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2012 Fiscal Year Final Research Report

Studies on the mechanism of collagen fibril assembly in fish scales

Research Project

  • PDF
Project/Area Number 21380116
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field General fisheries
Research InstitutionHokkaido University

Principal Investigator

TAKAGI Yasuaki  北海道大学, 大学院・水産科学研究院, 教授 (10212002)

Co-Investigator(Kenkyū-buntansha) IKOMA Toshiyuki  東京工業大学, 大学院・理工学研究科, 准教授 (20370306)
Co-Investigator(Renkei-kenkyūsha) URA Kazuhiro  北海道大学, 大学院・水産科学研究院, 助教 (90360940)
Project Period (FY) 2009 – 2012
Keywords生理 / 生体材料 / 鱗 / 再生 / コラーゲン / 角膜
Research Abstract

Using MS analysis, scale SLRPs, which were possible regulators of 3-dimensinal architecture of teleost fish collagen, were identified. After the cDNA cloning of SLRPs, localization of their mRNAs were clarified. We also revealed a negative correlation between collagen denaturation temperature (DT) and contents of α3-subunit, as well as positive correlation between DT and hydroxylation ratio of proline residues in the collagen. Thus, these two factors seem to be the major regulators of DT of fish collagen. Artificial fibrillogenesis of collagen under magnetic field enabled a synthesis of a material having relatively aligned collagen fibrils, whose viscoelasticity and tensile strength were highest under 4 T and 12 T, respectively. High-density meso-porous new material was also synthesized using fish collagen.

  • Research Products

    (19 results)

All 2012 2011 2010 2009

All Journal Article (7 results) (of which Peer Reviewed: 3 results) Presentation (12 results)

  • [Journal Article] Developmental structure of the vertebral column, fins, scutes and scales in the bester, the hybrid of Beluga Huso huso and Sterlet Acipenser ruthenus2012

    • Author(s)
      Zhang X, Shimoda K, Ura K, Adachi S, Takagi Y
    • Journal Title

      Journal of Fish Biology

      Volume: 81 Pages: 1985-2004

    • DOI

      DOI:10.1111/j.1095-8649.2012.03451.x

    • Peer Reviewed
  • [Journal Article] Expression patterns of runx2, sparc and bgp during scale regeneration in the goldfish Carassius auratus, Journal of Experimental Biology2012

    • Author(s)
      Iimura K, Tohse H, Ura K, Takagi Y
    • Journal Title

      Part B, Molecular and Developmental Evolution

      Volume: 318 Pages: 190-198

    • DOI

      DOI:10.1002/jez.b.22005.

    • Peer Reviewed
  • [Journal Article] ウロコから眼:さかなのウロコのコラーゲンを利用して人工 角膜を造る2011

    • Author(s)
      都木靖彰
    • Journal Title

      化学と生物

      Volume: 49 Pages: 156-158

  • [Journal Article] 魚類コラーゲンと哺乳類コラーゲン-分子から見た共通点と相違点,そして魚類コラーゲン利用の水産業へのインパクト2011

    • Author(s)
      都木靖彰
    • Journal Title

      バイオインダストリー

      Volume: 28(11) Pages: 10-15

  • [Journal Article] Scale calcification in the goldfish in vitro: histological and quantitative analysis2010

    • Author(s)
      Ogawa N, Ura K, Takagi Y
    • Journal Title

      Fisheries Science

      Volume: 76 Pages: 189-198

    • DOI

      DOI 10.1007/s12562-009-0197-7

    • Peer Reviewed
  • [Journal Article] はじめに-なぜ,今,さかなコラーゲンか-2010

    • Author(s)
      都木靖彰
    • Journal Title

      マテリアルインテグレーション

      Volume: 23(02) Pages: 1-5

  • [Journal Article] 魚のうろこのコラーゲンの特徴-分子生物学-2010

    • Author(s)
      都木靖彰
    • Journal Title

      マテリアルインテグレーション

      Volume: 23(02) Pages: 12-16

  • [Presentation] ゼブラフィッシュ成魚におけるデルマトポンチン(Dpt)の発現解析2012

    • Author(s)
      檀永〓・飯村九林・佐藤哲郎・浦和寛・都木靖彰
    • Organizer
      (社)日本動物学会第83回大会
    • Place of Presentation
      大阪大学
    • Year and Date
      20120913-15
  • [Presentation] ゼブラフィッシュ Danio rerio の硬組織におけるデルマトポンチン(Dpt)の発現解析と機能解析2012

