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2011 Fiscal Year Final Research Report

Identification of Signaling Transmitter to Activate Nitrate Reductase in Vegetable leaves by Near-Infrared Hyperspectral Imaging System

Research Project

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Project/Area Number 21380156
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Agricultural environmental engineering
Research InstitutionKobe University

Principal Investigator

ITOH Hiromichi  神戸大学, 大学院・農学研究科, 准教授 (00258063)

Co-Investigator(Kenkyū-buntansha) SHIRAISHI Naomasa  神戸大学, 大学院・農学研究科, 助教 (00304121)
UNO Yuichi  神戸大学, 大学院・農学研究科, 准教授 (90304120)
Project Period (FY) 2009 – 2011
Keywords施設園芸 / 植物工場
Research Abstract

The purpose of this research is to clarify the relationship between nitrate concentration and variation in nitrate reductase(NR) gene expression. The tested plants were komatsuna. Absorption spectra at each pixel on leaf surface were measured by using a hpyerspectral imaging system. In order to derive mathematical models to estimate the nitrate concentration from absorption spectra, mean spectrum over pixels that were included in small leaf bit was measured. Nitrate concentration of the leaf bit was measured by HPLC. This measurement was applied to 459 leaf bits that included leaf blades and midribs. The mathematical models were developed for leaf blade or midrib separately. Correlation coefficient between the measured and estimated concentration of evaluation data showed more than 0. 91 in both the models. These models were applied to visualize the distribution image of nitrate concentration in a leaf. Then sections of high or low concentration were detected and were cut off from the leaf. Variations of NR gene expression in the leaf sections were measured by PT-PCR and agarose gel electrophoresis methods. Results of this analysis showed that there was not clear correlation between the nitrate concentration and variation of NR gene expression. However, variations of NR gene expression tended to increase with increment of nitrate concentration from initial concentration. It was clarified that the increment of nitrate concentration was considered as one of the factors to induce NR gene expression.

  • Research Products

    (29 results)

All 2012 2011 2010 2009

All Journal Article (11 results) (of which Peer Reviewed: 6 results) Presentation (17 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] 葉面内硝酸濃度分布計測法を援用した硝酸還元酵素遺伝子発現を誘導するシグナル伝達物質同定.葉面内硝酸イオン高濃度部と低濃度部の特定-2011

    • Author(s)
      伊藤博通, 松浦丈喜, 藤田裕子, 北秋広徳, 白石斉聖, 宇野雄一
    • Journal Title

      農業機械学会関西支部報

      Volume: 110 Pages: 44

  • [Journal Article] 葉面内硝酸濃度分布計測法を援用した硝酸還元酵素遺伝子発現を誘導するシグナル伝達物質同定.硝酸還元酵素遺伝子発現量と硝酸イオン濃度との関係-2011

    • Author(s)
      伊藤博通, 松浦丈喜, 北秋広徳, 藤田裕子, 白石斉聖, 宇野雄一
    • Journal Title

      農業機械学会関西支部報

      Volume: 110 Pages: 45

  • [Journal Article] Development of Method for Non-destructive Measurement of Nitrate Concentration in Vegetable Leaves by Near-infrared Spectroscopy2011

    • Author(s)
      Itoh, H., Tomita, H., Uno, Y., Shiraishi, N.
    • Journal Title

      Reprints of the 18th IFAC World Congress

      Volume: (CD-ROM) Pages: 6

    • Peer Reviewed
  • [Journal Article] Identification of Signaling Transmitter to Activate Nitrate Reductase in Vegetable Leaves by Near-Infrared Hyperspectral Imaging System2010

    • Author(s)
      Matsuura, H., Itoh, H., Tomoda, S., Shiraishi, N., Uno, Y.
    • Journal Title

      AGRICONTROL 2010 IFAC International Conference Preprints

      Volume: (CD-ROM) Pages: 6

    • Peer Reviewed
  • [Journal Article] 葉面内硝酸濃度分布計測法を援用した硝酸還元酵素活性を誘導するシグナル伝達物質同定.ハイパースペクトルイメージングシステムの開発-2010

    • Author(s)
      伊藤博通, 神田晋佑, 松浦丈喜, 白石斉聖, 宇野雄一, 友田小百合
    • Journal Title

      農業機械学会関西支部報

      Volume: 108 Pages: 62

  • [Journal Article] Development of Hyperspectral Imaging System to Measure Spatial Distribution of Nitrate Concentration in Vegetables2010

    • Author(s)
      Kanda, S., Itoh, H., Matsuura, H., Tomoda, S., Shiraishi, N., Sakai, K., Sasao, A.
    • Journal Title

      Proceedings of ISMAB 2010 JAPAN

      Volume: (CD-ROM) Pages: 6

    • Peer Reviewed
  • [Journal Article] Measurement of Nitrate Concentration Distribution in Vegetables by Near-Infrared Hyperspectral Imaging2010

