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2011 Fiscal Year Final Research Report

Studies on the origin of exotic loaches and their impact on indigenous species

Research Project

  • PDF
Project/Area Number 21380114
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field General fisheries
Research InstitutionHokkaido University

Principal Investigator

ARAI Katsutoshi  北海道大学, 大学院・水産科学研究院, 教授 (00137902)

Co-Investigator(Kenkyū-buntansha) MURAKAMI Masaru  麻布大学, 獣医学部, 教授 (80271360)
YABE Mamoru  北海道大学, 大学院・水産科学研究院, 教授 (80174572)
Project Period (FY) 2009 – 2011
Keywords倍数体 / クローン / ミトコンドリアDNA / 反復配列 / マイクロサテライト / 交雑 / FISH / 生物多様性保全
Research Abstract

Loaches collected in Japan and adjacent area were genetically characterized by chromosome observation, genome size estimation and sequence analyses of mitochondrial and nuclear DNA markers. As a result, Japanese market samples were genetically identical or very similar to those distributed in China. As artificial inter-specific hybrids between indigenous loach and exotic mud loach species exhibit fertility in females, genetic contamination is worried in Japanese wild populations.

  • Research Products

    (21 results)

All 2012 2011 2010

All Journal Article (7 results) (of which Peer Reviewed: 7 results) Presentation (14 results)

  • [Journal Article] Induction of viable gynogenetic progeny using eggs and UV irradiated sperm from the Chinese tetraploid loach, Misgurnus anguillicaudatus2012

    • Author(s)
      Ya-Juan Li, Zhuo Yu, Ming-Zhao Zhang, Cong Qian, Syuiti Abe, Katsutoshi Arai
    • Journal Title

      Aquaculture International

      Volume: (印刷中)

    • Peer Reviewed
  • [Journal Article] Diploid clone produces unreduced diploid gametes but tetraploid clone generates reduced diploid gametes in the Misgurnus loach2012

    • Author(s)
      Kagayaki Morishima, Hiroyuki Yoshikawa, Katsutoshi Arai
    • Journal Title

      Biology of Reproduction

      Volume: 86巻33 Pages: 1-7

    • DOI

      DOI:10.1095/biolreprod.111.093302

    • Peer Reviewed
  • [Journal Article] Cold-shock eliminates female nucleus in fertilized eggs to induce androgenesis in the loach (Misgurnus anguillicaudatus), a teleost fish2011

    • Author(s)
      Kagayaki Morishima, Takafumi Fujimoto, Mami Sato, Ayako Kawae, Yan Zhao, Etsuro Yamaha, Katsutoshi Arai
    • Journal Title

      BMC Biotechnology

      Volume: 11巻 Pages: 116

    • DOI

      DOI:10.1186/1472-6750-11-116

    • Peer Reviewed
  • [Journal Article] Production of loach (Misgurnus anguillicaudatus) germ-line chimera using transplantation of primordial germ cells isolated from cryopreserved blastomeres2011

    • Author(s)
      George S Yasui, Takafumi Fujimoto, Suzu Sakao, Etsuro Yamaha, Katsutoshi Arai
    • Journal Title

      Journal of Animal Science

      Volume: 89巻 Pages: 2080-2388

    • DOI

      DOI:10.2527/jas.2011-4329

    • Peer Reviewed
  • [Journal Article] The origin of natural tetraploid loach Misgurnus anguillicaudatus (Teleostei : Cobitidae) inferred from meiotic chromosome configurations2011

    • Author(s)
      Ya-Juan Li, Zhuo Yu, Ming-Zhao Zhang, Cong Qian, Syuiti Abe, Katsutoshi Arai
    • Journal Title

      Genetica

      Volume: 139巻 Pages: 805-800

    • DOI

      DOI:10.1007/s10709-011-9585-x

    • Peer Reviewed
  • [Journal Article] Chromosome banding and FISH with rDNA probe in the diploid and tetraploid loach Misgurnus anguillicaudatus2010

    • Author(s)
      Ya-Juan Li, Yi Tian, Ming-Zhao Zhang, Ping-Ping Tian, Zhuo Yu, Syuiti Abe, Katsutoshi Arai
    • Journal Title

      Ichthyological Research

      Volume: 57巻 Pages: 358-366

    • DOI

      DOI:10.1007/s10228-010-0168-0

    • Peer Reviewed
  • [Journal Article] Restoration of the loach Misgurnus anguillicaudatus from cryopreserved diploid sperm and induced androgenesis2010

    • Author(s)
      George S Yasui, Takafumi Fujimoto, Katsutoshi Arai
    • Journal Title

      Aquaculture

      Volume: 308巻 Pages: S140-S144

    • DOI

      DOI:10.1016/j.aquaculture.2010.05.041

    • Peer Reviewed
  • [Presentation] 市販外来ドジョウの倍数性変異とDNAマーカーから見た起源2012

    • Author(s)
      趙岩・戸田昌邦・麻生真実・山田綾・荒井克俊(発表者)
    • Organizer
      平成24年度日本水産学会春季大会
    • Place of Presentation
      東京海洋大学(東京都)
    • Year and Date
      2012-03-27
  • [Presentation] 核DNAから見たクローンドジョウの識別法2011

