Regeneration ofthe chick organs and the application for breeding by use of a stem cell

2012 Fiscal Year Final Research Report

• Project/Area Number: 21380173
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied animal science
• Research Institution: Shinshu University
• Principal Investigator: KAGAMI Hiroshi 信州大学, 農学部, 教授 (80308303)
• Co-Investigator(Kenkyū-buntansha): ONO Tamao 信州大学, 農学部, 教授 (10177264)
• Project Period (FY): 2009 – 2012
• Keywords: 育種 / 胚性幹細胞 / 体性幹細胞 / 臓器 / 器官 / 再生 / 家禽

Research Abstract

Stem cells which retained pluripotency was isolated from the chick embryos or somatic cells. The obtained stem cells were used as donor cell for making germline and somatic chicmeras. The embryonic stem cells were derived from area pellucida of stage X blastoderm. The somatic stem cells were obtained from bone marrowcells or adipocytes. The germline chimeras were effectively generated by the embryonic stem cells. The bone marrow cells or adipocyte could be regenerated into vein, muscle or bone cells. Due to these results, the stem cells could be useful tools for future poultry breeding.

Research Products

• [Journal Article] Development, Differentiation and Manipulation of Chicken Germ Cells (2013)
  • Author(s): Nakamura, Y., Kagami, H., Tagami, T
  • Journal Title: Development, Growth & Differentiation Volume: 55 Pages: 20-40
  • Peer Reviewed
• [Journal Article] X-irradiation Removes Endogenous Primordial Germ Cells (PGCs) and Increases Germline Transmission of Donor PGCs in Chimeric Chickens (2012)
  • Author(s): Nakamura, Y., Usui, F., Miyahara, D., Mori, T., Ono, T., Kagami, H., Takeda, K., Nirasawa, K., Tagami, T
  • Journal Title: Journal of Reproduction and Development Volume: 58 Pages: 432-437
  • Peer Reviewed
• [Journal Article] A Simple Culture method of Chicken Blastodermal Cells for Germline Transmission (2011)
  • Author(s): Matsubara, Y., Hirota, A., Sobajima, H., Kagami, H., Tagami, T. and Yasue. H.
  • Journal Title: Journal of Poultry Science Volume: 48 Pages: 14-18
  • Peer Reviewed
• [Journal Article] Efficient System for Preservation and Regeneration of Genetic Resources in Chicken: Concurrent Store of Primordial Germ Cells and Live Animals from early Embryos of A Rare Indigenous Fowl (Gifujidoro). Reproduction (2010)
  • Author(s): Nakamura, Y., Usui, F., Miyahara, D., Mori, F., Ono, T., Takeda, K., Nirasawa, K., Kagami, H and Tagami, T.
  • Journal Title: Fertility and Development Volume: 22 Pages: 1237-1344
  • Peer Reviewed
• [Journal Article] Germline Replacement by Transfer of Primordial Germ Cells into Partially Sterilized Embryos in the Chicken. (2010)
  • Author(s): Nakamura, Y., Usui, F., Ono, T., Takeda, K., Nirasawa, K., Kagami, H and Tagami, T.
