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2011 Fiscal Year Final Research Report

Analysis of the mechanisms of tomato mosaic virus RNA replication complex formation

Research Project

  • PDF
Project/Area Number 21380033
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Plant pathology
Research InstitutionNational Institute of Agrobiological Sciences

Principal Investigator

ISHIKAWA Masayuki  独立行政法人農業生物資源研究所, 植物・微生物間相互作用研究ユニット, ユニット長 (70192482)

Project Period (FY) 2009 – 2011
Keywords感染・増殖 / ウイルス / 植物 / 複製
Research Abstract

A host seven-pass transmembrane protein TOM1 and a small GTP-binding protein ARL8 are required for efficient multiplication of tomato mosaic virus(ToMV) RNA. When in vitro ToMV RNA replication reactions using evacuolated plant protoplast extracts were performed using Arabidopsis thaliana membranes that lack either one of these proteins, negative-strand RNA synthesis was hardly detectable. When the replication protein of ToMV was co-expressed with TOM1 and ARL8, the proteins gained guanylyltransferase activity, but could not catalize ToMV RNA replication. Thus, interaction with TOM1-and ARL8-containing membranes confers guanylyltransferase activity to ToMV replication protein, however, it is not sufficient for the activation of its RNA polymerase activity.

  • Research Products

    (13 results)

All 2012 2011 2010 2009

All Journal Article (4 results) (of which Peer Reviewed: 4 results) Presentation (6 results) Book (3 results)

  • [Journal Article] A host small GTP-binding protein ARL8 plays crucial roles in tobamovirus RNA replication2011

    • Author(s)
      Masaki Nishikiori, Masashi Mori, Koji Dohi, Hideyasu Okamura, Etsuko Katoh, Satoshi Naito, Tetsuo Meshi and Masayuki Ishikawa
    • Journal Title

      PLoS Pathogens

      Volume: 7(12) Pages: e1002409

    • Peer Reviewed
  • [Journal Article] Arabidopsis cell-free extract, ACE, a new in vitro translation system derived from Arabidopsis callus cultures2011

    • Author(s)
      Katsunori Murota, Yuka Hagiwara-Komoda, Keisuke Komoda, Hitoshi Onouchi, Masayuki Ishikawa and Satoshi Naito
    • Journal Title

      Plant and Cell Physiology

      Volume: 52(8) Pages: 1443-1453

    • Peer Reviewed
  • [Journal Article] Gaining replicability in a nonhost by Tobacco mild green mosaic virus compromises its silencing suppression activity in a host2011

    • Author(s)
      Kazuhiro Ishibashi, Tetsuo Meshi and Masayuki Ishikawa
    • Journal Title

      J. Virol

      Volume: 85(4) Pages: 1893-1895

    • Peer Reviewed
  • [Journal Article] Interactions between Tobamovirus Replication Proteins and Cellular Factors : Their Impacts on Virus Multiplication2010

    • Author(s)
      Kazuhiro Ishibashi, Masaki Nishikiori, and Masayuki Ishikawa
    • Journal Title

      Mol. Plant-Microbe Interact

      Volume: 23(11) Pages: 1413-1419

    • Peer Reviewed
  • [Presentation] トマトモザイクウイルス複製タンパク質のジスルフィド結合を介した高分子量化2012

    • Author(s)
      錦織雅樹、飯哲夫、石川雅之
    • Organizer
      平成24年度日本植物病理学会大会
    • Place of Presentation
      福岡
    • Year and Date
      20120300
  • [Presentation] A plant small GTP-binding protein ARL8 plays a crucial role in Tobamovirus RNA replication2011

    • Author(s)
      Masaki Nishikiori, Tetsuo Meshi, Masayuki Ishikawa
    • Organizer
      International Union of Microbiological Societies 2011 Congress
    • Place of Presentation
      札幌
    • Year and Date
      20110900
  • [Presentation] トバモウイルスRNA複製における宿主因子TOM1, ARL8の機能解析2011

    • Author(s)
      錦織雅樹、森正之、土肥浩二、岡村英保、加藤悦子、内藤哲、飯哲夫、石川雅之
    • Organizer
      日本植物病理学会報
    • Year and Date
      20110000
  • [Presentation] Genomic approaches to interactions between plant viruses their hosts and their vectors2010

    • Author(s)
      Masaki Nishikiori, Tetsuo Meshi, Masayuki Ishikawa
    • Organizer
      Toward understanding the mechanisms of tobamovirus replication complex formation EMBO Workshop 2010
    • Place of Presentation
      Fenestrelle(イタリア)
    • Year and Date
      20100600
  • [Presentation] NMR緩和測定による低分子量Gタンパク質NtARL8の立体構造変化の解析2009

    • Author(s)
      岡村英保、錦織雅樹、相宏宇、石川雅之、加藤悦子
    • Organizer
      第48回NMR討論会
    • Place of Presentation
      福岡
    • Year and Date
      20091100
  • [Presentation] Host proteins for virus accumulation or resistance2009

    • Author(s)
      Masayuki Ishikawa
    • Organizer
      Their association with replication proteins of tobamoviruses the 9th International Plant Molecular Biology Congress
    • Place of Presentation
      St. Louis,米国
    • Year and Date
      20091000
  • [Book] トバモウイルスと植物との相互作用.遺伝2010

    • Author(s)
      石橋和大、石川雅之
    • Total Pages
      60-65
  • [Book] トバモウイルスの宿主域制限機構.蛋白質核酸酵素2010

    • Author(s)
      石橋和大、石川雅之
    • Total Pages
      88-93
  • [Book] 内在性遺伝子の発現抑制によるウイルス抵抗性植物の作出.農業技術2009

    • Author(s)
      石川雅之
    • Total Pages
      145-148

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Published: 2013-07-31  

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