    • Author(s)
      山田綾(発表者)・神門幸大・村上賢・荒井克俊
    • Organizer
      平成23年度日本水産学会北海道支部大会
    • Place of Presentation
      北海道大学(函館市)
    • Year and Date
      2011-11-16
  • [Presentation] RAG1遺伝子から見た自然クローンドジョウの雑種起源2011

    • Author(s)
      山田綾(発表者)・神門幸大・村上賢・荒井克俊
    • Organizer
      平成23年度日本水産学会秋季大会
    • Place of Presentation
      長崎大学(長崎市)
    • Year and Date
      2011-09-30
  • [Presentation] Motility, morphology, mitochondria and ATP content of diploid spermatozoa from sex reversed clonal diploid and neo-tetraploid loach, Misgurnus anguillicaudatus2011

    • Author(s)
      Yan Zhao (発表者), Martin Psenicka, Takafumi Fujimoto, Taiju Saito, George S Yasui, Etsuro Yamaha, Katsutoshi Arai
    • Organizer
      3^<rd> International Workshop on the Biology of Fish Gametes
    • Place of Presentation
      ブダペスト(ハンガリー)
    • Year and Date
      2011-09-08
  • [Presentation] Fertility and ploidy of gametes of diploid, triploid and tetraploid loaches, Misgurnus anguillicaudatus, in China2011

    • Author(s)
      Ya-Juan Li, Ming-Zhao Zhang, Cong Qian, Min Gao, Katsutoshi Arai (発表者)
    • Organizer
      3^<rd> International Workshop on the Biology of Fish Gametes
    • Place of Presentation
      ブダペスト(ハンガリー)
    • Year and Date
      2011-09-07
  • [Presentation] Induction of viable gynogenetic progeny using eggs and UV irradiated sperm of Chinese tetraploid loach, Misgurnus anguillicaudatus2011

    • Author(s)
      Ya-Juan Li, Zhuo Yu, Ming-Zhao Zhang, Cong Qian, Syuiti Abe, Katsutoshi Arai (発表者)
    • Organizer
      International Workshop, Diversification in Inland Finfish Aquaculture
    • Place of Presentation
      ピーセク(チェコ)
    • Year and Date
      2011-05-17
  • [Presentation] ドジョウ雌×カラドジョウ雄F1雑種の妊性と配偶子の倍数性2011

    • Author(s)
      光山成美・横山幸奈・藤本貴史・荒井克俊(発表者)
    • Organizer
      平成23年度日本水産学会春季大会
    • Place of Presentation
      東京海洋大学(東京都)
    • Year and Date
      2011-03-29
  • [Presentation] ドジョウ特異的なDNAマーカーの探索Part 22011

    • Author(s)
      神門幸大・堀口裕生・山田綾・藤本貴史・荒井克俊・村上賢
    • Organizer
      平成23年度日本水産学会春季大会
    • Place of Presentation
      東京海洋大学(東京都)
    • Year and Date
      2011-03-29
  • [Presentation] Genetic and cytogenetic characteristics of natural tetraploid loach Misgurnus anguillicaudatus in China2010

    • Author(s)
      Katsutoshi Arai (発表者)
    • Organizer
      The International Meeting on the Genetics of Polyploids
    • Place of Presentation
      University of Lisbon, Lisbon, Portgal
    • Year and Date
      2010-11-11
  • [Presentation] 中国産二倍体、三倍体および四倍体ドジョウ由来子孫の発生能力と染色体構成2010

    • Author(s)
      李雅娟・張明昭・周賀・荒井克俊(発表者)
    • Organizer
      平成22年度日本水産学会秋季大会
    • Place of Presentation
      京都大学(京都市)
    • Year and Date
      2010-09-24
  • [Presentation] ジョウ特異的なDNAマーカーの探索2010

    • Author(s)
      神門幸大・堀口裕生・山田綾・藤本貴史・荒井克俊・村上賢
    • Organizer
      平成22年度日本水産学会秋季大会
    • Place of Presentation
      京都大学(京都市)
    • Year and Date
      2010-09-23
  • [Presentation] 人為雌性発生子孫の発生能力と核型から推定した中国産自然四倍体ドジョウのゲノム構成2010

    • Author(s)
      李雅娟・于卓・銭聰・荒井克俊(発表者)
    • Organizer
      平成22年度日本水産学会秋季大会
    • Place of Presentation
      京都大学(京都市)
    • Year and Date
      2010-09-22
  • [Presentation] 中国産自然四倍体ドジョウ染色体のFISHと分染2010

    • Author(s)
      李雅娟・張明昭・魏傑・阿部周一・荒井克俊(発表者)
    • Organizer
      平成22年度日本水産学会春季大会
    • Place of Presentation
      日本大学(藤沢市)
    • Year and Date
      2010-03-29
  • [Presentation] 中国産四倍体ドジョウの減数分裂における染色体の挙動2010

    • Author(s)
      李雅娟・于卓・荒井克俊(発表者)
    • Organizer
      平成22年度日本水産学会春季大会
    • Place of Presentation
      日本大学(藤沢市)
    • Year and Date
      2010-03-27

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Published: 2013-07-31  

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