  • Journal Title: Biology of Reproduction Volume: 83 Pages: 130-137
  • Peer Reviewed
• [Journal Article] A Novel Concentration System of Chicken Stem Cells by Bone Marrow Side Population Cells. (2010)
  • Author(s): Usui, F., Nakamura, Y., Yamamoto, Y., Tatsumi, K., Tominari, K., Ono, T. and Kagami, H
  • Journal Title: Journal of Poultry Science Volume: 47 Pages: 53-56
  • Peer Reviewed
• [Presentation] 幹細胞の畜産への活用 (2013)
  • Author(s): 鏡味裕
  • Organizer: 日本畜産学会第116回大会
  • Place of Presentation: 広島
  • Year and Date: 2013-03-29
• [Presentation] Future Possibility and Current Status of AnimalStem Cell Technology (2012)
  • Author(s): Kagami, H.
  • Organizer: The 2ndJapan-Korea-China Joint Symposium
  • Place of Presentation: Society for Reproduction, Tsukuba
  • Year and Date: 2012-09-05
• [Presentation] Perspective for Animal Biotechnology. (2012)
  • Author(s): Kagami, H.
  • Organizer: International Symposium on Animal Biotechnology
  • Place of Presentation: Minamiminowa
  • Year and Date: 2012-01-31
• [Presentation] 幹細胞分化制御による鳥類生殖工学への応用 (2011)
  • Author(s): 鏡味裕、中村隼明、宮原大地、森貴史、渡辺晴陽、大西翔、砂岡耕平、田中健生、田村芽衣、小野珠乙、松原悠子、韮澤圭二郎、田上貴寛.
  • Organizer: 104回日本繁殖生物学会大会
  • Place of Presentation: 盛岡
  • Year and Date: 2011-09-02
• [Presentation] ニワトリ幹細胞の培養条件検討と単一幹細胞培養への活用. (2011)
  • Author(s): 鏡味裕、中村隼明、宮原大地、森貴史、渡辺晴陽、今井一樹、中村幸夫、小野珠乙、松原悠子、中村俊、小柴満美子、韮澤圭二郎、田上貴寛.
  • Organizer: 日本畜産学会114回大会
  • Place of Presentation: 十和田
  • Year and Date: 2011-08-26
• [Presentation] 迅速簡便法による各ニワトリ品種の始原生殖細胞の採取と特性評価 (2011)
  • Author(s): 鏡味裕、臼井文武、中村隼明、山本耕裕、宮原大地、森貴史、渡辺晴陽、今井一樹、小野珠乙、松原悠子、韮澤圭二郎、田上貴寛.
  • Organizer: 日本家禽学会 2011 年度春季大会
  • Place of Presentation: 厚木
  • Year and Date: 2011-03-30
• [Presentation] Effect ofX-ray Irradiation of Primordoal Germ Cell-depletion in Chicken Embryos. (2011)
  • Author(s): Nakamura, Y., Usui, F., Miyahara, D., Mori, T., Watanabe, H., Ono, T., Takeda, K., Nirasawa, Kagami, H., Tagami, T
  • Organizer: th Asia Pacific Poultry Conference
  • Place of Presentation: Taipei
  • Year and Date: 2011-03-22
• [Presentation] Mixed Sex Chimeras and Sex Reversal. (2011)
  • Author(s): Kagami, H.
  • Organizer: 1stInternational Symposium on Conservation & Propagation of Endangered Species of Birds.
  • Place of Presentation: Abu Dhabi
  • Year and Date: 2011-02-08
• [Presentation] Avian Stem Cells and the Application for Biotechnology. (2010)
  • Author(s): Kagami, H., Usui, F., Nakamura, Y., Kashiwagi, M., Yamamoto, Y., Ono, T., Tagami, T.,Nirasawa, K., Matsubara, Y., Petitte, J.N.
  • Organizer: 43rd Annual Meeting for the Japanese Society of Developmental Biologist
  • Place of Presentation: Kyoto
  • Year and Date: 2010-06-21
