2012 Fiscal Year Final Research Report
Cryopreservation and improvement of developmental ability of porcine primordial oocytes
| Project/Area Number |
21380175
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Applied animal science
|
|---|
| Research Institution | National Institute of Agrobiological Sciences |
|---|
Principal Investigator |
KANEKO Hiroyuki 独立行政法人農業生物資源研究所, 発生分化研究ユニット, 上級研究員 (60343993)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
KIKUCHI Kazuhiro 独立行政法人・農業生物資源研究所, 動物科学研究領域・動物発生分化研究ユニット, 上級研究員 (20360456)
KASHIWAZAKI Naomi 麻布大学, 獣医学部, 教授 (90298232)
|
|---|
| Project Period (FY) |
2009 – 2012
|
| Keywords | 原始卵胞卵 / 原始卵胞卵 / 体外培養系 / 体外培養系 / ガラス化冷却 / 超低温保存 |
|---|
| Research Abstract |
Primordial follicles act as stores for ovarian follicles and are a potential resource of oocytes for medical, agricultural and zoological purposes. In the present study, we have demonstrated that fusion of cytoplasts prepared from competent porcine oocytes enhances the developmental ability of oocytes derived from porcine primordial follicles grafted into nude mice. We also have explored a vitrification protocol that allows porcine ovary tissue to maintain its ability to produce antral follicles after xenografting. Oocytes retrieved from porcine xenografts were proven to have the abilities of in-vitro maturation and fertilization. These methods are necessary for utilization of primordial oocytes in large mammals
|
Research Products
(11 results)
-
-
-
-
-
[Journal Article] Noguchi J, Ito J, Kashiwazaki N, Kikuchi K. Production of viable piglets for the first time using sperm derived from ectopic testicular xenografts2010
Author(s)
Nakai M, Kaneko H, Somfai T, Maedomari N, Ozawa M, Noguchi J, Ito J, Kashiwazaki N, Kikuchi K
-
Journal Title
Reprod
Volume: 139
Pages: 331-335
DOI
Peer Reviewed
-
-
-
-
-
-
