2011 Fiscal Year Final Research Report
Improvement of Photobiological Hydrogen Production of Cyanobacteria by Expressing Different Types of Nitrogenase through Genetic Engineering
| Project/Area Number |
21380200
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Boundary agriculture
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| Research Institution | Kanagawa University |
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Principal Investigator |
SAKURAI Hidehiro 神奈川大学, 光合成水素生産研究所, 客員教授研 (10063645)
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| Co-Investigator(Kenkyū-buntansha) |
INOUE Kazuhit 神奈川大学, 理学部, 教授 (20221088)
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| Project Period (FY) |
2009 – 2011
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| Keywords | シアノバクテリア / 水素 / 光合成 / ニトロゲナーゼ / 再生可能エネルギー / バナ |
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| Research Abstract |
In order to improve the nitrogenase-based photobiological hydrogen production by cyanobacteria, we aimed at creating cyanobacteria mutants that preferentially express V or Fe-only type nitrogenase in place of Mo type, by genetic engineering. We have knocked out the Mo type nitrogenase gene of the cyanobacteria that have either Mo type and V type, or solely Mo type. We have prepared plasmids that harbor V or Fe-only type genes. In order to decrease the Mo possibly eluted from culture glass bottles into the culture medium, we developed hydrogen-gas-barrier plastic bags.
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