Study of recognition mechanism of pathogenic bacteria in plant

2011 Fiscal Year Final Research Report

• Project/Area Number: 21380073
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Bioproduction chemistry/Bioorganic chemistry
• Research Institution: Nagahama Institute of Bio-Science and Technology
• Principal Investigator: SAI Koushoku 長浜バイオ大学, バイオサイエンス研究科, 教授 (00263442)
• Co-Investigator(Renkei-kenkyūsha): IMAMURA Aya 長浜バイオ大学, バイオサイエンス学部, 講師 (50410965) ; IWANO Megumi 奈良先端科学技術大学院大学, バイオサイエンス研究科, 助教 (50160130)
• Project Period (FY): 2009 – 2011
• Keywords: 植物免疫 / エリシター / MAMP / PAMP / 活性酸素 / フラジェリン / タンパク質リン酸化 / 分子間相互作用

Research Abstract

We studied the induction mechanism of plant immune responses in rice. Several receptor kinases were identified as flagellin receptor. Complementary analysis in receptor gene-disruption mutants showed that FliRK2 functions as receptor for flagellin and the flagellin recognition signal is transduced through the protein phosphorylation into the cell. We also showed that the specific recognition of flagellin by rice is regulated by the glycans at^<178> Ser and^<183> Ser in flagellin prevent epitope recognition in rice.

