2011 Fiscal Year Final Research Report

Proteins of an entomopoxvirus : its proteomic analysis and a study for the development of technology for the use of its protein fusolin as a synergist of viral insecticides

Research Project

Project/Area Number	21380044
Research Category	Grant-in-Aid for Scientific Research (B)
Allocation Type	Single-year Grants
Section	一般
Research Field	Applied entomology
Research Institution	National Institute of Agrobiological Sciences
Principal Investigator	MITSUHASHI Wataru 独立行政法人農業生物資源研究所, 昆虫微生物機能研究ユニット, 上級研究員 (00414946)
Project Period (FY)	2009 – 2011
Keywords	昆虫ポックスウイルス / 核多角体病ウイルス / ゲノム解析 / プロテオーム解析 / 酵母外来遺伝子発現系 / 代替え宿主
Research Abstract	The genome of an alphaentomopoxvirus virus was completely sequenced for the first time. The genome of the virus that infects a coleopteran, Anomala cuprea(AcEPV) is ca. 246 kb in length, which is smaller than those of Alphaentomopoxvirus previously considered, and consists of a central coding region bounded by inverted terminal repeats(ITR) that is the longest ITRs among wild type of poxviruses reported. The genome harbors ca. 250 open reading frames. An ORF which contains a serine protease inhibitor(serpins) domain sequence, which may harbor an immunological function in mammalians not insects was detected. Fusolin gene, which expresses a viral enhancing factor, was introduced into an expression system of foreign genes in yeast for the purpose of large amount of the production of the protein for the development of a system for the enhancement of virus insecticides.