Organizer: 9th International Mycological Congress
  • Place of Presentation: Edinburg, United Kindom
  • Year and Date: 20100000
• [Presentation] Novel peroxin Fam1p of Colletotrichum orbiculare is essential for pathogenesis and associates with Woronin bodies (2010)
  • Author(s): Ayumu Sakaguchi, Naoki Fujihara, Gento Tsuji, Ulla Neumann, Richard O' Connell and Yasuyuki Kubo
  • Organizer: 10th European conference on Fungal Generics
  • Place of Presentation: Edinburg, United Kindom
  • Year and Date: 20100000
• [Presentation] Fungal cell surface PAMPs trigger plant basal resistance through activation of mitogen-activated protein kinase pathway (2009)
  • Author(s): Shigeyuki Tanaka, Nobuaki Ishihama, Hirofumi Yoshioka, Gento Tsuji, Seiji Tsuge and Yasuyuki Kubo
  • Organizer: 14th International Congress on Molecular Plant-Microbe Interactions Quebec
  • Place of Presentation: Canada
  • Year and Date: 20090000
• [Presentation] ウリ類炭疽病菌RAS GTPase activated proteinCoIRA1は感染器官の分化や病原性に関与する
  • Author(s): 原田賢・久保康之
  • Organizer: 平成24年度日本植物病理学会大会
• [Presentation] ウリ類炭疽病菌の出芽酵母BUB2ホモログ遺伝子CoBUB2は形態形成および病原性に関与する
  • Author(s): 深田史美・坂口歩・久保康之
  • Organizer: 平成24年度日本植物病理学会大会
• [Presentation] イネいもち病菌感染初期過程におけるミトコンドリア凝集反応の観察
  • Author(s): 望月進・齋藤憲一郎・久保康之・南栄一・西澤洋子
  • Organizer: 平成24年度日本植物病理学会大会
• [Presentation] Genomic and transcriptomic analysis of the hemibiotrophic plant pathogen Colletotrichum orbiculare
  • Author(s): Gan, P., Narusaka, Y., Takano, Y., Kubo, Y., and Shirasu, K.
  • Organizer: 平成24年度日本植物病理学会大会
• [Presentation] デュアル抵抗性蛋白質システムによる病原体認識機構の解明:RPS4とRRS1遺伝子の作物への導入
  • Author(s): 鳴坂真理・白須賢・久保康之・白石友紀・畠山勝徳・平井正良・河本晃一・江面浩・高野義孝・岩渕雅樹・鳴坂義弘
  • Organizer: 平成23年度日本植物生理学会大会
• [Presentation] NAC-like transcription factors VOZは生物学的ストレスと非生物学的ストレスとの切り換え制御に関与する
  • Author(s): 中井勇介・中平洋一・住田浩規・安居佑季子・河内孝之・高木優・光田展隆・久保康之・佐藤雅彦
  • Organizer: 日本植物学会平成23年度大会
• [Presentation] ウリ類炭疽病菌(Colletotrichum orbiculare) 104-T(MAFF240422)のゲノム解析
  • Author(s): 高野義孝・多賀正節・鳴坂義弘・白須賢・久保康之
  • Organizer: 平成23年度日本植物病理学会大会
• [Presentation] ウリ類炭疽病菌の核型と染色体の細胞学的解析
  • Author(s): 多賀正節・田中薫・久保康之
  • Organizer: 平成23年度日本植物病理学会大会
• [Presentation] デュアルR-遺伝子システムによる病原菌認識機構の解明
  • Author(s): 鳴坂真理・白須賢・久保康之・豊田和弘・白石友紀・岩渕雅樹・鳴坂義弘
  • Organizer: 平成22年度日本植物病理学会大会
• [Presentation] ウリ類炭疽病菌の侵入器官の形態形成と病原性
  • Author(s): 久保康之
  • Organizer: 2011年第11回糸状菌分子生物学コンファレンス
• [Book] Appressorium Function in Colletotrichum orbiculare and Prospect for Genome Based Analysis. In Morphogenesis and Pathogenicity in Fungi Series : Topics in Current Genetics (2011)
  • Author(s): Kubo, Y, Perez-Martin, Jose and Di Pietro, Antonio
  • Total Pages: 276
• [Remarks]
  • URL: http://ykubo.blog.eonet.jp/