Mechanism of regulation of cell differentiation in directly induced secondary xylem in trees

2011 Fiscal Year Final Research Report

• Project/Area Number: 21380107
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Wood science
• Research Institution: Tokyo University of Agriculture and Technology
• Principal Investigator: FUNADA Ryo 東京農工大学, 大学院・農学研究院, 教授 (20192734)
• Co-Investigator(Kenkyū-buntansha): KAJITA Shinya 東京農工大学, 大学院・農学研究院, 准教授 (40323753) ; WATANABE Ugai 千葉工業大学, 工学部, 准教授 (70337707)
• Project Period (FY): 2009 – 2011
• Keywords: 樹木二次木部 / 管状要素分化 / 木質バイオマス / 細胞壁 / 細胞骨格 / 微小管 / アクチンフィラメント / 培養細胞

Research Abstract

We induced directly secondary xylem like cells that formed developed bordered pits and spiral thickenings from cultured cells of woody plants such as hybrid poplar. In addition, we induced tracheary elements from suspended culture cells derived from needles of Cryptomeria japonica in vitro. Moreover, we analyzed the orientation and localization of microtubules and actin filaments using stable expression of genes for microtubule-associated proteins or second actin binding domain with green fluorescent protein. The microtubules play an important role in process of cell differentiation such as the determination of sites of pit formation.

