Development of effective promoters and efficient systems for gene function analysis in insects

2012 Fiscal Year Final Research Report

• Project/Area Number: 21380041
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied entomology
• Research Institution: National Institute of Agrobiological Sciences
• Principal Investigator: SEZUTSU Hideki 独立行政法人農業生物資源研究所, 遺伝子組換えカイコ研究開発ユニット, ユニット長 (70342805)
• Co-Investigator(Kenkyū-buntansha): KOMOTO Natuo 独立行政法人農業生物資源研究所, 遺伝子組換えカイコ研究開発ユニット, 主任研究員 (30355747) ; TOMITA Shuichiro 独立行政法人農業生物資源研究所, 遺伝子組換えカイコ研究開発ユニット, 主任研究員 (30360457) ; HATAKEYAMA Masatsugu 独立行政法人農業生物資源研究所, 昆虫成長制御研究ユニット, 主任研究員 (50281142) ; NIIMI Teruyuki 名古屋大学, 大学院生命農学研究科, 助教 (00293712)
• Project Period (FY): 2009 – 2012
• Keywords: 昆虫分子生物学 / トランスジェニック昆虫

Research Abstract

Systems to efficiently analyze gene function in insects were developed. Site-specific recombination works in silkworm and the system was found to be applicable for the clonal analysis and enhancer trapping. Gene editing utilizing artificial nucleases becomes possible. A promoter of baculovirus promoted expression of transgenes in some species belonging to different orders suggesting its utility across the insects.

Research Products

• [Journal Article] Tightly controlled Tetracycline-inducible transcription system for explosive gene expression in cultured silkworm cells (2013)
  • Author(s): Tatsuke T, Lee JM, Iiyama K, Sezutsu H, Uchino K, Kusakabe T
  • Journal Title: Archives of Insect Biochemistry & Physiology Volume: 82(4) Pages: 173-182
  • DOI: DOI:10.1002/arch.21083
• [Journal Article] Efficient disruption of endogenous Bombyx gene by TAL effector nucleases (2013)
  • Author(s): Sajwan S, Takasu Y, Tamura T, Uchino K, Sezutsu H, Zurovec M
  • Journal Title: Efficient disruption of endogenous Bombyx gene by TAL effector nucleases Volume: 43(1) Pages: 17-23
  • DOI: DOI:10.1016/j.ibmb.2012.10.011
• [Journal Article] A survey of the effectiveness of non-cell autonomous RNAi throughout development in the sawfly, Athalia rosae(Hymenoptera) (2013)
  • Author(s): Yoshiyama N, Tojo K, Hatakeyama M
  • Journal Title: Journal of Insect Physiology Volume: 59(4) Pages: 400-407
  • DOI: DOI:10.1016/j.jinsphys.2013.01.009
• [Journal Article] Precocious metamorphosis in the juvenile hormone-deficient mutant of the silkworm, Bombyx mori (2012)
  • Author(s): Daimon T., Sezutsu H. 他14名
  • Journal Title: PLoS Genetics Volume: 8 (3) Pages: e1002486
  • DOI: DOI:10.1371/journal.pgen.1002486
• [Journal Article] A visible dominant marker for insect transgenesis (2012)
  • Author(s): Osanai-Futahashi M, Ohde T, Hirata J, Uchino K, Futahashi R, Tamura T, Niimi T, Sezutsu H
  • Journal Title: Nature communications Volume: 3 Pages: 1295
  • DOI: DOI:10.1038/ncomms2312
• [Journal Article] PhiC31 integrase-mediated cassette exchange in silkworm embryos (2012)
  • Author(s): Yonemura N, Tamura T, Uchino K, Kobayashi I, Tatematsu K, Iizuka T, Sezutsu H, Muthulakshmi M, Nagaraju J, Kusakabe T
  • Journal Title: Molecular Genetics and Genomics Volume: 287(9) Pages: 731-739
  • DOI: DOI:10.1007/s00438-012-0711-y
• [Journal Article] Identification of the Bombyx red egg gene reveals the involvement of a novel transporter family gene in the late steps of the insect ommochrome biosynthesis pathway (2012)
  • Author(s): Osanai-Futahashi M, Tatematsu KI, Yamamoto K, Narukawa J, Uchino K, Kayukawa T, Shinoda T, Banno Y, Tamura T, Sezutsu H.
