2013 Fiscal Year Final Research Report
Study on the control of gene expression network in Streptomyces growing in the soil environment
| Project/Area Number |
21380050
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Plant nutrition/Soil science
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| Research Institution | National Institute for Agro-Environmental Sciences |
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Principal Investigator |
FUJII Takeshi 独立行政法人農業環境技術研究所, その他部局等, 研究領域長 (00354100)
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| Co-Investigator(Kenkyū-buntansha) |
SAITO Akihiro 静岡理工科大学, 理工学部, 准教授 (50375614)
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| Project Period (FY) |
2009-04-01 – 2014-03-31
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| Keywords | 土壌生物 / 遺伝子発現 / マイクロアレイ解析 / 放線菌 / キチナーゼ |
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| Research Abstract |
To provide new insights into the cellular response to nutrients and regulation cascade of antibiotic production genes in global gene expression of Streptomyces in soil, genome-wide transcriptional analysis with microarray technology was performed using RNA extracted from the wild type and mutant strains of Streptomyces coelicolor grown in soil cultures. Microarray analyses revealed that expression of genes for secondary metabolism was induced by chitin together with primary metabolic genes in soil cultures of Streptomyces coelicolor. The data also indicated that a transcriptional factor, DasR, was involved in the regulation of gene expression for chitin catabolism, secondary metabolism and stress responses.
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Research Products
(46 results)
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[Journal Article] Chitin-induced gene expression in secondary metabolic pathways of Streptomyces coelicolor A3(2) grown in soil2013
Author(s)
Nazari, B., Kobayashi, M., Saito, A., Hassaninasab, A., Miyashita, K., Fujii, T.
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Journal Title
Appl. Environ. Microbiol
Volume: 79
Pages: 707-713
DOI
Peer Reviewed
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[Journal Article] Enzymatic and genetic characterization of the DasD protein possessing N-acetyl-β-D- glucosaminidase activity in Streptomyces coelicolor A3(2)2013
Author(s)
Saito, A., Ebise, H., Orihara, Y., Murakami, S., Sano, Y., Sugiyama, Y., Ando, A., Fujii, T., Miyashita, K.
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Journal Title
F EMS Microbiol. Lett
Volume: 340
Pages: 33-40
DOI
Peer Reviewed
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[Journal Article] Specific gene responses of Rhodococcus jostii RHA1 during growth in soil2012
Author(s)
Iino, T., Wang, Y., Miyauchi, K., Kasai, D., Masai, E., Fujii, T., Ogawa, N., Fukuda M.
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Journal Title
Appl. Environ. Microbiol
Volume: 78(19)
Pages: 6954-6962
DOI
Peer Reviewed
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[Journal Article] High expression levels of chitinase genes in Streptomyces coelicolor A3(2) grown in soil2011
Author(s)
Nazari, B., Kobayashi, M., Miyashita, K., Saito, A., Wang, Y., Fujii, T
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Journal Title
F EMS Microbiol. Ecol
Volume: 77
Pages: 623-635
Peer Reviewed
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[Journal Article] Molecular and enzymatic characterization of a subfamily I.4 lipase from an edible oil degrader Bacillus sp. HH-012011
Author(s)
Kamijo, T., Saito, A., Ema, S., You, I., Hayashi, H., Nagata, R., Nagata, Y., Ando, A.
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Journal Title
Anton. Leeuw. Int. J . G
Volume: 99
Pages: 179-187
DOI
Peer Reviewed
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[Journal Article] Streptomyces coavervatus sp. nov., isolated from the intestinal tract of Armadillidium vulgae2011
Author(s)
Shibasaki, A., Kudo, T., Yaguchi, T., Saito, A., Ando, A., Mikami, Y., Gonoi, T.
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Journal Title
Int. J . Syst. Enviol. Micr
Volume: 61(ijs.0.019091-0v161/5/1073)
Pages: 1073-1077
Peer Reviewed
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[Journal Article] Biological control of Rhizoctonia damping-off of cucumber by a transformed Pseudomonas putida strain expressing a chitinase from a marine bacterium2011
Author(s)
Ohno, M., Kataoka, S., Numata, S., Yamamoto-Tamura, K., Fujii, T., Nakajima, M., Akutsu, K., Hasebe, A.
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Journal Title
J ARQ-J pn. Agr. Res. Q
Volume: 45(1)
Pages: 91-98
URL
Peer Reviewed
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[Journal Article] Assessment of the effects of genetically modified Pseudomonas spp. expressing chitinase on the soil microbial community in the cucumber rhizosphere2011
Author(s)
Yamamoto-Tamura, K., Ohno M., Fujii T., Kataoka, S., Numata, S., Nakajima, M., Hasebe A., Akutsu K.
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Journal Title
JARQ-Jpn. Agr. Res. Q
Volume: 45(4)
Pages: 377-383
URL
Peer Reviewed
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[Presentation] Microbial gene expression analysis in soil using microarray technology2011
Author(s)
Wang, Y., Nagaoka, K., Morimoto, S., Ogawa, N., Hayatsu, M., and Fujii T.
Organizer
第27回日本微生物生態学会大会
Place of Presentation
京都大学
Year and Date
2011-10-09
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[Presentation] Chitinase production and N,N'- diacetylchitobiose uptake in Streptomyces coelicolor A3(2)2010
Author(s)
Saito, A., Fujii, T., Shinya, T., Shibuya, N., Ando, A., Miyashita, K.
Organizer
The 11th International Conference on Chitin and Chitosan Symposium
Place of Presentation
台湾, 台湾理工科大学
Year and Date
2010-09-08
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