Research Products

• [Journal Article] Genetic organization of the hrp gene cluster in Acidovorax avenae and novel effector proteins that elicit immune responses of rice (2012)
  • Author(s): Kondo, M., Yoshida, Y., Fujiwara, S., Nakajima, Y., Hirai, H., Takayama, S., Isogai, A., and Che, F. S.
  • Journal Title: Biosci. Biotech. Biochem Volume: 76 Pages: 129-138
  • Peer Reviewed
• [Journal Article] Glycosylation Regulates Specific Induction of Rice ImmuneResponses by Acidovorax avenae flagellin (2011)
  • Author(s): Hirai, H., Takai, R., Iwano, M., Nakai, M., Kondo, M., Takayama, S., Isogai, A., and Che, F. S.
  • Journal Title: J. Biol. Chem Volume: 289 Pages: 25519-25530
  • Peer Reviewed
• [Journal Article] Approaches to separate and identify polyADP-ribosylated proteins using poly(ADP-ribose) glycohydrolase-knockout Drosophila (2011)
  • Author(s): Nodono, H., Hamada, A., Kuroda, Y., Kamemura, K., Hasegawa, M., Komiya, T., Kondo, M., Che, F. S., Hanai, S., Miwa, M.
  • Journal Title: Methods Mol. Biol Volume: 780 Pages: 377-390
  • Peer Reviewed
• [Journal Article] Characterization of receptor proteins using affinity cross-linking with biotinylated ligands (2010)
  • Author(s): Shinya, T., Osada, T., Desaki, Y., Hatamoto, M., Yamanaka, Y., Hirano, H., Takai, R., Che, F. S., Kaku, H., Shibuya, N.
  • Journal Title: Plant Cell Physiol Volume: 51 Pages: 262-270
  • Peer Reviewed
• [Journal Article] The transcription factor OsNAC4 is a key positive regulator of plant hypersensitive cell death (2009)
  • Author(s): Kaneda, T., Taga, Y., Takai, R., Iwano, M., Matsui, H., Takayama, S., Isogai, A., Che F. S.
  • Journal Title: EMBO J Volume: 28 Pages: 926-936
  • Peer Reviewed
• [Journal Article] Phosphocholine-containing glycosyl inositol-phosphoceramides from Trichoderma viride induce defense responses in cultured rice cells (2009)
  • Author(s): Uchiyama, R., Aoki, K., Sugimoto, H., Taka, N., Katayama, T., Itonori, S., Sugita, M., Che, F. S., Kumagai, H., Yamamoto, K.
  • Journal Title: Biosci. Biotechnol. Biochem Volume: 73 Pages: 74-78
  • Peer Reviewed
• [Journal Article] Role of OsHSP90 and IREN, Ca2+dependent nuclease, in plant hypersensitive cell death induced by transcription factor OsNAC4 (2009)
  • Author(s): Taga, Y., Takai, R., Kaneda, T., Matsui, H., Isogai, A., Che, F. S.
  • Journal Title: Plant Signaling & Behavior Volume: 4 : 8 Pages: 1-3
  • Peer Reviewed
• [Journal Article] Coordination of plastid protein import and nuclear gene expression by plastid-to-nucleus retrograde signaling (2009)
  • Author(s): Kakizaki, T., Matsumura, H., Nakayama, K., Che F. S., Terauchi, R., Inaba, T.
  • Journal Title: Plant Physiol Volume: 151 Pages: 1339-1353
  • Peer Reviewed
• [Presentation] フラジェリン糖鎖構造によって制御されるイネによるAcidovorax avenae非病原性菌株のフラジェリン特異的認識とイネ免疫反応誘導機構 (2012)
  • Author(s): 平井洋行、中川幸彦、古川岳人、迹見勇樹、蔡晃植
  • Organizer: 日本農芸化学会2012年度大会
  • Place of Presentation: 京都
  • Year and Date: 2012-03-23
• [Presentation] Acidovorax avenaeK1菌株のフラジェリン糖鎖の非還元末端糖によるイネの免疫反応誘導特異性の制御 (2012)
  • Author(s): 中川幸彦、平井洋行、迹見勇樹、古川岳人、小野裕嗣、吉田充、蔡晃植
  • Organizer: 日本農芸化学会2012年度大会
  • Place of Presentation: 京都
  • Year and Date: 2012-03-23
• [Presentation] イネの免疫反応を誘導する病原細菌由来タンパク質の同定とその認識機構 (2012)
  • Author(s): 古川岳人、平井洋行、近藤真千子、蔡晃植
  • Organizer: 日本農芸化学会2012年度大会
  • Place of Presentation: 京都
  • Year and Date: 2012-03-23
• [Presentation] Acidovorax avenaeの病原性を異にするK1株およびN1141株のフラジェリン糖鎖構造 (2012)
  • Author(s): 吉田充、小野裕嗣、知久和寛、平井洋行、亀山眞由美、山本雅信、蔡晃植、石井忠、一瀬勇規
  • Organizer: 日本農芸化学会2012年度大会
  • Place of Presentation: 京都
  • Year and Date: 2012-03-23
• [Presentation] 植物病原細菌由来の鞭毛タンパク質フラジェリンのイネにおける認識機構の解析 (2012)
  • Author(s): 桂木雄也、小栗章成、柁山航介、梶本博文、田中佑佳、高井亮太、蔡晃植
  • Organizer: 日本植物生理学会2012年度大会
  • Place of Presentation: 京都
  • Year and Date: 2012-03-19
• [Presentation] Ca2+依存性プロテインキナーゼによる免疫反応である活性酸素発生の制御機構 (2012)
  • Author(s): 神村麻友、上坂有矢、韓宇龍、蔡晃植
  • Organizer: 日本植物生理学会2012年度大会
  • Place of Presentation: 京都
  • Year and Date: 2012-03-19
• [Presentation] Acidovorax avenaeのフラジェリンの糖鎖によって制御される植物免疫反応誘導特異性の解析 (2011)
  • Author(s): 平井洋行、古川岳人、蔡晃植
  • Organizer: 第34回日本分子生物学会
  • Place of Presentation: 横浜
  • Year and Date: 2011-12-16
• [Presentation] 植物病原細菌Acidovorax avenaeのHrp遺伝子群にコードされるイネ免疫反応誘導に関与する分泌タンパク質の機能解析 (2011)
  • Author(s): 近藤真千子、吉田裕貴、宮田千加、青井良介、佐々木悠、蔡晃植
  • Organizer: 第34回日本分子生物学会
  • Place of Presentation: 横浜
  • Year and Date: 2011-12-16
• [Book] 植物による鞭毛蛋白質フラジェリンの認識と転写制御を介した免疫反応の調節植物のシグナル伝達 (2010)
  • Author(s): 蔡晃植、柿本辰男、高山誠司、福田裕穂、松岡信
  • Total Pages: 133-138