  • Journal Title: Journal of Biological Chemistry Volume: 287(21) Pages: 17706-17714
  • DOI: DOI:10.1074/jbc.M111.321331
• [Journal Article] Development of a clonal analysis system in the silkworm, Bombyx mori, mediated by FLP and FRT (2012)
  • Author(s): Komoto N, Tomita S
  • Journal Title: Journal of Insect Biotechnology and Sericology Volume: 81 Pages: 63-67
  • DOI: DOI:10.11416/jibs.81.2_3_063
• [Journal Article] A baculovirus immediate-early gene, ie1, promoter drives efficient expression of a transgene in both Drosophila melanogaster and Bombyx mori (2012)
  • Author(s): Masumoto, M., Ohde, T., Shiomi, K., Yaginuma, T. and Niimi, T.
  • Journal Title: PLoS ONE Volume: 7 Pages: e49323
  • DOI: DOI:10.1371/journal.pone.0049323
• [Journal Article] SID-1 protein of Caenorhabditis elegans mediates uptake of dsRNA into Bombyx cells (2012)
  • Author(s): Kobayashi I, Tsukioka H, Komoto N, Uchino K, Sezutsu H, Tamura T, Kusakabe T, Tomita S
  • Journal Title: Insect Biochemistry and Molecular Biology Volume: 42 Pages: 148-154
  • DOI: DOI:10.1016/j.ibmb.2011.11.007
• [Journal Article] An Efficient Binary System for Gene Expression in the Silkworm, Bombyx mori, Using GAL4 Variants (2011)
  • Author(s): Kobayashi I., Sezutsu H. 他10名
  • Journal Title: Archives of Insect Biochemistry & Physiology Volume: 76 Pages: 195-210
  • DOI: DOI:10.1002/arch.20402
• [Journal Article] Targeted mutagenesis in the silkworm Bombyx mori using zinc finger nuclease mRNA injection (2010)
  • Author(s): Takasu, Y. 他8名
  • Journal Title: Insect Biochemistry and Molecular Biology Volume: 40 Pages: 759-765
  • DOI: DOI:10.1016/j.ibmb.2010.07.012
• [Journal Article] Extensive synteny conservation of holocentric chromosomes in Lepidoptera despite high rates of local genome rearrangement (2010)
  • Author(s): d'Alencon, E., Sezutsu, H., et al.
  • Journal Title: Proc Natl Acad Sci U S A Volume: 107 Pages: 7680-7685
  • DOI: DOI:10.1073/pnas.0910413107
• [Journal Article] Transgenic analysis of the BmBLOS2 gene that governs the translucency of the larval integument of the silkworm, Bombyx mori (2010)
  • Author(s): Fujii, T. 他7名
  • Journal Title: Insect Molecular Biology Volume: 19 Pages: 659-667
  • DOI: DOI:10.1111/j.1365-2583.2010.01020.x
• [Journal Article] Rescue of the Aojuku white-egg translucent (w-3ol) Bombyx mori mutant by transgenic expression of the wild-type Bmwh3 gene (2010)
  • Author(s): Kobayashi, I. 他6名
  • Journal Title: Journal of Insect Biotechnology and Sericology Volume: 79 Pages: 111-116
  • DOI: DOI:10.11416/jibs.79.3_111
• [Journal Article] The Silkworm-An Attractive BioResource Supplied by Japan (2010)
  • Author(s): Banno Y., Shimada T., Kajiura Z., Sezutsu H
  • Journal Title: Experimental Animals Volume: 59 Pages: 139-146
  • DOI: DOI:10.1538/expanim.59.139
• [Journal Article] Transgenic tools for members of the genus drosophila with sequenced genomes (2010)
  • Author(s): Holtzman S., Miller D., Eisman,R.C., Kuwayama H.,Niimi T., Kaufman T.C
  • Journal Title: Fly Volume: 4 Pages: 349-362
  • DOI: DOI:10.4161/fly.4.4.13304
• [Journal Article] KAIKObase: An integrated silkworm genome database and data mining tool (2009)
  • Author(s): Shimomura M. 他14名
  • Journal Title: BMC Genomics Volume: 10 Pages: 486
  • DOI: DOI:10.1186/1471-2164-10-486
• [Journal Article] The sawfly, Athalia rosae ruficornis (Hymenoptera) as a model insect for developmental and reproductive biology: what has been done and what should be done? Proc. Arthropod. Embryol (2009)
  • Author(s): Hatakeyama M., et al.