Research Products

• [Journal Article] Cold stability of microtubules in wood forming tissues of conifers during seasons of active and dormant cambium (2012)
  • Author(s): Begum, S., Shibagaki, M., Furusawa, O., Nakaba, S., Yamagishi, Y., Yoshimoto, J., JinH. O., Sano, Y., Funada, R
  • Journal Title: Planta Volume: 235 Pages: 165-179
  • DOI: DOI:10.1007/s00425-011-1500-2
  • Peer Reviewed
• [Journal Article] Temporally and spatially controlled death of parenchyma cells in involved in heartwood formation in pith regions of branches of Robinia pseudoacacia var. inermis (2012)
  • Author(s): Nakaba, S., Yamagishi Y., Sano, Y., Funada, R
  • Journal Title: Journal of Wood Science Volume: 58 Pages: 69-76
  • DOI: DOI:10.1007/s10086-011-1221-y
  • Peer Reviewed
• [Journal Article] Differences in the timing of cell death, differentiation and function among three different types of ray parenchyma cells in the hardwood (2012)
  • Author(s): Nakaba, S., Begum, S., YamagishiY., Jin, HO., Kubo, T., Funada, R
  • Journal Title: Populus sieboldii xP. grandidentata, Trees Volume: 26 Pages: 743-750
  • DOI: DOI:10.1007/s00468-011-0640-0
  • Peer Reviewed
• [Journal Article] Plant regeneration by somatic embryogenesis from mature seeds of Magnolia obovata (2012)
  • Author(s): Park, I. S., Koiso, M., Morimoto, S., Kubo, T., Jin, H. O., Funada, R
  • Journal Title: Journal of Wood Science Volume: 58 Pages: 64-68
  • DOI: DOI:10.1007/s10086-011-1212-z
  • Peer Reviewed
• [Journal Article] Nuclear DNA fragmentation during cell death of short-lived ray tracheids in the conifer Pinus densiflora (2011)
  • Author(s): Nakaba, S., Kubo, T., Funada, R
  • Journal Title: Journal of Plant Research Volume: 124 Pages: 379-384
  • DOI: DOI:10.1007/s10265-010-0384-8
  • Peer Reviewed
• [Journal Article] Polyamines promote the development of embryonal-suspensor masses and the formation of somatic embryos in Picea glehnii (2011)
  • Author(s): Nakagawa, R., Kurushima, M., Matsui, M., Nakamura, R., Kubo, T., Funada, R
  • Journal Title: In Vitro Cellular Development Biology-Plant Volume: 47 Pages: 480-487
  • Peer Reviewed
• [Journal Article] 樹木の二次木部細胞におけるセルロースミクロフィブリルの配向制御 (2009)
  • Author(s): 船田良, 半智史
  • Journal Title: Cellulose Communications Volume: 16 Pages: 95-101
  • URL: http://iss.ndl.go.jp/books/R100000002-I000000101656-00
  • Peer Reviewed
• [Presentation] GFP-MAP4遺伝子の導入によるポプラ培養細胞からの管状要素分化過程における表層微小管の観察 (2012)
  • Author(s): 山岸祐介、内山大夢、吉本靖東、半智史、渡辺宇外、船田良
  • Organizer: 第62回日本木材学会大会
  • Place of Presentation: 札幌
  • Year and Date: 2012-03-16
• [Presentation] スギの培養細胞由来の管状要素誘導に関する研究 (2012)
  • Author(s): 内山大夢、山岸祐介、半智史、船田良
  • Organizer: 第62回日本木材学会大会
  • Place of Presentation: 札幌
  • Year and Date: 2012-03-15
• [Presentation] ホオノキ培養細胞における培地条件の細胞増殖およびmagnololの生成に与える影響 (2012)
  • Author(s): 大谷拓己、朴仁善、辻幸子、梶田真也、船田良
  • Organizer: 第62回日本木材学会大会
  • Place of Presentation: 札幌
  • Year and Date: 2012-03-15
• [Presentation] コブシの組織培養による植物体再生に関する研究 (2012)
  • Author(s): 岡加奈子、山岸祐介、小磯桃子、森本智美、朴仁善、藤田遼二、船田良
  • Organizer: 第62回日本木材学会大会
  • Place of Presentation: 札幌
  • Year and Date: 2012-03-15
• [Presentation] イチョウ、トガサワラおよびヌマスギの培養細胞を用いた管状要素誘導に関する研究 (2011)
  • Author(s): 内山大夢、山岸祐介、半智史、船田良
  • Organizer: 第61回日本木材学会大会
  • Place of Presentation: 京都
  • Year and Date: 2011-03-19
• [Presentation] GFP-ABD2遺伝子発現による交雑ポプラ培養細胞におけるアクチンの可視化 (2011)
  • Author(s): 山岸祐介、内山大夢、吉本靖東、半智史、渡辺宇外、船田良
  • Organizer: 第61回日本木材学会大会
  • Place of Presentation: 京都
  • Year and Date: 2011-03-19
• [Presentation] モクレン属の植物器官や植物器官由来のカルスにおけるmagnololとhonokiolの定量 (2011)
  • Author(s): 大谷拓己、朴仁善、山岸祐介、小磯桃子、辻幸子、梶田真也、船田良
  • Organizer: 第61回日本木材学会大会
  • Place of Presentation: 京都
  • Year and Date: 2011-03-19
• [Presentation] ホオノキの未成熟種子PEMs誘導における種子の最適な発達段階に関する研究 (2011)
  • Author(s): 小磯桃子、朴仁善、森本智美、山岸祐介、船田良
  • Organizer: 第61回日本木材学会大会
  • Place of Presentation: 京都
  • Year and Date: 2011-03-19
• [Presentation] Cell biological analysis of cell death in short-lived ray tracheids in the conifer (2010)
  • Author(s): Nakaba, S., Yoshimoto, J., Sano, Y., Kubo, T., Funada, R
  • Organizer: The 2010 International Workshop on Wood Biorefinery and Tree Biotechnology
  • Place of Presentation: Ornskoldsvik, Sweden
  • Year and Date: 2010-06-22
• [Presentation] ポプラ培養細胞からの二次木部の特徴をもつ管状要素の分化誘導 (2010)
  • Author(s): 山岸祐介、吉本靖東、内山大夢、吉田菜摘、半智史、渡辺宇外、久保隆文、船田良
  • Organizer: 第60回日本木材学会大会
  • Place of Presentation: 宮崎
  • Year and Date: 2010-03-18
• [Presentation] カキおよびホオノキにおけるin vitro管状要素誘導に関する研究 (2010)
  • Author(s): 吉田菜摘、山岸祐介、内山大夢、朴仁善、半智史、船田良
  • Organizer: 第60回日本木材学会大会
  • Place of Presentation: 宮崎
  • Year and Date: 2010-03-17
• [Book] 木質の形成(第2版) (2012)
  • Author(s): 船田良
  • Total Pages: 1-593
  • Publisher: 海青社
• [Remarks]
  • URL: http://www.tuat.ac.jp/.keisei/