2011 Fiscal Year Final Research Report
Identification of the diabetogenic gene and its related genes of high fat diet-induced diabetes by using novel mouse model
| Project/Area Number |
21380079
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Food science
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| Research Institution | Nagoya University |
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Principal Investigator |
HORIO Fumihiko 名古屋大学, 生命農学研究科, 教授 (20165591)
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| Co-Investigator(Renkei-kenkyūsha) |
OHNO Tamio 名古屋大学, 大学院・医学系研究科, 准教授 (90293620)
IKEGAMI Hiroshi 近畿大学, 医学部, 教授 (20221062)
MURAI Atsushi 名古屋大学, 大学院・生命農学研究科, 准教授 (10313975)
KOBAYASHI Misato 名古屋大学, 大学院・生命農学研究科, 助教 (20456586)
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| Project Period (FY) |
2009 – 2011
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| Keywords | 栄養生化学 |
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| Research Abstract |
SMXA-5 mouse is a novel animal model for type 2 diabetes induced by high fat diet-feeding. We have mapped the diabetic QTL (quantitative trait locus:T2dm2sa) on chromosome 2 of SMXA-5 mouse. The aim of this study is to choose the candidate gene for this diabetogenic gene and identify it as a causative gene. Simultaneously, the controlled genes by the isolated gene, which contribute to the development of diabetes,were screened.The chromosomal region which this diabetogenic gene exists was narrowed to 5Mb region of chromosome 2 by the phenotipic analyses of several diabetic congenic strains. As a candidate gene, Rapgef 4 was chosen. In the exon of Rapgef4 of SMXA-5 mouse, we observed a mutation causing the amino acid substitution. Further study is needed to clarify that this mutation of rapgef4 caused the development of diabetes.
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[Journal Article] Establishment of consomic strains derived from A/J and SM/J mice for genetic analysis of complex traits.2012
Author(s)
Ohno, T., Hata, K., Baba, T., Io, F., Kobayashi, M., Horio, F. and Nishimura, M.
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Journal Title
Mamm.Genome
Volume: 23
Pages: 764-769
Peer Reviewed
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[Journal Article] Coffee and caffeine improve insulin sensitivity and glucose tolerance in C57BL/6J mice fed a high-fat diet.2011
Author(s)
Matsuda, Y., Kobayashi, M., Yamauchi, R.,, Ojika,. M., Hiramitsu, M., Inoue, T.,..Katagiri, T., Murai A,. and Horio, F.
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Journal Title
Biosci.Biotechnol.Biochem
Volume: 75(12)
Pages: 2309-2315
Peer Reviewed
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[Journal Article] Coffee and caffeine ameliorate hyperglycemia, fatty liver, and inflammatory adipocytokine expression in spontaneously diabetic KK-Ay mice.2010
Author(s)
Yamauchi, R., Kobayashi, M., Matsuda, Y., Ojika, M., Shigeoka, S., Yamamoto, Y., Tou, Y., Inoue, T., Katagiri, T., Murai, A. and Horio, F
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Journal Title
J.Agric.Food Chem
Volume: 58
Pages: 5597-5603
Peer Reviewed
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[Journal Article] Genetic analysis of abdominal fat distribution in SM/J and A/J mice.2010
Author(s)
Kobayashi, M., Ohno, T., Hada, N., Fujiyoshi,M., Kuga, M., Nishimura, M., Murai, A. andHorio, F.
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Journal Title
J.Lipid Res.
Volume: 51
Pages: 3463-3469
Peer Reviewed
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[Journal Article] Confirmation of diabetes-related quantitative trait loci derived from SM/J and A/J mice by using congenic strains fed a high-carbohydrate or high-fat diet.2009
Author(s)
Kobayashi, M., Hada, N., Hoshino, H., Ozawa, T., Umeshita, K., Nishimura, M., Murai, A., Ohno, T. and Horio, F
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Journal Title
J.Nutr.Sci.Vitaminol.
Volume: 55
Pages: 257-263
Peer Reviewed
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[Journal Article] Body and major organ weights of A/J-Chr 11(SM) consomic mice.2009
Author(s)
Tanaka, S., Mizorogi, T., Nishijima, K., Kuwahara, S., Tsujio, M., Aoyama, H., Taguchi, C., Kobayashi, M., Horio, F. and Ohno, T
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Journal Title
Exp.Anim.
Volume: 58
Pages: 357-36
Peer Reviewed
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