• [Presentation] ニワトリ始原生殖細胞の利用:移動様式の解明と凍結保存・移植・除去技術の確立. (2010)
  • Author(s): 中村隼明、宮原大地、森貴史、今井一樹、渡辺晴陽、小野珠乙、武田久美子、韮澤圭二郎、鏡味裕、田上貴寛.
  • Organizer: 日本分子生物学会第10回春季シンポジウム
  • Place of Presentation: 松島
  • Year and Date: 2010-06-07
• [Presentation] ニワトリ幹細胞・始原生殖細胞の遺伝的特徴解析と分化制御の試み (2010)
  • Author(s): 宮原大地、今井一樹、森貴史、坂田〓子、坪井友子、都築明香里、中村隼明、臼井文武、山本耕裕、田上貴寛、韮澤圭二郎、松原悠子、川口耕一郎、藤井博、小野珠乙、鏡味裕
  • Organizer: 日本分子生物学会第10回春季シンポジウム
  • Place of Presentation: 松島
  • Year and Date: 2010-06-07
• [Presentation] ニワトリ幹細胞・始原生殖細胞の培養と発生・分化における特徴把握 (2010)
  • Author(s): 鏡味裕、臼井文武、中村隼明、柏木まや、伊東陽平、山本耕裕、田上貴寛、韮澤圭二郎、松原悠子、小野珠乙
  • Organizer: 日本畜産学会 112回大会
  • Place of Presentation: 東京
  • Year and Date: 2010-03-29
• [Presentation] 始原生殖細胞の新規採取法の開発と生殖細胞キメラ作出への活用.発生・分化における特徴把握. (2010)
  • Author(s): 鏡味裕、柏木まや、大屋智子、臼井文武、中村隼明、山本耕裕、小野珠乙
  • Organizer: 日本畜産学会 112回大会
  • Place of Presentation: 始原生殖細胞の新規採取法の開発と生殖細胞キメラ作出への活用.発生・分化における特徴把握.
  • Year and Date: 2010-03-29
• [Presentation] 幹細胞・生殖細胞の遺伝的特徴把握と鳥類における発生・再生制御への活用の試み. (2009)
  • Author(s): 鏡味裕、臼井文武、中村隼明、山本耕裕、大友朝子、柏木まや、田上貴寛、韮澤圭二郎、松原悠子、小野珠乙
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-10
• [Presentation] 始原生殖細胞とその収集時に温存したニワトリ胚からの個体再生 (2009)
  • Author(s): 中村隼明、臼井文武、柏木まや、坂田詢子、小野珠乙、武田久美子、韮澤圭二郎、鏡味裕、田上貴寛
  • Organizer: 日本畜産学会 111回大会
  • Place of Presentation: 那覇
  • Year and Date: 2009-09-28
• [Presentation] 胚盤葉明域中央部への局所的 UV 照射及び生殖新月環への UV 照射を用いたレシピエント胚由来PGCs 数減少の試み (2009)
  • Author(s): 柏木まや、中村隼明、山本耕裕、臼井文武、川越雄太、松原和衛、小野珠乙、鏡味裕
  • Organizer: 日本畜産学会111回大会
  • Place of Presentation: 那覇
  • Year and Date: 2009-09-28
• [Presentation] ニワトリ幹細胞及び始原生殖細胞の発生に伴う遺伝子発現の解析と分化制御への活用の試み (2009)
  • Author(s): 鏡味裕、臼井文武、中村隼明、山本耕裕、大友朝子、柏木まや、片倉真沙美、田上貴寛、韮澤圭二郎、松原悠子、小野珠乙
  • Organizer: 日本畜産学会111回大会
  • Place of Presentation: 那覇
  • Year and Date: 2009-09-28
• [Presentation] 凍結融解操作がニワトリ始原生殖細胞の生存性および生殖巣移住能へ及ぼす影響 (2009)
  • Author(s): 中村隼明、臼井文武、柏木まや、坂田詢子、小野珠乙、武田久美子、韮澤圭二郎、鏡味裕、田上貴寛
  • Organizer: 日本家禽学会 2009 年度秋季大会
  • Place of Presentation: 那覇
  • Year and Date: 2009-09-26
• [Presentation] 造血幹細胞同定を目的とした致死量放射線量の探索. (2009)
  • Author(s): 臼井文武、中村隼明、小野珠乙、鏡味裕.
  • Organizer: 日本家禽学会 2009 年度秋季大会
  • Place of Presentation: 那覇
  • Year and Date: 2009-09-26
• [Presentation] 宿主の内因性始原生殖細胞の除去によるドナー由来の生殖系譜に置換されたキメラニワトリの作出用. (2009)
  • Author(s): 中村隼明、臼井文武、柏木まや、坂田詢子、小野珠乙、武田久美子、韮澤圭二郎、鏡味裕、田上貴寛
  • Organizer: 第102回日本繁殖生物学会大会
  • Place of Presentation: 奈良
  • Year and Date: 2009-09-10
• [Book] 鳥類の受精、発生、胚操作. 新動物生殖学(佐藤英明編著) (2011)
  • Author(s): 鏡味裕
  • Total Pages: 151-160
  • Publisher: 朝倉書店
• [Book] ニワトリ初期胚における DNA メチル化状態の変化. DNA 多型 Vol. 17(日本 DNA 多型学会編) (2009)
  • Author(s): 臼井文武、小野珠乙、鏡味裕
  • Total Pages: 216-218
  • Publisher: 東洋書店
• [Remarks]
  • URL: http://karamatsu.shinshu-u.ac.jp/lab/ono_kagami/hasseiken.htm