    • Author(s)
      Itoh, H., Kanda, S., Matsuura, H., Shiraishi, N., Sakai, K. SASAO, A.
    • Journal Title

      Environment Control in Biology

      Volume: 48(2) Pages: 31-43

    • Peer Reviewed
  • [Journal Article] ハイパースペクトルカメラによる野菜株内硝酸イオン濃度分布計測2009

    • Author(s)
      伊藤博通, 神田晋佑, 松浦丈喜, 笹尾彰, 酒井憲司
    • Journal Title

      農業機械学会関西支部報

      Volume: 106 Pages: 32

  • [Journal Article] 光環境制御による葉菜中硝酸イオン濃度低減化技術の開発.近赤外線分光法による硝酸濃度非破壊計測法の開発2009

    • Author(s)
      伊藤博通, ツェンコヴァルミアナ, 白石斉聖, 宇野雄一, 松本拓也, 白居祐希
    • Journal Title

      農業機械学会関西支部報

      Volume: 106 Pages: 25

  • [Journal Article] Measurement of nitrate concentration distribution invegetables by hyperspectral camera2009

    • Author(s)
      Matsuura, H., Itoh, H., Kanda, S., Shiraishi, N., Sakai, K., Sasao, A.
    • Journal Title

      Proceedings of BIO-ROBOTICS IIII

      Volume: (CD-ROM) Pages: 6

    • Peer Reviewed
  • [Journal Article] Non-destructive measurement of nitrate concentration in vegetables by near infrared spectroscopy2009

    • Author(s)
      Matsumoto, T, Itoh, H., Shirai, Y., Shiraishi, N., Uno, Y.
    • Journal Title

      Proceedings of BIO-ROBOTICS IV

      Volume: (CD-ROM) Pages: 6

    • Peer Reviewed
  • [Presentation] 葉面内硝酸濃度分布計測法を援用した硝酸還元酵素遺伝子発現を誘導するシグナル伝達物質同定2012

    • Author(s)
      伊藤博通, 白石斉聖, 宇野雄一, 藤田裕子, 宮地大樹
    • Organizer
      農業機械学会関西支部第127回例会
    • Place of Presentation
      京都
    • Year and Date
      2012-03-05
  • [Presentation] 近赤外線分光法による植物体内硝酸イオン濃度非破壊計測法開発2011

    • Author(s)
      伊藤博通, 白石斉聖, 宇野雄一, 友田小百合, 濱田佳代荻野伸祐, 綾田晃士, 北秋広徳, 野村耕太, 藤田裕子
    • Organizer
      第12回計測自動制御学会システムインテグレーション部門
    • Place of Presentation
      京都
    • Year and Date
      2011-12-25
  • [Presentation] 葉面内硝酸濃度分布計測法を援用したNR遺伝子発現を誘導するシグナル伝達物質同定2011

    • Author(s)
      北秋広徳, 伊藤博通, 白石斉聖, 宇野雄一, 松浦丈喜, 藤田裕子
    • Organizer
      日本生物環境工学会2010年北海道大会
    • Place of Presentation
      札幌
    • Year and Date
      2011-09-08
  • [Presentation] Development of Method for Non-destructive Measurement of Nitrate Concentration in Vegetable Leaves by Near-infrared Spectroscopy2011

    • Author(s)
      Itoh, H. Tomita, H. Uno, Y., Shiraishi, N.
    • Organizer
      18th World Congress of the International Federation of Automatic Control
    • Place of Presentation
      Milano
    • Year and Date
      2011-08-29
  • [Presentation] 葉面内硝酸濃度分布計測法を援用した硝酸還元酵素遺伝子発現を誘導するシグナル伝達物質同定.葉面内硝酸イオン高濃度部と低濃度部の特定-2011

    • Author(s)
      伊藤博通, 松浦丈喜, 藤田裕子, 北秋広徳, 白石斉聖, 宇野雄一
    • Organizer
      農業機械学会関西支部第125回例会
    • Place of Presentation
      堺
    • Year and Date
      2011-03-02
  • [Presentation] 葉面内硝酸濃度分布計測法を援用した硝酸還元酵素遺伝子発現を誘導するシグナル伝達物質同定.硝酸還元酵素遺伝子発現量と硝酸イオン濃度との関係-2011

    • Author(s)
      伊藤博通, 松浦丈喜, 北秋広徳, 藤田裕子, 白石斉聖, 宇野雄一
    • Organizer
      農業機械学会関西支部第125回例会
    • Place of Presentation
      堺
    • Year and Date
      2011-03-02
  • [Presentation] Identification of Signaling Transmitter to Activate NitrateReductase in Vegetable Leaves by Near-Infrared Hyperspectral Imaging System2010

