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2011 Fiscal Year Final Research Report

Molecular Mechanism of selective transport for carotenoid in the silkworm, Bombyx mori

Research Project

  • PDF
Project/Area Number 21380045
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied entomology
Research InstitutionNational Institute of Infectious Diseases

Principal Investigator

TSUCHIDA Kozo  国立感染症研究所, 放射能管理室, 室長 (40231435)

Co-Investigator(Kenkyū-buntansha) YAMAMOTO Kimiko  独立行政法人農業生物資源研究所, 昆虫ゲノム報解析ユニット, ユニット長 (40370689)
SEZUTSU Hideki  独立行政法人農業生物資源研究所, 遺伝子組換えカイコ研究センター, センター長 (70342805)
Project Period (FY) 2009 – 2011
Keywordsカロテノイド / 脂質の選択的輸送 / CD36 / SR-BI / リポホリン / カイコ
Research Abstract

The silkworm, Bombyx mori provides very clear examples of tissue-specific delivery for certain carotenoid species from the midgut lumen to the silk gland via hemolymph lipoprotein, lipophorin. Lipophorin contains lutein and beta-carotene as a complex mixture of lipids in the hydrophobic core. The yellow(C) or flesh color cocoon(F) mutants accumulate lutein or beta-carotene in the middle silk gland from lipophorin, respectively. We have reported that the C gene encodes Cameo2, a member of CD36 gene family which has been known to function as as lutein specific transporter and transmembrane non-internalizing lipophorin receptor in the middle silk gland. The nature of the F gene was defined by positional cloning and transgenic rescue experiments with the binary GAL4-UAS expression system. The F gene encodes SCRB15, a paralog of Cameo2 with 26% amino acid identity. SCRB15 may be responsible for beta-carotene specific non-internalizing lipophorin receptor. In the F mutant, SCRB15 is not expressed because the mRNA structure of SCRB15 is severely disrupted due to a 1.4 kb insertion in a coding exon. This finding that both C and F genes encode CD36 homologs raises questions how CD36-homolog proteins recoginize the difference of carotenoid species which permits to uptake the middle silk gland from lipophorin.
We believe the molecular machineries are for delivery of specific lipids from lipophorin to target tissues. Furthermore, functional coordination of transmembrane lipoprotein receptors and intracellular lipid-binding proteins has been proposed to achieve the selective cellular transport from HDL for general lipids, such as cholesteryl ester and fatty acids. Thus, our data also provide genetic evidence for this general mechanism in lipid biology.

  • Research Products

    (29 results)

All 2012 2011 2010 2009 Other

All Journal Article (14 results) (of which Peer Reviewed: 14 results) Presentation (10 results) Book (3 results) Remarks (1 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Possible overestimation of the effect of acetylation on the lysine residue employing the KQ mutant analysis2012

    • Author(s)
      Tsuchida K.(著者12, 12番)
    • Journal Title

      J. Computational Chem

      Volume: 33 Pages: 239-246

    • DOI

      DOI:10.1128/AEM.00142-12

    • Peer Reviewed
  • [Journal Article] SID-1 protein of Caenorhabditis elegans mediates uptake of dsRNA into Bombyx cell2012

    • Author(s)
      Sezutsu H.(著者8, 5番)
    • Journal Title

      Insect Biochem Mol Biol

      Volume: 42 Pages: 148-154

    • Peer Reviewed
  • [Journal Article] Diversity in copy number and structure of a silkworm morphogenetic gene as result of domestication2011

    • Author(s)
      Tsuchida K.(著者12, 12番)
    • Journal Title

      Genetics

      Volume: 187 Pages: 965-976

    • Peer Reviewed
  • [Journal Article] An Efficient Binary System for Gene Expression in the Silkworm, Bombyx mori, Using GAL4 Variants2011

    • Author(s)
      Sezutsu H.(著者12, 4番)
    • Journal Title

      Arch. Insect Biochem. Physiol

      Volume: 76 Pages: 195-210

    • Peer Reviewed
  • [Journal Article] A CD36-related transmembrane protein is coordinated with an intercellular lipid-binding protein in selective carotenoid transport for cocoon coloration2010

    • Author(s)
      Tsuchida K.(著者15, 15番)
    • Journal Title

      J. Biol. Chem

      Volume: 285 Pages: 7739-7751

    • Peer Reviewed
  • [Journal Article] Extensive synteny conservation of holocentric chromosomes in Lepidoptera despite high rates of local genome rearrangement2010

    • Author(s)
      Sezutsu H.(著者12, 2番)
    • Journal Title

      Proc Natl Acad Sci USA

      Volume: 107 Pages: 7680-7685

    • Peer Reviewed
  • [Journal Article] Apolipophorin-III expression and low density lipophorin formation during embryonic development of the silkworm2010

    • Author(s)
      Tsuchida K.(著者11, 1番)
    • Journal Title

      Bombyx mori. Comp. Biochem. Physiol

      Volume: 155 Pages: 363-370

    • Peer Reviewed
  • [Journal Article] Construction of a binary transgenic gene expression system for recombinant protein production in the middle silk gland of the silkworm Bombyx mori2010

    • Author(s)
      Sezutsu H.(著者7, 4番)
    • Journal Title

      Transgenic Res

      Volume: 19 Pages: 473-487

    • Peer Reviewed
  • [Journal Article] Purification and expression analysis of imaginal disc growth factor in the silkworm2009

