2012 Fiscal Year Final Research Report
Development of effective promoters and efficient systems for gene function analysis in insects
Project/Area Number |
21380041
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Applied entomology
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Research Institution | National Institute of Agrobiological Sciences |
Principal Investigator |
SEZUTSU Hideki 独立行政法人農業生物資源研究所, 遺伝子組換えカイコ研究開発ユニット, ユニット長 (70342805)
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Co-Investigator(Kenkyū-buntansha) |
KOMOTO Natuo 独立行政法人農業生物資源研究所, 遺伝子組換えカイコ研究開発ユニット, 主任研究員 (30355747)
TOMITA Shuichiro 独立行政法人農業生物資源研究所, 遺伝子組換えカイコ研究開発ユニット, 主任研究員 (30360457)
HATAKEYAMA Masatsugu 独立行政法人農業生物資源研究所, 昆虫成長制御研究ユニット, 主任研究員 (50281142)
NIIMI Teruyuki 名古屋大学, 大学院生命農学研究科, 助教 (00293712)
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Project Period (FY) |
2009 – 2012
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Keywords | 昆虫分子生物学 / トランスジェニック昆虫 |
Research Abstract |
Systems to efficiently analyze gene function in insects were developed. Site-specific recombination works in silkworm and the system was found to be applicable for the clonal analysis and enhancer trapping. Gene editing utilizing artificial nucleases becomes possible. A promoter of baculovirus promoted expression of transgenes in some species belonging to different orders suggesting its utility across the insects.
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[Journal Article] A baculovirus immediate-early gene, ie1, promoter drives efficient expression of a transgene in both Drosophila melanogaster and Bombyx mori2012
Author(s)
Masumoto, M., Ohde, T., Shiomi, K., Yaginuma, T. and Niimi, T.
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Journal Title
PLoS ONE
Volume: 7
Pages: e49323
DOI
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[Presentation] Genetically modified silkworms and silks - recent updates and future prospects2012
Author(s)
Sezutsu H, Iizuka T, Uchino K, Kobayashi I, Tatematsu KI, Tsubota T, Yonemura N, Okada E, Nakajima K, Machii H, Tamura T
Organizer
The 7th International Conference on Wild Silkmoth and Silk
Place of Presentation
Mahasarakham Univ., Thailand
Year and Date
20121122-24
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[Presentation] Transgenic silkworms for analysis of gene function and production of recombinant proteins/silks2012
Author(s)
Sezutsu H, Tsubota T, Osanai-Futahashi M, Suzuki T, Tatematsu KI, Kobayashi I, Iizuka T, Uchino K, Yonemura N, Takasu Y, Tamura T
Organizer
ICE2012 - XXIV International Congress of Entomology2012
Place of Presentation
Korea
Year and Date
2012-08-20
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