    • Author(s)
      Matsuura, H., Itoh, H., Tomoda, S., Shiraishi, N., Uno, Y.
    • Organizer
      AGRICONTROL 2010 IFAC International Conference
    • Place of Presentation
      Kyoto
    • Year and Date
      2010-12-07
  • [Presentation] 葉面内硝酸濃度分布計測法を援用した硝酸還元酵素活性誘導のシグナル伝達物質同定.葉面内硝酸イオン濃度分布の経時変化計測.2010

    • Author(s)
      松浦丈喜, 伊藤博通, 友田小百合, 白石斉聖, 宇野雄一
    • Organizer
      第69回農業機械学会年次大会
    • Place of Presentation
      松山
    • Year and Date
      2010-09-14
  • [Presentation] 硝酸還元酵素活性の誘導シグナル伝達物質同定のための葉面内硝酸濃度分布計測2010

    • Author(s)
      松浦丈喜, 伊藤博通, 友田小百合, 白石斉聖, 宇野雄一
    • Organizer
      日本生物環境工学会2010年京都大会
    • Place of Presentation
      京都
    • Year and Date
      2010-09-08
  • [Presentation] ハイパースペクトルカメラを使用した野菜葉内硝酸イオン濃度分布計測2010

    • Author(s)
      松浦丈喜, 伊藤博通, 神田晋佑, 笹尾彰, 酒井憲司
    • Organizer
      農業機械学会関西支部第124回例会
    • Place of Presentation
      岡山
    • Year and Date
      2010-08-31
  • [Presentation] Measurement of nitrate concentration distribution in vegetable leaves by near-infrared hyperspectral imaging2010

    • Author(s)
      Matsuura, H., Tomoda, S., Shiraishi, N., Uno, Y. Itoh, H.
    • Organizer
      1st International Symposium on the Nitrogen Nutrition of Plants
    • Place of Presentation
      Inuyam
    • Year and Date
      2010-07-27
  • [Presentation] Development of Hyperspectral Imaging System to Measure Spatial Distribution of Nitrate Concentration in Vegetables2010

    • Author(s)
      Kanda, S., Itoh, H., Matsuura, H., Tomoda, S., Shiraishi, N., Sakai, K., Sasao, A
    • Organizer
      International Symposium on Machinery and Mechatronics for Agriculture and Biosystems Engineering
    • Place of Presentation
      JAPAN, Fukuoka
    • Year and Date
      2010-04-06
  • [Presentation] ハイパースペクトルカメラによる野菜葉面内硝酸イオン濃度分布の計測法開発2009

    • Author(s)
      伊藤博通, 神田晋佑, 松浦丈喜, 笹尾彰, 酒井憲司
    • Organizer
      農業環境工学関連学会2009年合同大会
    • Place of Presentation
      東京
    • Year and Date
      2009-09-16
  • [Presentation] Measurement of nitrate concentration distribution in vegetables by hyperspectral camera2009

    • Author(s)
      Matsuura, H., Itoh, H., Kanda, S., Shiraishi, N., Sakai K., Sasao, A.
    • Organizer
      Bio-Robotics IV
    • Place of Presentation
      USA
    • Year and Date
      2009-09-10
  • [Presentation] Non-destructive measurement of nitrate concentration in vegetables by near infrared spectroscopy2009

    • Author(s)
      Matsumoto, T., Itoh H., Shirai Y., Shiraishi, N., Uno, Y.
    • Organizer
      Bio-Robotics IV
    • Place of Presentation
      Illinois, USA
    • Year and Date
      2009-09-10
  • [Presentation] ハイパースペクトルカメラによる野菜内硝酸イオン濃度分布の計測法開発2009

    • Author(s)
      松浦丈喜, 伊藤博通, 神田晋佑, 白石斉聖, 酒井憲司, 笹尾彰
    • Organizer
      日本生物環境工学会2009年福岡大会
    • Place of Presentation
      福岡
    • Year and Date
      2009-09-08
  • [Presentation] 近赤外線分光法による野菜内硝酸イオン濃度非破壊計測法の開発2009

    • Author(s)
      松本拓也, 伊藤博通, 白居祐希, 白石斉聖, 宇野雄一
    • Organizer
      日本生物環境工学会2009年福岡大会
    • Place of Presentation
      福岡
    • Year and Date
      2009-09-08
  • [Patent(Industrial Property Rights)] 透過型近赤外線分光計測装置および透過型近赤外線分光計測方法2010

    • Inventor(s)
      伊藤博通,白石斉聖、宇野雄一
    • Industrial Property Rights Holder
      神戸大学
    • Industrial Property Number
      特願、2010-198383
    • Filing Date
      2010-09-03

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Published: 2013-07-31  

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