  • Journal Title: Soc. Jpn, Volume: 44 Pages: 1-12
  • URL: http://aesj.co-site.jp/paesj.html
• [Presentation] Genetically modified silkworms and silks - recent updates and future prospects (2012)
  • Author(s): Sezutsu H, Iizuka T, Uchino K, Kobayashi I, Tatematsu KI, Tsubota T, Yonemura N, Okada E, Nakajima K, Machii H, Tamura T
  • Organizer: The 7th International Conference on Wild Silkmoth and Silk
  • Place of Presentation: Mahasarakham Univ., Thailand
  • Year and Date: 20121122-24
• [Presentation] Athalia rosae (2012)
  • Author(s): Hatakeyama M
  • Organizer: an emerging genetic model system for sawflies XXIV International Congress of Entomology: S1107TH07,
  • Place of Presentation: Daegu, Korea
  • Year and Date: 2012-08-23
• [Presentation] Transgenic silkworms for analysis of gene function and production of recombinant proteins/silks (2012)
  • Author(s): Sezutsu H, Tsubota T, Osanai-Futahashi M, Suzuki T, Tatematsu KI, Kobayashi I, Iizuka T, Uchino K, Yonemura N, Takasu Y, Tamura T
  • Organizer: ICE2012 - XXIV International Congress of Entomology2012
  • Place of Presentation: Korea
  • Year and Date: 2012-08-20
• [Presentation] Pigment synthesis pathway genes and their application to visible transgenic markers (2012)
  • Author(s): Osanai-Futahashi M, Tatematsu KI, Yamamoto K, Uchino K, Futahashi R, Tamura T, Sezutsu H
  • Organizer: ICE2012 - XXIV International Congress of Entomology 2012
  • Place of Presentation: Korea
  • Year and Date: 2012-08-20
• [Presentation] Transgenic silkworm as a tool for functional analysis of insect genes and for bioreactor (2011)
  • Author(s): Tamura, T. et al.
  • Organizer: 2nd International Symposium on Silkworm Functional Genomics and Modern Silk Road
  • Place of Presentation: Chongqing, China
  • Year and Date: 20111021-24
• [Presentation] Development of transgenic silkworm Bombyx mori for functional genomics (2011)
  • Author(s): Sezutsu, H. et al.
  • Organizer: Sixth International Symposium on Molecular Insect Science
  • Place of Presentation: Sixth International Symposium on Molecular Insect Science
  • Year and Date: 20111002-05
• [Presentation] Development of transgenic silkworms for functional genomics in Bombyx mori as a lepidopteran model insect 5th Annual Arthropod Genomics Symposium (2011)
  • Author(s): Sezutsu, H. et al.
  • Organizer: 5th Annual Arthropod Genomics Symposium
  • Place of Presentation: Kansas, USA
  • Year and Date: 20110709-12
• [Presentation] Transgenic ladybird: development of gene function analysis systems for non-model insects (2011)
  • Author(s): Niimi, T
  • Organizer: The 1st International Conference on the Criket / RNAi Symposium for Medicine-Agriculture-Engineering Collaboration Project (Joint Meeting)
  • Place of Presentation: (要旨集)
  • Year and Date: 2011-03-14
• [Presentation] カブラハバチにおけるプロモーター探索の試み (2010)
  • Author(s): 畠山正統
  • Organizer: 第31回菅平動物学セミナー
  • Place of Presentation: 長野・筑波大菅平高原実験センター
  • Year and Date: 2010-12-04
• [Presentation] Development of transposon mutagenesis systems and a database for transposon insertion lines in silkworm Bombyx mori (2010)
  • Author(s): Sezutsu, H. et al.