    • Author(s)
      檀永〓・浦和寛・都木靖彰
    • Organizer
      第7回バイオミネラリゼーションワークショップ
    • Place of Presentation
      東京大学理学部
    • Year and Date
      2012-12-01
  • [Presentation] ゼブラフィッシュ Danio rerio におけるデルマトポンチン (DPT) の発現解析2011

    • Author(s)
      檀永〓・飯村九林・浦和寛・都木靖彰
    • Organizer
      日本動物学会第82回大会
    • Place of Presentation
      旭川市大雪クリスタルホール
    • Year and Date
      20110921-23
  • [Presentation] LC-MS/MS analysis is a powerful tool to identify noncollagenous proteins in the scale basal plate of the goldfish Carassius auratus, 2nd International Workshop2011

    • Author(s)
      Takagi Y, Sekimoto H, Sugiyama A, Komatsu N, Ogawa N, Iimura K, Ura K
    • Organizer
      Interdisciplinary Approaches in Fish Skeletal Biology
    • Place of Presentation
      Tavira, Algarve, Portugal.
    • Year and Date
      20110426-28
  • [Presentation] Expression patterns of bone-related factors during scale regeneration in zebrafish Danio rerio, 2nd International Workshop2011

    • Author(s)
      Iimura K, Takagi Y
    • Organizer
      Interdisciplinary Approaches in Fish Skeletal Biology
    • Place of Presentation
      Tavira, Algarve, Portugal.
    • Year and Date
      20110426-28
  • [Presentation] 鱗の線維層板を構成するタンパク質の探索-1 非コラーゲン性タンパク質の網羅的同定の試み2010

    • Author(s)
      関本拓・小松典彦・杉山ありさ・小川展弘・浦和寛・都木靖彰
    • Organizer
      平成22年度日本水産学会秋季大会
    • Place of Presentation
      京都大学
    • Year and Date
      20100900
  • [Presentation] 鱗の線維層板を構成するタンパク質の探索-2 Small Leucine-Rich Proteoglycans (SLRPs) の cDNA クローニングおよび発現解析2010

    • Author(s)
      佐藤哲郎・浅井理沙・杉山ありさ・飯村九林・浦和寛・都木靖彰
    • Organizer
      平成22年度日本水産学会秋季大会
    • Place of Presentation
      京都大学
    • Year and Date
      20100900
  • [Presentation] Molecular mechanisms of cellular differentiation in teleost fish scales2010

    • Author(s)
      Iimura K, Tohse H, Ura K, Takagi Y
    • Organizer
      2nd International Sclerochronology Conference
    • Place of Presentation
      Johannes Gutenberg-Universitat, Mainz, Germany
    • Year and Date
      20100724-28
  • [Presentation] Proteomic and mRNA expression analyses in the teleost fish otolith and scale suggest organic control of rhythmic growth and biomineralization2010

    • Author(s)
      Takagi Y, Tohse H, Ogawa, Nagasawa H
    • Organizer
      2nd International Sclerochronology Conference
    • Place of Presentation
      Johannes Gutenberg-Universitat, Mainz, Germany
    • Year and Date
      20100724-28
  • [Presentation] キンギョ I 型コラーゲンに関する研究 1 I 型 コラーゲンの cDNA クローニングとその 発現解析2009

    • Author(s)
      飯村九林・真砂勇作・徳永啓太・長田光司・渡部晃・浦和寛・都木靖彰
    • Organizer
      平成21年度日本水産学会秋季大会
    • Place of Presentation
      いわて県民情報交流センター
    • Year and Date
      20090930-1003
  • [Presentation] キンギョI型コラーゲンに関する研究2生化学的解析と熱安定性2009

    • Author(s)
      小川展弘・生駒俊之・都木靖彰
    • Organizer
      平成21年度日本水産学会秋季大会
    • Place of Presentation
      いわて県民情報交流センター
    • Year and Date
      20090930-1003
  • [Presentation] (2009) キンギョ鱗の線維層板を構成する非コラーゲン性タンパク質の性状解析2009

    • Author(s)
      小松典彦・小川展弘・浦和寛・都木靖彰
    • Organizer
      (社)日本動物学会北海道支部第55回大会
    • Place of Presentation
      北海道大学水産学部
    • Year and Date
      2009-08-08

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Published: 2014-08-29  

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