2012 Fiscal Year Final Research Report
Analysis of mitochondrial function in mammalian oocytes using electrochemical microscopy
| Project/Area Number |
21380171
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied animal science
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| Research Institution | Yamagata University |
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Principal Investigator |
ABE Hiroyuki 山形大学, 大学院・理工学研究科, 教授 (10375199)
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| Co-Investigator(Kenkyū-buntansha) |
YOKOO Masaki 秋田県立大学, 生物資源科学部, 准教授 (10396541)
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| Co-Investigator(Renkei-kenkyūsha) |
SHIKU Hitoshi 東北大学, 大学院・環境科学研究科, 准教授 (10361164)
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| Project Period (FY) |
2009 – 2012
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| Keywords | 電気化学計測 / 細胞呼吸 / ミトコンドリア / 卵成熟 / 胚発生 |
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| Research Abstract |
Oxygen consumption is a ubiquitous parameter which can provide valuable informationon metabolic mechanisms, and oocyte and embryo quality. The aim of this study was 1) todevelop a device to measure cellular respiration of single oocytes and embryos based onscanning electrochemical microscopy (SECM) and 2) to assess the mitochondrial respiration function, such as oxygen consumption and gene expression of cytochrome coxidase (Cox) in single oocytes and embryos at different developmental stages. We succeeded in development of a modified SECM measuring system including Pt-microdiskelectrodes and respiration assay medium can monitor the oxygen consumption rate ofsingle oocytes and embryos. Using this system, we can measure respiration activity bysingle embryos and oocytes of bovine and mice at different developmental stages. Development of mitochondrial respiration activity, such as membrane potential of mitochondria and gene expression of Cox, corresponds to the increase of oxygen consumption rates during the development of embryos.
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Research Products
(61 results)
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[Journal Article] Secretoglobin 3A2 suppresses bleomycin-induced pulmonary fibrosis by TGFbeta signaling down-regulation2011
Author(s)
Kurotani R., Okumura S., Matsubara T., Yokoyama U., BuckleyJ.R., Tomita T., Kezuka K., NaganoT., Esposito D., Taylor T.E., GilletteW.K., Ishikawa Y., Abe H., Ward J.M., Kimura S.
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Journal Title
J. Biol. Chem
Volume: 286(22)
Pages: 19682-19692
Peer Reviewed
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[Journal Article] Viability of porcine embryos after vitrification using water-soluble pullulan fims2010
Author(s)
Sakagami N., Yamamoto T., AkiyamaK., Nakazawa Y., Kojima N., NishidaK., Yokomizo S., Takagi Y., Abe H.,Suzuki C., Yoshioka K.
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Journal Title
J. Reprod. Dev.
Volume: 56(2)
Pages: 279-284
Peer Reviewed
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[Journal Article] A microfluidic dual capillaryprobe to collect messenger RNA fromadherent cells and spheroids2009
Author(s)
Shiku H., Yamakawa T., Nashimoto Y., Takahashi Y., Torisawa Y., Yasukawa T., Ito-Sasaki T., Yokoo M., Abe H., Kambara H., Matsue T.
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Journal Title
Anal. Biochem.
Volume: 385
Pages: 138-142
Peer Reviewed
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