  • Organizer: International Symposium; New Silk Road: Silkworm Genome to Sustainable Agriculture
  • Place of Presentation: Tsukuba, Japan
  • Year and Date: 2010-11-10
• [Presentation] Development of transgenic silkworm as a tool for functional analysis and for bioreactor (2010)
  • Author(s): Tamura, T. et al.
  • Organizer: International Symposium; New Silk Road: Silkworm Genome to Sustainable Agriculture
  • Place of Presentation: Tsukuba, Japan
  • Year and Date: 2010-11-10
• [Presentation] Extensive synteny conservation of holocentric chromosomes in lepidoptera despite high rates of local genome rearrangement (2010)
  • Author(s): Sezutsu, H. et al.
  • Organizer: Workshop on Recent Advances in Sericulture Research
  • Place of Presentation: Bengalure, India
  • Year and Date: 2010-05-18
• [Presentation] Transgenic silkworm as a tool for the analysis of gene function and bioreactor (2010)
  • Author(s): Tamura, T. et al.
  • Organizer: Workshop on Recent Advances in Sericulture Research
  • Place of Presentation: Bengalure, India
  • Year and Date: 2010-05-18
• [Presentation] 新規ミューテーターを用いたカイコ・エンハンサートップ系統の作出と解析, (2010)
  • Author(s): 内野恵郎他
  • Organizer: 平成22年度蚕糸・昆虫機能学術講演会.日本蚕糸学会第80回大会
  • Place of Presentation: 上田市、信州大学
  • Year and Date: 2010-04-04
• [Presentation] Construction of an enhancer trap system and Bombyx Trap DataBase for the analysis of gene function in silkworm Bombyx mori (2009)
  • Author(s): Sezutsu H., et al.
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2009-12-11
• [Presentation] 形質転換昆虫におけるBmNPVie1プロモーターの汎用性の検討 (2009)
  • Author(s): 大出高弘・増本三香・柳沼利信・新美輝幸
  • Organizer: 日本蚕糸学会中部支部第65回・東海支部大会第61回
  • Place of Presentation: 名古屋大学野依記念学術交流館・会議室
  • Year and Date: 2009-12-03
• [Presentation] Transgenic silkworm as a tool for the analysis of gene function.International Symposium on Bombyx mori (2009)
  • Author(s): Sezutsu H., et al.
  • Organizer: Functional Genomics and Modern Silk Road
  • Place of Presentation: Chongqing, China
  • Year and Date: 2009-10-23
• [Presentation] Development of transgenic silkworm Bombyx mori for functional genomics (2009)
  • Author(s): Sezutsu H., et al.
  • Organizer: The 6th Asia-Pacific Congress of Entomology
  • Place of Presentation: Beijing, China
  • Year and Date: 2009-10-20
• [Presentation] トランスジェニックカイコを用いた遺伝子機能解析 (2009)
  • Author(s): 瀬筒秀樹
  • Organizer: 2009年度 昆虫ポストゲノム研究会
  • Place of Presentation: 北海道大学 農学部総合研究棟
  • Year and Date: 2009-09-10
• [Presentation] トランスジェニックカイコおよびトランスジェニックフライにおけるBmNPVie1プロモーターのレポーターアッセイ (2009)
  • Author(s): 大出高弘・増本三香・柳沼利信・新美輝幸
  • Organizer: 2009年度 昆虫ポストゲノム研究会
  • Place of Presentation: 北海道大学 農学部総合研究棟
  • Year and Date: 2009-09-10
• [Presentation] Construction of enhancer trap system and Bombyx Trap DataBase for the analysis of gene function in silkworm Bombyx mori
  • Author(s): Sezutsu H., et al.
• [Book] 分子昆虫学 (第7章4 : 遺伝子組換え技術の害虫防除への利用) (2009)
  • Author(s): 畠山正統
  • Total Pages: 394-396
  • Publisher: 共立出版
• [Book] 分子昆虫学 (第1章6 : 遺伝子組換え技術) (2009)
  • Author(s): 瀬筒秀樹、冨田正浩
  • Total Pages: 37-39
  • Publisher: 瀬筒秀樹、冨田正浩