Oligomer forming mechanism of lectin-like oxidized LDL receptor-1(LOX-1), and its functional reconstitution.

2011 Fiscal Year Final Research Report

• Project/Area Number: 21380068
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied biochemistry
• Research Institution: National Agriculture and Food Research Organization
• Principal Investigator: MACHIDA Sachiko 独立行政法人農業・食品産業技術総合研究機構, 食品総合研究所・食品バイオテクノロジー研究領域, 上席研究員 (30353981)
• Co-Investigator(Kenkyū-buntansha): ISHIKAWA Yuko 独立行政法人農業・食品産業技術総合研究機構, 食品総合研究所・食品機能研究領域, 上席研究員 (40353940) ; WATANABE Jun 独立行政法人農業・食品産業技術総合研究機構, 食品総合研究所・食品機能研究領域, 主任研究員 (10374729)
• Project Period (FY): 2009 – 2011
• Keywords: LOX-1 / オリゴマー化 / サンドイッチELISA / ビオチン化 / 競合ELISA / 酸化LDL / 酸化LDL受容体

Research Abstract

Lectin-like oxidized LDL receptor-1(LOX-1) recognizes oxidized LDL and initiates endothelial dysfunction, which is believed to be a crucial early step in atherosclerosis. Here we show how LOX-1 forms the functional unit, and succeed to reconstitute the binding ability of LOX-1.Moreover, we develop molecular device to evaluate the OxLDL formation and molecular species, which produced on OxLDL as the results of oxidative stress.

Research Products

• [Journal Article] Identification of 4-hydroxy-2-nonenal-histidine adducts that serve as ligands for human lectin-like oxidized LDL receptor-1 (2012)
  • Author(s): M. K. Kuramochi, Y. Shimozu, C. Wakita, M. O. Kameyama, T. Shibata, S. Matsunaga, Y. T. Ishikawa, J. Watanabe, M. Goto, Q. Xie, S. Komba, K. Uchida, S. Machida
  • Journal Title: The Biochemical Journal Volume: 442 Pages: 171-180
  • DOI: DOI:10.1942/BJ20111029
  • Peer Reviewed
• [Journal Article] A new method for cleavage of silicon-carbon linkers on glass plate supports with applications to solid-phase syntheses on silica resins (2010)
  • Author(s): T. Terauchi, S. Machida and S. Komba
  • Journal Title: Tetrahedron Letters Volume: 51 Pages: 1497-1499
  • DOI: DOI:10.1016/j.tetlet.2010.01.042
  • Peer Reviewed
• [Journal Article] Lipid peroxidation geberates a body order component trans-2-nononal covalently bound to protein in vivo (2010)
  • Author(s): K. Ishino, C. Wakita, T. Shibata, S. Toyokuni, S. Machida, S. Matsuda, T. matsuda, and K. Uchida
  • Journal Title: J. Biol. Chem. Volume: 285 Pages: 15302-15313
  • DOI: DOI:10.1074/jbc.M109.068023
  • Peer Reviewed
• [Journal Article] Minimum stable structure of the receptor for advanced glyction end products possesses multi ligand binding ability (2009)
  • Author(s): M. Kumano-Kuramochi, M. Ohnishi-Kameyama, Q. Xie, S. Niimi, S. Komba, and S. Machida
  • Journal Title: Biochem. Biophys. Res. Commun. Volume: 386 Pages: 130-136
  • DOI: DOI:10.1016/j.bbrc.2009.05.142
  • Peer Reviewed
• [Presentation] 酸化LDL受容体LOX-1のラフト局在におけるパルミトイル化の影響 (2011)
  • Author(s): 倉持(熊野)みゆき、謝秋宏、今場司朗、町田幸子
  • Organizer: 第84回日本生化学会
  • Place of Presentation: 京都国際会館
  • Year and Date: 2011-09-23
• [Presentation] Lectin-like oxidized LDL receptor-1(LOX-1), A critical player in the development of atherosclerosis (2011)
  • Author(s): S. Machida
  • Organizer: 吉林大学招待講演
  • Place of Presentation: 中国吉林大学
  • Year and Date: 2011-08-22
• [Presentation] HNE-histidine付加体の酸化LDL受容体LOX-1リガンドとしての機能 (2010)
  • Author(s): 倉持(熊野)みゆき、下津祐樹、脇田知佳、亀山(大西)眞由美、松永茂、謝秋宏、今場司朗、内田浩二、町田幸子
  • Organizer: 第33回日本分子生物学会年会、第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸国際会議場
  • Year and Date: 20101200
• [Presentation] 後期糖化生成物を認識する受容体(RAGE)の安定な機能単位 (2009)
  • Author(s): 倉持(熊野)みゆき、亀山(大西)眞由美、謝秋宏、新美節子、久保田富佐子、今場司朗、町田幸子
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸国際会議場
  • Year and Date: 2009-10-22
• [Patent(Industrial Property Rights)] リガンド様活性を有する分子の検出法法 (2010)
  • Inventor(s): 町田幸子、倉持みゆき、石川祐子、後藤真生、渡辺純
  • Industrial Property Rights Holder: 独立行政法人農業・食品産業技術総合研究機構
  • Industrial Property Number: 特許、特願2010-25840
  • Filing Date: 2010-11-18