    • Author(s)
      Tsuchida K.(著者9, 9番)
    • Journal Title

      Bombyx mori. J. Insect Physiol

      Volume: 55 Pages: 1065-1071

    • Peer Reviewed
  • [Journal Article] Purification and characterization of a human retinal lutein-binding protein from human retina2009

    • Author(s)
      Tsuchida K., Bernstein P.(著者7, 6番)
    • Journal Title

      Biochem

      Volume: 48 Pages: 4798-4807

    • Peer Reviewed
  • [Journal Article] KAIKObase : An integrated silkworm genome database and data mining tool2009

    • Author(s)
      Sezutsu H.(著者15, 10番)
    • Journal Title

      BMC Genomics

      Volume: 10 Pages: 486

    • Peer Reviewed
  • [Journal Article] カイコゲノム研究の現状とその利用2009

    • Author(s)
      山本公子,三田和英
    • Journal Title

      植物防疫

      Volume: 63 Pages: 1-6

    • Peer Reviewed
  • [Journal Article] カイコゲノム研究の現状と今後の展開2009

    • Author(s)
      山本公子
    • Journal Title

      蚕糸・昆虫バイオテック

      Volume: 78 Pages: 99-102

    • Peer Reviewed
  • [Journal Article] Molecular Evolution of mariner-like elements in the genus Drosophila : Theobscura species group

    • Author(s)
      Tsuchida K.(著者9, 8番)
    • Journal Title

      J. Insect Biotech. Sericol

      Volume: (In Press)

    • Peer Reviewed
  • [Presentation] カイコLipid transfer particleの分子性質,アポタンパク質mRNA発現の発育変動2012

    • Author(s)
      横山洋
    • Organizer
      日本蚕糸学会
    • Place of Presentation
      福岡
    • Year and Date
      20120300
  • [Presentation] カイコ黄繭遺伝子Cameo2の転写を制御するCameo2上流域の探索2012

    • Author(s)
      湯浅正志
    • Organizer
      日本蚕糸学会
    • Place of Presentation
      福岡
    • Year and Date
      20120300
  • [Presentation] Cameo2とCBP発現S2細胞によるルテインの取り込み2012

    • Author(s)
      永山春菜
    • Organizer
      日本蚕糸学会
    • Place of Presentation
      福岡
    • Year and Date
      20120300
  • [Presentation] クラスBスカベンジャー受容体相同遺伝子の強制発現による繭の着色2012

    • Author(s)
      作道隆
    • Organizer
      日本蚕糸学会
    • Place of Presentation
      福岡
    • Year and Date
      20120300
  • [Presentation] カイコLipid Transfer Particleは成虫期に高い発現を示す2011

    • Author(s)
      横山洋
    • Organizer
      日本蚕糸学会合同支部大会
    • Place of Presentation
      盛岡
    • Year and Date
      20111100
  • [Presentation] カイコ黄繭遺伝子の組織特異的発現を制御するシス領域の解析2011

    • Author(s)
      湯浅正志,作道隆,藤本浩文,本田尚子,普後一、土田耕三
    • Organizer
      日本蚕糸学会合同支部大会
    • Place of Presentation
      盛岡
    • Year and Date
      20111100
  • [Presentation] SR-BIファミリータンパク質が血液リポタンパク質から細胞へ移行するカロテノイドを識別する2011

    • Author(s)
      作道隆
    • Organizer
      カロテノイド研究会
    • Place of Presentation
      つくば
    • Year and Date
      20111000
  • [Presentation] Development of transgenic silkworms for functional genomics in Bombyx mori as a lepidopteran model insect2011

    • Author(s)
      Sezutsu H
    • Organizer
      Arthropod Genomics
    • Place of Presentation
      Manhattan
    • Year and Date
      20110800
  • [Presentation] Development of transgenic silkworm Bombyx mori for functional genomics2011

    • Author(s)
      Sezutsu H
    • Organizer
      6th Int. Symp. Molec. Insect Sci
    • Place of Presentation
      Amsterdam
    • Year and Date
      20110700
  • [Presentation] Molecular machinery for the tissue specific uptake of specific carotenoid in the silkworm2011

    • Author(s)
      Tsuchida K
    • Organizer
      Macular carotenoids and AMD
    • Place of Presentation
      Cambridge
    • Year and Date
      20110500
  • [Book] The Silkworm-An Attractive BioResource Supplied by Japan2011

    • Author(s)
      Sezutsu H
    • Total Pages
      139-46
  • [Book] 遺伝子組換え技術・分子昆虫学「ポストゲノムの昆虫研究」2009

    • Author(s)
      瀬筒秀樹
    • Total Pages
      36-45
    • Publisher
      共立出版
  • [Book] Transport of carotenoids by a carotenoid-binding protein in the silkworm. In : Carotenoids : Physical, Chemical, and Biological Functions and Properties2009

    • Author(s)
      Sakudoh T, Tsuchida K
    • Total Pages
      511-523
    • Publisher
      CRC Press
  • [Remarks] 化学工業新聞報道

  • [Patent(Industrial Property Rights)] キサントフィル含有赤色繭及び赤色絹糸2009

    • Inventor(s)
      土田耕三
    • Industrial Property Rights Holder
      土田耕三、富士化学工業株式会社
    • Industrial Property Number
      特願、2009-158341
    • Filing Date
      2009-07-03

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Published: 2013-07-31  

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